EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.5.1.81 | H250N | KM-value for N-acetyl-D-leucine is 6fold higher than the wild-type value, turnover number is 0.0246% of the wild-type value. Below 1% of the relative activity compared with wild-type enzyme.The zinc content of the mutant enzyme is 0.7 gatom per mol, compared to 2.3 gatom per mol for the wild-type enzyme | Achromobacter xylosoxidans |
3.5.1.81 | H251N | approximately 30% of the activity of wild-type enzyme. KM-value for N-acetyl-D-leucine is 32% of the wild-type value, turnover number is 48% of the wild-type value. | Achromobacter xylosoxidans |
3.5.1.81 | H67I | no detectable activity | Achromobacter xylosoxidans |
3.5.1.81 | H67N | KM-value for N-acetyl-D-leucine is 75% of the wild-type value, turnover number is 0.0012% of the wild-type value. The zinc content of the mutant enzyme is 2.2 gatom per mol, compared to 2.3 gatom per mol for the wild-type enzyme | Achromobacter xylosoxidans |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.81 | 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide | 16% inhibition at 1 mM, 45% inhibition at 10 mM | Achromobacter xylosoxidans | |
3.5.1.81 | 2,4,6-Trinitrobenzene-1-sulfonate | 40% inhihition at 1 mM, 86% inhibition at 10 mM | Achromobacter xylosoxidans | |
3.5.1.81 | 3-Bromopyruvate | 10 mM, 28% inhibition | Achromobacter xylosoxidans | |
3.5.1.81 | 4-(2-aminoethyl)benzenesulfonyl fluoride hydrochloride | 17% inhibition at 1 mM, 42% inhibition at 10 mM | Achromobacter xylosoxidans | |
3.5.1.81 | 5,5'-dithiobis(2-nitrobenzoic acid) | 10 mM, 41% inhibition | Achromobacter xylosoxidans | |
3.5.1.81 | diethyl dicarbonate | 93% inhibition by 93 mM, complete inhibition by 10 mM | Achromobacter xylosoxidans | |
3.5.1.81 | iodoacetate | 10 mM, 37% inhibition | Achromobacter xylosoxidans | |
3.5.1.81 | N-Acetylimidazole | 88% inhibition at 1 mM, 96% inhibition at 10 mM | Achromobacter xylosoxidans | |
3.5.1.81 | NEM | 10 mM, 27% inhibition | Achromobacter xylosoxidans | |
3.5.1.81 | Phenylglyoxal | 55% inhibition at 1 mM, 98% inhibition at 10 mM | Achromobacter xylosoxidans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.81 | 0.61 | - |
N-acetyl-D-leucine | mutant enzyme H251N | Achromobacter xylosoxidans | |
3.5.1.81 | 1.44 | - |
N-acetyl-D-leucine | mutant enzyme H67N | Achromobacter xylosoxidans | |
3.5.1.81 | 1.93 | - |
N-acetyl-D-leucine | wild-type enzyme | Achromobacter xylosoxidans | |
3.5.1.81 | 11.6 | - |
N-acetyl-D-leucine | mutant enzyme H250N | Achromobacter xylosoxidans |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.81 | Zinc | wild-type enzyme contains 2.3 gatom of zinc per mol of enzyme, mutant enzyme H67N contains 2.2 gatom of zinc per mol of enzyme, mutant enzyme H250N contains 0.7 gatom of zinc per mol of enzyme | Achromobacter xylosoxidans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.1.81 | 52000 | - |
x * 52000, wild-type enzyme and mutant enzymes H167N, H67I, H250N and H251N, SDS-PAGE | Achromobacter xylosoxidans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.81 | Achromobacter xylosoxidans | - |
subsp. xylosoxydans A-6 | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.81 | N-acetyl-D-leucine + H2O | - |
Achromobacter xylosoxidans | acetate + D-leucine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.1.81 | ? | x * 52000, wild-type enzyme and mutant enzymes H167N, H67I, H250N and H251N, SDS-PAGE | Achromobacter xylosoxidans |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.81 | 0.0147 | - |
N-acetyl-D-leucine | mutant enzyme H67N | Achromobacter xylosoxidans | |
3.5.1.81 | 0.297 | - |
N-acetyl-D-leucine | mutant enzyme H250N | Achromobacter xylosoxidans | |
3.5.1.81 | 593.3 | - |
N-acetyl-D-leucine | mutant enzyme H251N | Achromobacter xylosoxidans | |
3.5.1.81 | 1225 | - |
N-acetyl-D-leucine | wild-type enzyme | Achromobacter xylosoxidans |