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Literature summary extracted from

  • Nishimoto, M.; Kubota, M.; Tsuji, M.; Mori, H.; Kimura, A.; Matsui, H.; Chiba, S.
    Purification and substrate specificity of honeybee, Apis mellifera L., alpha-glucosidase III (2001), Biosci. Biotechnol. Biochem., 65, 1610-1616.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.20 additional information
-
 additional information determination of subsite affinities, kinetics Apis mellifera
3.2.1.20 2 3 phenyl alpha-D-glucoside pH 5.5, 35°C Apis mellifera
3.2.1.20 10
-
 maltotriose pH 5.5, 35°C Apis mellifera
3.2.1.20 11
-
 maltose pH 5.5, 35°C Apis mellifera
3.2.1.20 13
-
 4-nitrophenyl alpha-D-glucopyranoside pH 5.5, 35°C Apis mellifera
3.2.1.20 16
-
 maltotetraose pH 5.5, 35°C Apis mellifera
3.2.1.20 30
-
 sucrose pH 5.5, 35°C Apis mellifera
3.2.1.20 31
-
 turanose pH 5.5, 35°C Apis mellifera
3.2.1.20 44
-
 nigerose pH 5.5, 35°C Apis mellifera
3.2.1.20 56
-
 isomaltose pH 5.5, 35°C Apis mellifera
3.2.1.20 63
-
 maltopentaose pH 5.5, 35°C Apis mellifera
3.2.1.20 63
-
 maltohexaose pH 5.5, 35°C Apis mellifera
3.2.1.20 67
-
 kojibiose pH 5.5, 35°C Apis mellifera

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.20 68000
-
 about, gel filtration Apis mellifera

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Apis mellifera
-
 alpha-glucosidase III
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.20 glycoprotein about 7.4% carbohydrate content Apis mellifera

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20 to homogeneity, by salting-out chromatography and 5 additional chromatographic steps Apis mellifera

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.20 additional information
-
-
 Apis mellifera
3.2.1.20 11.3
-
 purified enzyme Apis mellifera

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 4-nitrophenyl alpha-D-glucopyranoside + H2O high activity Apis mellifera 4-nitrophenol + alpha-D-glucopyranose
-
 ?
3.2.1.20 isomaltose + H2O low activity Apis mellifera 2 alpha-D-glucose
-
 ?
3.2.1.20 kojibiose + H2O
-
 Apis mellifera alpha-D-glucose + D-glucose
-
 ?
3.2.1.20 maltohexaose + H2O low activity Apis mellifera maltopentaose + D-glucose
-
 ?
3.2.1.20 maltopentaose + H2O
-
 Apis mellifera alpha-D-glucose + ?
-
 ?
3.2.1.20 maltose + H2O
-
 Apis mellifera alpha-D-glucose + D-glucose
-
 ?
3.2.1.20 maltotetraose + H2O
-
 Apis mellifera alpha-D-glucose
-
 ?
3.2.1.20 maltotriose + H2O best substrate Apis mellifera maltose + alpha-D-glucose
-
 ?
3.2.1.20 additional information maltooligosachharides of four or more units of polymerization are slowly hydrolyzed Apis mellifera ?
-
 ?
3.2.1.20 nigerose + H2O
-
 Apis mellifera 2 alpha-D-glucose
-
 ?
3.2.1.20 phenyl alpha-D-glucoside + H2O
-
 Apis mellifera phenol + alpha-D-glucose
-
 ?
3.2.1.20 phenyl maltoside + H2O
-
 Apis mellifera phenol + alpha-D-glucose
-
 ?
3.2.1.20 sucrose + H2O
-
 Apis mellifera alpha-D-glucose + beta-D-fructose
-
 ?
3.2.1.20 turanose + H2O
-
 Apis mellifera alpha-D-glucose + D-fructose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.20 monomer 1 * 68000, SDS-PAGE Apis mellifera

Synonyms

EC Number Synonyms Comment Organism
3.2.1.20 alpha-glucosidase III
-
 Apis mellifera
3.2.1.20 HBG III
-
 Apis mellifera

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.20 40
-
 stable up to Apis mellifera
3.2.1.20 60
-
 15 min, complete inactivation Apis mellifera

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.20 additional information
-
 additional information
-
 Apis mellifera

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 5.5
-
-
 Apis mellifera

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.20 4.5
-
 labile below pH 4.5 Apis mellifera
3.2.1.20 5 10 stable Apis mellifera