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Literature summary extracted from

  • Zhou, R.; Sicher, R.C.; Cheng, L.; Quebedeaux, B.
    Regulation of apple leaf aldose-6-phosphate reductase activity by inorganic phosphate and divalent cations (2003), Funct. Plant Biol., 30, 1037-1043.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.200 CaCl2 2.5 mM, in presence of 50 mM substrate, 12% decrease in activity Malus domestica
1.1.1.200 CuCl2
-
 Malus domestica
1.1.1.200 MgCl2 2.5 mM, in presence of 50 mM substrate, 12% decrease in activity Malus domestica
1.1.1.200 phosphate mixed-type inhibition Malus domestica
1.1.1.200 ZnCl2 2 mM, 58% inhibition, partially reversible by EDTA Malus domestica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.200 1.7
-
 D-sorbitol-6-phosphate pH 9.0 Malus domestica
1.1.1.200 3
-
 D-sorbitol-6-phosphate pH 9.0 Malus domestica
1.1.1.200 5.1
-
 D-Glucose-6-phosphate pH 9.0, presence of 2.5 mM MgCl2 Malus domestica
1.1.1.200 9.8
-
 D-Glucose-6-phosphate pH 9.0 Malus domestica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.200 CaCl2 2.5 mM, in presence of 5 mM substrate, 40% increase in activity Malus domestica
1.1.1.200 MgCl2 2.5 mM, in presence of 5 mM substrate, 52% increase in activity Malus domestica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.200 33000
-
 2 * 33000, SDS-PAGE Malus domestica
1.1.1.200 68000
-
 gel filtration Malus domestica

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.200 Malus domestica
-
 Borkh. cv. Gala
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.200
-
 Malus domestica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.200 leaf fully developed Malus domestica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.200 7.94
-
 pH 9.0 Malus domestica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.200 D-glucose 6-phosphate + NADPH
-
 Malus domestica D-sorbitol 6-phosphate + NADP+
-
 r
1.1.1.200 D-sorbitol 6-phosphate + NADP+
-
 Malus domestica D-glucose 6-phosphate + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.200 dimer 2 * 33000, SDS-PAGE Malus domestica

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.200 NADP+
-
 Malus domestica
1.1.1.200 NADPH
-
 Malus domestica

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.200 0.42
-
 phosphate pH 9.0 Malus domestica