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Literature summary extracted from

  • Kim, E.J.; Feng, J.; Bramlett, M.R.; Lindahl, P.A.
    Evidence for a proton transfer network and a required persulfide-bond-forming cysteine residue in Ni-containing carbon monoxide dehydrogenases (2004), Biochemistry, 43, 5728-5734.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.2.7.4 A121H/H122A 11% of wild type activity Moorella thermoacetica
1.2.7.4 C316S 0.3% of wild type activity Moorella thermoacetica
1.2.7.4 E115H/H116A 24% of wild type activity Moorella thermoacetica
1.2.7.4 H113A 44% of wild type activity, in presence of imidazole, 45% of wild type activity Moorella thermoacetica
1.2.7.4 H113A/H116A/H119A no activity Moorella thermoacetica
1.2.7.4 H113A/H119A 15% of wild type activity Moorella thermoacetica
1.2.7.4 H116A 6% of wild type activity, in presence of imidazole, 3% of wild type activity Moorella thermoacetica
1.2.7.4 H116A/G117H 0.8% of wild type activity Moorella thermoacetica
1.2.7.4 H116C 46% of wild type activity Moorella thermoacetica
1.2.7.4 H116D 0.4% of wild type activity Moorella thermoacetica
1.2.7.4 H119A 27% of wild type activity Moorella thermoacetica
1.2.7.4 H122A 3% of wild type activity Moorella thermoacetica
1.2.7.4 H122A/A123H 72% of wild type activity Moorella thermoacetica
1.2.7.4 K587A 42% of wild type activity Moorella thermoacetica
1.2.7.4 K587A/H113A 0.7% of wild type activity Moorella thermoacetica
1.2.7.4 N284A 41% of wild type activity Moorella thermoacetica
1.2.7.4 N284A/H119A 36% of wild type activity Moorella thermoacetica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.7.4 Iron
-
 Moorella thermoacetica
1.2.7.4 Nickel
-
 Moorella thermoacetica

Organism

EC Number Organism UniProt Comment Textmining
1.2.7.4 Moorella thermoacetica
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.7.4 CO + H2O + acceptor
-
 Moorella thermoacetica CO2 + reduced acceptor
-
 r

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.7.4 9
-
-
 Moorella thermoacetica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.7.4 8
-
 about 60% of activity at pH 9.0 Moorella thermoacetica