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Literature summary extracted from

  • Igamberdiev, A.U.; Gardestrom, P.
    Regulation of NAD- and NADP-dependent isocitrate dehydrogenases by reduction levels of pyridine nucleotides in mitochondria and cytosol of pea leaves (2003), Biochim. Biophys. Acta, 1606, 117-125.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.41 additional information the enzyme is not affected by NADP+, HCO3-, and 2-oxoglutarate Pisum sativum
1.1.1.42 additional information the mitochondrial isozyme is light-inducible, while the cytosolic one is not Pisum sativum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.41 additional information the enzyme is not affected by NADP+, HCO3-, and 2-oxoglutarate Pisum sativum
1.1.1.41 NADH competitive Pisum sativum
1.1.1.41 NADPH noncompetitive Pisum sativum
1.1.1.42 2-oxoglutarate
-
 Pisum sativum
1.1.1.42 isocitrate reverse reaction Pisum sativum
1.1.1.42 additional information poor inhibition of the forward reaction by 2-oxoglutarate, no inhibition by CO2 Pisum sativum
1.1.1.42 NADH noncompetitive Pisum sativum
1.1.1.42 NADP+ reverse reaction Pisum sativum
1.1.1.42 NADPH forward reaction Pisum sativum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.41 0.12
-
 isocitrate pH 7.5 Pisum sativum
1.1.1.41 0.2
-
 NAD+ pH 7.5 Pisum sativum
1.1.1.42 0.003
-
 NADP+ mitochondrial isozyme, forward reaction, pH 8.0 Pisum sativum
1.1.1.42 0.004
-
 NADP+ cytosolic isozyme, forward reaction, pH 8.0 Pisum sativum
1.1.1.42 0.004
-
 isocitrate mitochondrial isozyme, forward reaction, pH 8.0 Pisum sativum
1.1.1.42 0.006
-
 isocitrate cytosolic isozyme, forward reaction, pH 8.0 Pisum sativum
1.1.1.42 0.009
-
 NADPH mitochondrial isozyme, reverse reaction, pH 7.0 Pisum sativum
1.1.1.42 0.017
-
 2-oxoglutarate mitochondrial isozyme, reverse reaction, pH 7.0 Pisum sativum
1.1.1.42 0.024
-
 NADPH cytosolic isozyme, reverse reaction, pH 7.0 Pisum sativum
1.1.1.42 0.026
-
 2-oxoglutarate cytosolic isozyme, reverse reaction, pH 7.0 Pisum sativum
1.1.1.42 0.2
-
 CO2 mitochondrial isozyme, reverse reaction, pH 7.0 Pisum sativum
1.1.1.42 1.6
-
 CO2 cytosolic isozyme, reverse reaction, pH 7.0 Pisum sativum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.41 mitochondrion
-
 Pisum sativum 5739
-
1.1.1.42 cytosol
-
 Pisum sativum 5829
-
1.1.1.42 mitochondrion light-inducible isozyme Pisum sativum 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.41 Mn2+
-
 Pisum sativum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.41 isocitrate + NAD+ Pisum sativum pyridine nucleotide contents in mitochondria and cytosol, regulation, overview 2-oxoglutarate + CO2 + NADH + H+
-
 ir
1.1.1.42 isocitrate + NADP+ Pisum sativum the cytosolic isozyme supplies 2-oxoglutarate for the photorespiratory ammonia fixation, pyridine nucleotide contents in mitochondria and cytosol, regulation, overview 2-oxoglutarate + CO2 + NADPH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.41 Pisum sativum
-
 cv. 294 040 Märgärt Kelvedon Wonder
-
1.1.1.42 Pisum sativum
-
 cv. 294 040 Märgärt Kelvedon Wonder, one cytosolic and one mitochondrial isozyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.41 partially by mitochondrion isolation in a Percoll gradient, and ammonium sulfate precipitation in the 60-80% fraction, DEAE cellulose chromatography, and desalting Pisum sativum
1.1.1.42 cytosolic and mitochondrial isozymes Pisum sativum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.41 leaf green and etiolated Pisum sativum
-
1.1.1.42 leaf green and etiolated Pisum sativum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.41 0.084
-
 mitochondria from green leaves, forward reaction Pisum sativum
1.1.1.41 0.096
-
 mitochondria from etiolated leaves, forward reaction Pisum sativum
1.1.1.42 0.038
-
 mitochondria from etiolated leaves, forward reaction at pH 7.5 Pisum sativum
1.1.1.42 0.072
-
 mitochondria from green leaves, forward reaction at pH 7.5 Pisum sativum

