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Literature summary extracted from

  • Camacho, M.; Rodriguez-Arnedo, A.; Bonete, M.J.
    NADP-dependent isocitrate dehydrogenase from the halophilic archaeon Haloferax volcanii: cloning, sequence determination and overexpression in Escherichia coli (2002), FEMS Microbiol. Lett., 209, 155-160.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.42 IDCH gene, DNA and amino acid sequence determination by combinated PCR and screening of genomic lambda EMBL3 library, and sequence analysis, overexpression in Escherichia coli BL21(DE3) as insoluble protein in inclusion bodies Haloferax volcanii

General Stability

EC Number General Stability Organism
1.1.1.42 3 M NaCl stabilizes the recombinant enzyme Haloferax volcanii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.42 0.093
-
 NADP+ recombinant, renatured enzyme, pH 8.0 Haloferax volcanii
1.1.1.42 0.105
-
 DL-isocitrate recombinant, renatured enzyme, pH 8.0 Haloferax volcanii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.42 Mg2+
-
 Haloferax volcanii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.42 45837
-
 x * 45837, sequence calculation, x * 60000, recombinant, renatured enzyme, SDS-PAGE Haloferax volcanii
1.1.1.42 60000
-
 x * 45837, sequence calculation, x * 60000, recombinant, renatured enzyme, SDS-PAGE Haloferax volcanii

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.42 Haloferax volcanii Q8X277 halophilic archaeon
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.42 recombinant solubilized enzyme by ultrafiltration, and DEAE ion exchange chromatography, 6.4fold Haloferax volcanii

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.1.1.42 solubilization in presence of 3 M NaCl of recombinant enzyme from inclusion bodies after expression in Escherichia coli Haloferax volcanii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.42 23.7
-
 purified recombinant enzyme Haloferax volcanii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.42 DL-isocitrate + NADP+
-
 Haloferax volcanii 2-oxoglutarate + CO2 + NADPH
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.42 ? x * 45837, sequence calculation, x * 60000, recombinant, renatured enzyme, SDS-PAGE Haloferax volcanii

Synonyms

EC Number Synonyms Comment Organism
1.1.1.42 ICDH
-
 Haloferax volcanii
1.1.1.42 NADP+-dependent Ds-threo-isocitrate dehydrogenase
-
 Haloferax volcanii
1.1.1.42 NADP+-dependent Ds-threo-isocitrate:NADP+ oxidoreductase
-
 Haloferax volcanii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.42 60
-
 recombinant enzyme, 93% remaining activity after 117 h, in presence of 3 M NaCl Haloferax volcanii
1.1.1.42 80
-
 recombinant enzyme, inactivation within 2.5 min, in presence of 3 M NaCl Haloferax volcanii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.42 8
-
 assay at Haloferax volcanii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.42 NADP+
-
 Haloferax volcanii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.1.1.42 Haloferax volcanii calculated
-
 4.9