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Literature summary extracted from

  • Anderson, S.L.; Schirf, V.; McAlister-Henn, L.
    Effect of AMP on mRNA binding by yeast NAD+-specific isocitrate dehydrogenase (2002), Biochemistry, 41, 7065-7073.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.41 AMP activation of the enzyme and reduction of enzyme inhibition by mitochondrial mRNA, highly enhanced by presence of isocitrate, complex formation, overview Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.41 mRNA enzyme specifically binds to 5'-untranslated regions of yeast mitochondrial mRNAs, and transcripts containing these regions allosterically inhibit the enzyme, which can be relieved by activator AMP in presence of isocitrate, complex formation, overview Saccharomyces cerevisiae
1.1.1.42 additional information activity is not affected by the nonspecific binding of the mitochondrial isozyme, not the cytosolic one, to 5'-untranslated regions of yeast mitochondrial mRNAs Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.41 mitochondrion
-
 Saccharomyces cerevisiae 5739
-
1.1.1.42 cytosol
-
 Saccharomyces cerevisiae 5829
-
1.1.1.42 mitochondrion
-
 Saccharomyces cerevisiae 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.41 Mg2+
-
 Saccharomyces cerevisiae
1.1.1.42 Mg2+
-
 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.41 isocitrate + NAD+ Saccharomyces cerevisiae enzyme has a regulatory role in the tricarboxylic cycle 2-oxoglutarate + CO2 + NADH + H+
-
 ?
1.1.1.41 additional information Saccharomyces cerevisiae allosteric regulation, enzyme specifically binds to 5'-untranslated regions of yeast mitochondrial mRNAs, and transcripts containing these regions allosterically inhibit the enzyme, which can be relieved by activator AMP in presence of isocitrate, complex formation, overview ?
-
 ?
1.1.1.42 isocitrate + NADP+ Saccharomyces cerevisiae
-
 2-oxoglutarate + CO2 + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.41 Saccharomyces cerevisiae
-
-
-
1.1.1.42 Saccharomyces cerevisiae
-
 cytosolic isozyme IDP1, and mitochondrial isozyme IDP2
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.41 isocitrate + NAD+
-
 Saccharomyces cerevisiae 2-oxoglutarate + CO2 + NADH + H+
-
 ?
1.1.1.41 isocitrate + NAD+ enzyme has a regulatory role in the tricarboxylic cycle Saccharomyces cerevisiae 2-oxoglutarate + CO2 + NADH + H+
-
 ?
1.1.1.41 additional information allosteric regulation, enzyme specifically binds to 5'-untranslated regions of yeast mitochondrial mRNAs, and transcripts containing these regions allosterically inhibit the enzyme, which can be relieved by activator AMP in presence of isocitrate, complex formation, overview Saccharomyces cerevisiae ?
-
 ?
1.1.1.42 D-isocitrate + NADP+
-
 Saccharomyces cerevisiae 2-oxoglutarate + CO2 + NADPH
-
 ?
1.1.1.42 isocitrate + NADP+
-
 Saccharomyces cerevisiae 2-oxoglutarate + CO2 + NADPH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.41 IDH
-
 Saccharomyces cerevisiae
1.1.1.41 NAD+-specific isocitrate dehydrogenase
-
 Saccharomyces cerevisiae
1.1.1.42 IDP
-
 Saccharomyces cerevisiae
1.1.1.42 NADP+-specific isocitrate dehydrogenase
-
 Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.41 7.4
-
 assay at Saccharomyces cerevisiae
1.1.1.42 7.8
-
 assay at Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.41 NAD+ dependent on Saccharomyces cerevisiae