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Literature summary extracted from
- Vacuolar type H+ pumping pyrophosphatases of parasitic protozoa (2002), Int. J. Parasitol., 32, 1-14.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.3.1 | K+ | stimulates activity | Trypanosoma cruzi |  |
| 7.1.3.1 | K+ | stimulates activity | Plasmodium falciparum | |
| 7.1.3.1 | K+ | stimulates activity | Thermotoga maritima | |
| 7.1.3.1 | K+ | stimulates activity | Toxoplasma gondii | |
| 7.1.3.1 | additional information | insensitive to stimulation with K+ | Arabidopsis thaliana |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 7.1.3.1 | E263D | 50% of hydrolytic activity, no protein translocation | Vigna radiata |
| 7.1.3.1 | K261R | 25% of hydrolytic activity, no protein translocation | Vigna radiata |
| 7.1.3.1 | V259A | 60% of hydrolytic activity, no protein translocation | Vigna radiata |
| 7.1.3.1 | V262A | no effect on activity | Vigna radiata |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 7.1.3.1 | Mg2+ protects against inactivation and protease digestion | Rhodospirillum rubrum |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.3.1 | bisphosphonates | weak inhibition | Plasmodium falciparum | |
| 7.1.3.1 | bisphosphonates | - |
Toxoplasma gondii | |
| 7.1.3.1 | bisphosphonates | - |
Trypanosoma cruzi | |
| 7.1.3.1 | risedronate | IC50: 0.123 mM | Plasmodium falciparum | |
| 7.1.3.1 | risedronate | IC50: 0.002 mM | Toxoplasma gondii | |
| 7.1.3.1 | risedronate | IC50: 0.123 mM | Trypanosoma cruzi | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 7.1.3.1 | acidocalcisome | - |
Trypanosoma cruzi | 20022 | - |
| 7.1.3.1 | membrane | - |
Plasmodium falciparum | 16020 | - |
| 7.1.3.1 | membrane | - |
Rhodospirillum rubrum | 16020 | - |
| 7.1.3.1 | membrane | - |
Thermotoga maritima | 16020 | - |
| 7.1.3.1 | membrane | - |
Pyrobaculum aerophilum | 16020 | - |
| 7.1.3.1 | plasma membrane | - |
Trypanosoma cruzi | 5886 | - |
| 7.1.3.1 | plasma membrane | - |
Toxoplasma gondii | 5886 | - |
| 7.1.3.1 | vacuolar membrane | - |
Zea mays | 5774 | - |
| 7.1.3.1 | vacuolar membrane | - |
Arabidopsis thaliana | 5774 | - |
| 7.1.3.1 | vacuolar membrane | - |
Vigna radiata | 5774 | - |
| 7.1.3.1 | vacuolar membrane | - |
Toxoplasma gondii | 5774 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 7.1.3.1 | Mg2+ | required | Zea mays | |
| 7.1.3.1 | Mg2+ | required | Arabidopsis thaliana | |
| 7.1.3.1 | Mg2+ | required | Trypanosoma cruzi | |
| 7.1.3.1 | Mg2+ | required | Vigna radiata | |
| 7.1.3.1 | Mg2+ | required | Plasmodium falciparum | |
| 7.1.3.1 | Mg2+ | required | Rhodospirillum rubrum | |
| 7.1.3.1 | Mg2+ | required | Thermotoga maritima | |
| 7.1.3.1 | Mg2+ | required | Pyrobaculum aerophilum | |
| 7.1.3.1 | Mg2+ | required | Toxoplasma gondii | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.1.3.1 | diphosphate + H2O + H+[side 1] | Zea mays | important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy | 2 phosphate + H+[side 2] | - |
? | |
| 7.1.3.1 | diphosphate + H2O + H+[side 1] | Arabidopsis thaliana | important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy | 2 phosphate + H+[side 2] | - |
? | |
| 7.1.3.1 | diphosphate + H2O + H+[side 1] | Trypanosoma cruzi | important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy | 2 phosphate + H+[side 2] | - |
? | |
| 7.1.3.1 | diphosphate + H2O + H+[side 1] | Vigna radiata | important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy | 2 phosphate + H+[side 2] | - |
? | |
| 7.1.3.1 | diphosphate + H2O + H+[side 1] | Plasmodium falciparum | important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy | 2 phosphate + H+[side 2] | - |
? | |
| 7.1.3.1 | diphosphate + H2O + H+[side 1] | Rhodospirillum rubrum | important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy | 2 phosphate + H+[side 2] | - |
? | |
| 7.1.3.1 | diphosphate + H2O + H+[side 1] | Thermotoga maritima | important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy | 2 phosphate + H+[side 2] | - |
