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Literature summary extracted from

  • Groce, S.L.; Miller-Rodeberg, M.A.; Lipscomb, J.D.
    Single-turnover kinetics of homoprotocatechuate 2,3-dioxygenase (2004), Biochemistry, 43, 15141-15153.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.15 0.015
-
 4-nitrocatechol pH 7.5, 22°C Brevibacterium fuscum
1.13.11.15 0.025
-
 3,4-Dihydroxyphenylacetate pH 7.5, 22°C Brevibacterium fuscum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.15 Fe2+
-
 Brevibacterium fuscum

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.15 Brevibacterium fuscum
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.13.11.15 3,4-dihydroxyphenylacetate + O2 = 2-hydroxy-5-carboxymethylmuconate semialdehyde substrate binding occurs in a four-step process, transient intermediates and mechanism Brevibacterium fuscum
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.15 3,4-dihydroxyphenylacetate + O2
-
 Brevibacterium fuscum 2-hydroxy-5-carboxymethyl-cis,cis-muconate semialdehyde
-
 ?
1.13.11.15 4-nitrocatechol + O2
-
 Brevibacterium fuscum ? cleavage in proximal extradiol position ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.15 0.05
-
 4-nitrocatechol pH 7.5, 4°C Brevibacterium fuscum
1.13.11.15 0.4
-
 4-nitrocatechol pH 7.5, 22°C Brevibacterium fuscum
1.13.11.15 3.2
-
 3,4-Dihydroxyphenylacetate pH 7.5, 4°C Brevibacterium fuscum
1.13.11.15 10
-
 3,4-Dihydroxyphenylacetate pH 7.5, 22°C Brevibacterium fuscum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.15 6.9
-
 substrate 4-nitrocatechol Brevibacterium fuscum
1.13.11.15 8.4
-
 substrate 3,4-dihydroxyphenylacetate Brevibacterium fuscum