EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.1.5.2 | D354N | site-directed mutagenesis, 9% of wild-type activity, mutant enzyme can be reconstituted with PQQ and Ca2+, Sr2+, or Ba2+, but not with Mg2+, which functions as a competitive inhibitor, in contrary to the wild-type enzyme | Escherichia coli |
1.1.5.2 | D354N/N355D | site-directed mutagenesis, 10% of wild-type activity, mutant enzyme can be reconstituted with PQQ and Ca2+, Sr2+, or Ba2+, but not with Mg2+, which functions as a competitive inhibitor, in contrary to the wild-type enzyme | Escherichia coli |
1.1.5.2 | N355D | site-directed mutagenesis, 25% of wild-type activity, mutant enzyme can be reconstituted with PQQ and Ca2+, Sr2+, or Ba2+, but not with Mg2+, which functions as a competitive inhibitor, in contrary to the wild-type enzyme | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.5.2 | Ba2+ | competitive to Mg2+, wild-type enzyme | Escherichia coli | |
1.1.5.2 | Ca2+ | competitive to Mg2+, wild-type enzyme | Escherichia coli | |
1.1.5.2 | Mg2+ | competitive to Ca2+, Sr2+, or Ba2+, mutants D354N, N355D, and D354N/N355D | Escherichia coli | |
1.1.5.2 | Sr2+ | competitive to Mg2+, wild-type enzyme | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.5.2 | additional information | - |
additional information | metal binding kinetics, wild-type and mutant enzymes, Km for the different substrates of mutant enzymes with different metal ions bound, overview | Escherichia coli | |
1.1.5.2 | 2.8 | - |
D-glucose | recombinant wild-type enzyme with bound Mg2+, pH 6.5, 25°C | Escherichia coli | |
1.1.5.2 | 3.5 | - |
2-deoxy-D-glucose | recombinant wild-type enzyme with bound Mg2+, pH 6.5, 25°C | Escherichia coli | |
1.1.5.2 | 17 | - |
D-xylose | recombinant wild-type enzyme with bound Mg2+, pH 6.5, 25°C | Escherichia coli | |
1.1.5.2 | 17.5 | - |
D-galactose | recombinant wild-type enzyme with bound Mg2+, pH 6.5, 25°C | Escherichia coli | |
1.1.5.2 | 31 | - |
L-arabinose | recombinant wild-type enzyme with bound Mg2+, pH 6.5, 25°C | Escherichia coli | |
1.1.5.2 | 79 | - |
3-O-methyl-D-glucose | recombinant wild-type enzyme with bound Mg2+, pH 6.5, 25°C | Escherichia coli | |
1.1.5.2 | 116 | - |
D-mannose | recombinant wild-type enzyme with bound Mg2+, pH 6.5, 25°C | Escherichia coli | |
1.1.5.2 | 166 | - |
D-ribose | recombinant wild-type enzyme with bound Mg2+, pH 6.5, 25°C | Escherichia coli |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.1.5.2 | membrane | - |
Escherichia coli | 16020 | - |
1.1.5.2 | periplasm | - |
Escherichia coli | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.5.2 | Ba2+ | mutants D354N, N355D, and D354N/N355D | Escherichia coli | |
1.1.5.2 | Ca2+ | mutants D354N, N355D, and D354N/N355D | Escherichia coli | |
1.1.5.2 | Mg2+ | required, bound at the active site, cannot be substituted by Ca2+, Sr2+, or Ba2+ | Escherichia coli | |
1.1.5.2 | Sr2+ | mutants D354N, N355D, and D354N/N355D | Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.5.2 | D-glucose + ubiquinone | Escherichia coli | transfers electrons to the cytochrome oxidase through ubiquinone in the electron transport chain | D-glucono-1,5-lactone + ubiquinol | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.5.2 | Escherichia coli | P15877 | - |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.1.5.2 | D-glucose + ubiquinone = D-glucono-1,5-lactone + ubiquinol | active site structure | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.5.2 | 2-deoxy-D-glucose + ubiquinone | - |
Escherichia coli | 2-deoxy-D-glucono-1,5-lactone + ubiquinol | - |
? | |
1.1.5.2 | 3-O-methyl-D-glucose + ubiquinone | - |
Escherichia coli | 3-O-methyl-D-glucono-1,5-lactone + ubiquinol | - |
? | |
1.1.5.2 | D-galactose + ubiquinone | - |
Escherichia coli | D-galactono-1,5-lactone + ubiquinol | - |
? | |
1.1.5.2 | D-glucose + ubiquinone | - |
Escherichia coli | D-glucono-1,5-lactone + ubiquinol | - |
? | |
1.1.5.2 | D-glucose + ubiquinone | transfers electrons to the cytochrome oxidase through ubiquinone in the electron transport chain | Escherichia coli | D-glucono-1,5-lactone + ubiquinol | - |
? | |
1.1.5.2 | D-mannose + ubiquinone | - |
Escherichia coli | ? + ubiquinol | - |
? | |
1.1.5.2 | D-ribose + ubiquinone | - |
Escherichia coli | ? + ubiquinol | - |
? | |
1.1.5.2 | D-xylose + ubiquinone | - |
Escherichia coli | D-xylono-1,5-lactone + ubiquinol | - |
? | |
1.1.5.2 | L-arabinose + ubiquinone | - |
Escherichia coli | L-arabino-1,5-lactone + ubiquinol | - |
? | |
1.1.5.2 | additional information | substrate specificity of recombinant wild-type and mutant enzymes | Escherichia coli | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.5.2 | GDH | - |
Escherichia coli |
1.1.5.2 | membrane glucose dehydrogenase | - |
Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.5.2 | 25 | - |
assay at | Escherichia coli |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.5.2 | 40.5 | - |
10 min, 50% inactivation of recombinant mutant D354N apoenzyme | Escherichia coli |
1.1.5.2 | 40.8 | - |
10 min, 50% inactivation of recombinant wild-type apoenzyme | Escherichia coli |
1.1.5.2 | 41 | - |
10 min, 50% inactivation of recombinant mutant D354N/N355D apoenzyme | Escherichia coli |
1.1.5.2 | 43.2 | - |
10 min, 50% inactivation of recombinant mutant N355D apoenzyme | Escherichia coli |
1.1.5.2 | 48.6 | - |
10 min, 50% inactivation of recombinant mutant D354N/N355D holoenzyme | Escherichia coli |
1.1.5.2 | 49 | - |
10 min, 50% inactivation of recombinant mutant D354N holoenzyme | Escherichia coli |
1.1.5.2 | 51 | - |
10 min, 50% inactivation of recombinant wild-type holoenzyme | Escherichia coli |
1.1.5.2 | 55 | - |
10 min, 50% inactivation of recombinant mutant N355D holoenzyme | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.5.2 | 6.5 | - |
assay at | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.5.2 | pyrroloquinoline quinone | i.e. PQQ, dependent on, Escherichia coli needs to be reconstituted with PQQ for activity | Escherichia coli |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.5.2 | additional information | - |
additional information | inhibitory effects of the different metal ions on recombinant wild-type and mutant enzymes | Escherichia coli |