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Literature summary extracted from

  • Nasser, B.; El Kebbaj, M.S.; Cottin, P.; Latruffe, N.
    Purification and characterization of the D-beta-hydroxybutyrate dehydrogenase from dromedary liver mitochondria (2002), Comp. Biochem. Physiol. B, 131, 9-18.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.30 Phospholipids absolutely dependent on Camelus dromedarius

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.30 additional information
-
 additional information dissociation constants Camelus dromedarius
1.1.1.30 0.21
-
 NAD+ pH 8.1, 37°C Camelus dromedarius
1.1.1.30 0.27
-
 NADH pH 7.0, 37°C Camelus dromedarius
1.1.1.30 0.29
-
 acetoacetate pH 7.0, 37°C Camelus dromedarius
1.1.1.30 1.07
-
 (R)-3-hydroxybutyrate pH 8.1, 37°C Camelus dromedarius

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.30 membrane
-
 Camelus dromedarius 16020
-
1.1.1.30 mitochondrion
-
 Camelus dromedarius 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.30 67000
-
 x * 67000, SDS-PAGE Camelus dromedarius

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.30 Camelus dromedarius
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.30 about 1000fold, to homogeneity from liver mitochondrial membranes by solubilization with Triton X-100, DEAE-ion-exchange chromatography and phenyl-resin chromatography Camelus dromedarius

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.30 liver
-
 Camelus dromedarius
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.30 0.09
-
 purified enzyme Camelus dromedarius

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.30 (R)-3-hydroxybutyrate + NAD+
-
 Camelus dromedarius acetoacetate + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.30 ? x * 67000, SDS-PAGE Camelus dromedarius

Synonyms

EC Number Synonyms Comment Organism
1.1.1.30 BDH
-
 Camelus dromedarius
1.1.1.30 D-beta-hydroxybutyrate dehydrogenase
-
 Camelus dromedarius

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.30 37
-
-
 Camelus dromedarius

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.30 20 60 30% of maximal activity at 20°C, 63% of maximal activity at 60°C Camelus dromedarius

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.30
-
 37 stable at, 30% remaining activity after 40 min at 37°C, oxidation reaction Camelus dromedarius
1.1.1.30 45
-
 25% remaining activity after 20 min, oxidation reaction Camelus dromedarius
1.1.1.30 60
-
 28% remaining activity Camelus dromedarius

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.30 7
-
 approximately, reduction reaction Camelus dromedarius
1.1.1.30 8
-
 approximately, oxidation reaction Camelus dromedarius

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.30 NAD+
-
 Camelus dromedarius
1.1.1.30 NADH
-
 Camelus dromedarius