EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.91 | - |
Trichoderma reesei |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.2.1.91 | - |
Trichoderma reesei |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.91 | D175A | mutant retains some activity on longer oligosaccharides | Trichoderma reesei |
3.2.1.91 | D221A | almost complete loss of activity | Trichoderma reesei |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.91 | 1.3 | - |
cellopentaose | pH 5, 27°C, wild-type enzyme | Trichoderma reesei | |
3.2.1.91 | 1.4 | - |
cellopentaose | pH 5, 27°C, D175A mutant | Trichoderma reesei | |
3.2.1.91 | 2.6 | - |
cellotetraose | pH 5, 27°C, wild-type enzyme | Trichoderma reesei | |
3.2.1.91 | 4 | - |
cellotetraose | pH 5, 27°C, D175A mutant | Trichoderma reesei | |
3.2.1.91 | 14 | - |
cellohexaose | pH 5, 27°C, wild-type enzyme | Trichoderma reesei | |
3.2.1.91 | 17 | - |
cellotriose | pH 5, 27°C, wild-type enzyme | Trichoderma reesei |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.91 | Trichoderma reesei | P07987 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.91 | cellohexaose + H2O | - |
Trichoderma reesei | ? | - |
? | |
3.2.1.91 | cellopentaose + H2O | - |
Trichoderma reesei | ? | - |
? | |
3.2.1.91 | cellotetraose + H2O | - |
Trichoderma reesei | ? | - |
? | |
3.2.1.91 | cellotriose + H2O | - |
Trichoderma reesei | ? | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.91 | 0.0003 | - |
cellotriose | pH 5, 27°C, D175A mutant | Trichoderma reesei | |
3.2.1.91 | 0.0013 | - |
cellotetraose | pH 5, 27°C, D175A mutant | Trichoderma reesei | |
3.2.1.91 | 0.02 | - |
cellohexaose | pH 5, 27°C, D175A mutant | Trichoderma reesei | |
3.2.1.91 | 0.025 | - |
cellopentaose | pH 5, 27°C, D175A mutant | Trichoderma reesei | |
3.2.1.91 | 0.06 | - |
cellotriose | pH 5, 27°C, wild-type enzyme | Trichoderma reesei | |
3.2.1.91 | 1.1 | - |
cellopentaose | pH 5, 27°C, wild-type enzyme | Trichoderma reesei | |
3.2.1.91 | 4.1 | - |
cellotetraose | pH 5, 27°C, wild-type enzyme | Trichoderma reesei | |
3.2.1.91 | 14 | - |
cellohexaose | pH 5, 27°C, wild-type enzyme | Trichoderma reesei |