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Literature summary extracted from

  • Pontoppida, B.; Kannangara, C.G.
    Purification and partial characterization of barley glutamyl-tRNAGlu reductase,the enzyme that directs glutamate to chlorophyll biosynthesis (1994), Eur. J. Biochem., 225, 529-537.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.70 Cd2+ 1 mM Cd(Ac)2, 92% inhibition Hordeum vulgare
1.2.1.70 Co2+ 10 mM CoCl2, 78% inhibition Hordeum vulgare
1.2.1.70 Cu2+ 1 mM CuCl2, 84% inhibition Hordeum vulgare
1.2.1.70 Fe2+ 5 mM FeSO4, 66% inhibition Hordeum vulgare
1.2.1.70 Fe3+ 10 mM FeCl3, 80% inhibition Hordeum vulgare
1.2.1.70 glutamate 1-semialdehyde 0.2 mM, 50% inhibition Hordeum vulgare
1.2.1.70 heme 0.004 mM, 50% inhibition Hordeum vulgare
1.2.1.70 Zn2+ 2.5 mM, ZnSO4, 94% inhibition Hordeum vulgare

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.70 chloroplast stroma of greening chloroplast Hordeum vulgare 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.70 54000
-
 x * 54000, SDS-PAGE Hordeum vulgare
1.2.1.70 270000
-
 gel filtration Hordeum vulgare

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.70 L-glutamyl-tRNAGlu + NADPH + H+ Hordeum vulgare the enzyme directs glutamate to chlorophyll biosynthesis L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.70 Hordeum vulgare
-
 cultivar Svalöf Bonus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.70
-
 Hordeum vulgare

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.70 additional information
-
-
 Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.70 L-glutamyl-tRNAGlu + NADPH + H+
-
 Hordeum vulgare L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
 ?
1.2.1.70 L-glutamyl-tRNAGlu + NADPH + H+ the enzyme directs glutamate to chlorophyll biosynthesis Hordeum vulgare L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.70 ? x * 54000, SDS-PAGE Hordeum vulgare