EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.41 | gene icd, DNA and amino acid sequence determination and analysis, expression in enzyme-deficient Escherichia coli strain EB106 | Acidithiobacillus thiooxidans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.41 | additional information | - |
additional information | kinetic properties of the native and the recombinant enzyme are similar | Acidithiobacillus thiooxidans | |
1.1.1.41 | 0.18 | - |
NAD+ | pH 9.0, 30°C, native enzyme | Acidithiobacillus thiooxidans | |
1.1.1.41 | 6.57 | - |
NADP+ | pH 9.0, 30°C, native enzyme | Acidithiobacillus thiooxidans |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.41 | Mg2+ | - |
Acidithiobacillus thiooxidans |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.41 | 45000 | - |
2 * 45000, SDS-PAGE | Acidithiobacillus thiooxidans |
1.1.1.41 | 90000 | - |
gel filtration and native PAGE | Acidithiobacillus thiooxidans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.41 | isocitrate + NAD+ | Acidithiobacillus thiooxidans | - |
2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
1.1.1.41 | isocitrate + NAD+ | Acidithiobacillus thiooxidans ON107 | - |
2-oxoglutarate + CO2 + NADH + H+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.41 | Acidithiobacillus thiooxidans | Q8GAX0 | - |
- |
1.1.1.41 | Acidithiobacillus thiooxidans ON107 | Q8GAX0 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.41 | multi-step purification of the native enzyme, 520fold to homogeneity, partial purification of the recombinant enzyme from Escherichia coli | Acidithiobacillus thiooxidans |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.41 | 120 | - |
purified native enzyme | Acidithiobacillus thiooxidans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.41 | isocitrate + NAD+ | - |
Acidithiobacillus thiooxidans | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
1.1.1.41 | isocitrate + NAD+ | - |
Acidithiobacillus thiooxidans ON107 | 2-oxoglutarate + CO2 + NADH + H+ | - |
? | |
1.1.1.41 | isocitrate + NADP+ | - |
Acidithiobacillus thiooxidans | 2-oxoglutarate + CO2 + NADPH + H+ | - |
r | |
1.1.1.41 | isocitrate + NADP+ | - |
Acidithiobacillus thiooxidans ON107 | 2-oxoglutarate + CO2 + NADPH + H+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.41 | dimer | 2 * 45000, SDS-PAGE | Acidithiobacillus thiooxidans |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.41 | ICDH | - |
Acidithiobacillus thiooxidans |
1.1.1.41 | NAD+-dependent isocitrate dehydrogenase | - |
Acidithiobacillus thiooxidans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.41 | 30 | - |
assay at | Acidithiobacillus thiooxidans |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.41 | 55 | - |
stable up to | Acidithiobacillus thiooxidans |
1.1.1.41 | 70 | - |
inactivation after 30 min | Acidithiobacillus thiooxidans |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.41 | 21.3 | - |
NADP+ | pH 9.0, 30°C, native enzyme | Acidithiobacillus thiooxidans | |
1.1.1.41 | 45.1 | - |
NAD+ | pH 9.0, 30°C, native enzyme | Acidithiobacillus thiooxidans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.41 | 8.5 | - |
- |
Acidithiobacillus thiooxidans |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.41 | 6 | 10.2 | - |
Acidithiobacillus thiooxidans |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.41 | NAD+ | highly preferred cofactor, 78fold higher activity than with NADP+ | Acidithiobacillus thiooxidans | |
1.1.1.41 | NADP+ | 78fold lower activity than with NAD+ | Acidithiobacillus thiooxidans |