EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.40 | Cu2+-ascorbate | rapid inactivation by generation of reactive oxygen species at pH 5.0, Fe2+ can substitute for Cu2+, Cu2+ or ascorbate alone are not effective, azide, 1,4-diazabicyclo-(2.2.2.)octane, histidine and imidazole protect against inhibition, the substrates L-malate and NADP+ and EDTA protect almost completely, loss of activity is accompanied with cleavage of the protein into 4 fragments of 14-55 kDa | Zea mays | |
1.1.1.40 | Fe2+-ascorbate | rapid inactivation | Zea mays | |
1.1.1.40 | H2O2 | 83% inhibition at 0.25 mM, 91.1% inhibition at 0.5 mM | Zea mays |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.40 | Mg2+ | - |
Zea mays |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.40 | 62000 | - |
4 * 62000, SDS-PAGE | Zea mays |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.40 | Zea mays | - |
- |
- |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.40 | leaf | - |
Zea mays | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.40 | (S)-malate + NADP+ | - |
Zea mays | pyruvate + CO2 + NADPH | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.40 | tetramer | 4 * 62000, SDS-PAGE | Zea mays |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.40 | NADP-malic enzyme | - |
Zea mays |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.40 | 25 | - |
assay at | Zea mays |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.40 | 7.5 | - |
assay at | Zea mays |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.40 | NADP+ | - |
Zea mays |