? | |
| 7.1.3.1 | diphosphate + H2O + H+[side 1] | Pyrobaculum aerophilum | important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy | 2 phosphate + H+[side 2] | - |
? | |
| 7.1.3.1 | diphosphate + H2O + H+[side 1] | Toxoplasma gondii | important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy | 2 phosphate + H+[side 2] | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 7.1.3.1 | Arabidopsis thaliana | - |
- |
- |
| 7.1.3.1 | Plasmodium falciparum | - |
hyperthermophilic bacterium | - |
| 7.1.3.1 | Pyrobaculum aerophilum | - |
hyperthermophilic bacterium | - |
| 7.1.3.1 | Rhodospirillum rubrum | - |
- |
- |
| 7.1.3.1 | Thermotoga maritima | - |
hyperthermophilic bacterium | - |
| 7.1.3.1 | Toxoplasma gondii | - |
- |
- |
| 7.1.3.1 | Trypanosoma cruzi | - |
hyperthermophilic bacterium | - |
| 7.1.3.1 | Vigna radiata | - |
mung bean | - |
| 7.1.3.1 | Zea mays | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 7.1.3.1 | trophozoite | - |
Plasmodium falciparum | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 7.1.3.1 | diphosphate + H2O + H+[side 1] | - |
Zea mays | 2 phosphate + H+[side 2] | - |
? | |
| 7.1.3.1 | diphosphate + H2O + H+[side 1] | - |
Arabidopsis thaliana | 2 phosphate + H+[side 2] | - |
? | |
| 7.1.3.1 | diphosphate + H2O + H+[side 1] | - |
Trypanosoma cruzi | 2 phosphate + H+[side 2] | - |
? | |
| 7.1.3.1 | diphosphate + H2O + H+[side 1] | - |
Vigna radiata | 2 phosphate + H+[side 2] | - |
? | |
| 7.1.3.1 | diphosphate + H2O + H+[side 1] | - |
Plasmodium falciparum | 2 phosphate + H+[side 2] | - |
? | |
| 7.1.3.1 | diphosphate + H2O + H+[side 1] | - |
Rhodospirillum rubrum | 2 phosphate + H+[side 2] | - |
r | |
| 7.1.3.1 | diphosphate + H2O + H+[side 1] | - |
Thermotoga maritima | 2 phosphate + H+[side 2] | - |
? | |
| 7.1.3.1 | diphosphate + H2O + H+[side 1] | - |
Pyrobaculum aerophilum | 2 phosphate + H+[side 2] | - |
? | |
| 7.1.3.1 | diphosphate + H2O + H+[side 1] | - |
Toxoplasma gondii | 2 phosphate + H+[side 2] | - |
? | |
| 7.1.3.1 | diphosphate + H2O + H+[side 1] | important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy | Zea mays | 2 phosphate + H+[side 2] | - |
? | |
| 7.1.3.1 | diphosphate + H2O + H+[side 1] | important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy | Arabidopsis thaliana | 2 phosphate + H+[side 2] | - |
? | |
| 7.1.3.1 | diphosphate + H2O + H+[side 1] | important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy | Trypanosoma cruzi | 2 phosphate + H+[side 2] | - |
? | |
| 7.1.3.1 | diphosphate + H2O + H+[side 1] | important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy | Vigna radiata | 2 phosphate + H+[side 2] | - |
? | |
| 7.1.3.1 | diphosphate + H2O + H+[side 1] | important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy | Plasmodium falciparum | 2 phosphate + H+[side 2] | - |
? | |
| 7.1.3.1 | diphosphate + H2O + H+[side 1] | important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy | Rhodospirillum rubrum | 2 phosphate + H+[side 2] | - |
? | |
| 7.1.3.1 | diphosphate + H2O + H+[side 1] | important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy | Thermotoga maritima | 2 phosphate + H+[side 2] | - |
? | |
| 7.1.3.1 | diphosphate + H2O + H+[side 1] | important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy | Pyrobaculum aerophilum | 2 phosphate + H+[side 2] | - |
? | |
| 7.1.3.1 | diphosphate + H2O + H+[side 1] | important for energy metabolism, provides energy for biosynthetic reactions, proton translocation through membrane provides a source of energy | Toxoplasma gondii | 2 phosphate + H+[side 2] | - |
? | |
IC50 Value
| EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|---|
| 7.1.3.1 | 0.002 | - |
IC50: 0.002 mM | Toxoplasma gondii | risedronate | |
| 7.1.3.1 | 0.123 | - |
IC50: 0.123 mM | Trypanosoma cruzi | risedronate | |
| 7.1.3.1 | 0.123 | - |
IC50: 0.123 mM | Plasmodium falciparum | risedronate | |
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