Storage Stability

EC Number Storage Stability Organism
1.1.1.41 -80°C, partially purified enzyme, several months, stable Pisum sativum
1.1.1.42 -80°C, partially purified cytosolic and mitochondrial isozymes, several months, stable Pisum sativum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.41 isocitrate + NAD+ pyridine nucleotide contents in mitochondria and cytosol, regulation, overview Pisum sativum 2-oxoglutarate + CO2 + NADH + H+
-
 ir
1.1.1.41 threo-Ds-isocitrate + NAD+
-
 Pisum sativum 2-oxoglutarate + CO2 + NADH
-
 ir
1.1.1.42 isocitrate + NADP+
-
 Pisum sativum 2-oxoglutarate + CO2 + NADPH + H+
-
 r
1.1.1.42 isocitrate + NADP+ the cytosolic isozyme supplies 2-oxoglutarate for the photorespiratory ammonia fixation, pyridine nucleotide contents in mitochondria and cytosol, regulation, overview Pisum sativum 2-oxoglutarate + CO2 + NADPH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.41 NAD+-dependent ICDH
-
 Pisum sativum
1.1.1.41 NAD-dependent isocitrate dehydrogenase
-
 Pisum sativum
1.1.1.41 NAD-ICDH
-
 Pisum sativum
1.1.1.42 NADP-dependent isocitrate dehydrogenase
-
 Pisum sativum
1.1.1.42 NADP-ICDH
-
 Pisum sativum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.41 7.5
-
-
 Pisum sativum
1.1.1.42 6.3 6.5 reverse reaction, cytosolic isozyme Pisum sativum
1.1.1.42 6.8 7 reverse reaction, mitochondrial isozyme Pisum sativum
1.1.1.42 8 9 forward reaction, cytosolic and mitochondrial isozymes Pisum sativum

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.41 NAD+ specific for Pisum sativum
1.1.1.42 additional information no activity with NAD+ and NADH Pisum sativum
1.1.1.42 NADP+ absolutely specific for, forward reaction Pisum sativum
1.1.1.42 NADPH absolutely specific for, reverse reaction Pisum sativum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.41 0.18
-
 NADH pH 7.5 Pisum sativum
1.1.1.41 0.26
-
 NADPH pH 7.5 Pisum sativum
1.1.1.42 0.005
-
 NADPH mitochondrial isozyme, forward reaction, pH 8.0 Pisum sativum
1.1.1.42 0.005
-
 isocitrate mitochondrial isozyme, reverse reaction, pH 7.0 Pisum sativum
1.1.1.42 0.006
-
 NADP+ mitochondrial isozyme, reverse reaction, pH 7.0 Pisum sativum
1.1.1.42 0.007
-
 NADPH cytosolic isozyme, forward reaction, pH 8.0 Pisum sativum
1.1.1.42 0.012
-
 isocitrate cytosolic isozyme, reverse reaction, pH 7.0 Pisum sativum
1.1.1.42 0.021
-
 NADP+ cytosolic isozyme, reverse reaction, pH 7.0 Pisum sativum
1.1.1.42 0.06
-
 NADH mitochondrial isozyme, forward reaction, pH 8.0 Pisum sativum
1.1.1.42 0.08
-
 NADH cytosolic isozyme, forward reaction, pH 8.0 Pisum sativum
1.1.1.42 1
-
 2-oxoglutarate above, cytosolic isozyme, forward reaction, pH 8.0 Pisum sativum
1.1.1.42 1
-
 2-oxoglutarate above, mitochondrial isozyme, forward reaction, pH 8.0 Pisum sativum