EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.51 | D242G | azide activates the mutant, while it does not activate the wild-type enzyme, formate activates mutant higher than wild-type enzyme, the reaction catalysed by the mutant depends less on temperature | Saccharolobus solfataricus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.51 | Saccharolobus solfataricus | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.2.1.51 | an alpha-L-fucoside + H2O = L-fucose + an alcohol | mechanism | Saccharolobus solfataricus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.51 | p-nitrophenyl alpha-L-fucoside + H2O | - |
Saccharolobus solfataricus | p-nitrophenol + alpha-L-fucose | - |
? |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.51 | additional information | - |
comparison of temperature range depending of wild-type and mutant enzyme, the reaction catalyzed by the mutant D242G depends less on temperature | Saccharolobus solfataricus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.51 | 0.24 | - |
p-nitrophenyl alpha-L-fucoside | pH 6.5, 65°C, D242G mutant | Saccharolobus solfataricus | |
3.2.1.51 | 245 | - |
p-nitrophenyl alpha-L-fucoside | pH 6.5, 65°C, wild-type enzyme | Saccharolobus solfataricus |