BRENDA - Enzyme Database

Determination of urinary myo-inositol concentration by an improved enzymatic cycling method using myo-inositol dehydrogenase from Flavobacterium sp

Yamakoshi, M.; Takahashi, M.; Kouzuma, T.; Imamura, S.; Tsuboi, I.; Kawazu, S.; Yamagata, F.; Tominaga, M.; Noritake, M.; Clin. Chim. Acta 328, 163-171 (2003)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
1.1.1.18
analysis
determination of urinary myo-inositol by an improved enzymatic cycling method
Flavobacterium sp.
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.18
Flavobacterium sp.
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.18
D-chiro-inositol + NAD+
19% of the activity with myo-inositol
655247
Flavobacterium sp.
? + NADH
-
-
-
?
1.1.1.18
epi-inositol + NAD+
4% of the activity with myo-inositol
655247
Flavobacterium sp.
? + NADH
-
-
-
?
1.1.1.18
myo-inositol + NAD+
-
655247
Flavobacterium sp.
2,3,4,5,6-pentahydroxycyclohexanone + NADH
-
-
-
r
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.18
55
-
-
Flavobacterium sp.
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.18
45
-
30 min, 85% residual activity
Flavobacterium sp.
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.18
10
-
-
Flavobacterium sp.
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.1.18
5.5
8
-
Flavobacterium sp.
Application (protein specific)
EC Number
Application
Commentary
Organism
1.1.1.18
analysis
determination of urinary myo-inositol by an improved enzymatic cycling method
Flavobacterium sp.
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.18
D-chiro-inositol + NAD+
19% of the activity with myo-inositol
655247
Flavobacterium sp.
? + NADH
-
-
-
?
1.1.1.18
epi-inositol + NAD+
4% of the activity with myo-inositol
655247
Flavobacterium sp.
? + NADH
-
-
-
?
1.1.1.18
myo-inositol + NAD+
-
655247
Flavobacterium sp.
2,3,4,5,6-pentahydroxycyclohexanone + NADH
-
-
-
r
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.1.1.18
55
-
-
Flavobacterium sp.
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.18
45
-
30 min, 85% residual activity
Flavobacterium sp.
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.1.18
10
-
-
Flavobacterium sp.
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
1.1.1.18
5.5
8
-
Flavobacterium sp.