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Literature summary extracted from

  • Gao, G.J.; Fonda, M.L.
    Kinetic analysis and chemical modification of vitamin B6 phosphatase from human erythrocytes (1994), J. Biol. Chem., 269, 7163-7168.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.74 5,5'-dithiobis(2-nitrobenzoate)
-
 Homo sapiens
3.1.3.74 diethyldicarbonate inactivates by reacting with a group with a pKalpha of 6.7, kinetics, pyridoxine 5’-phosphate protects, 100 mM neutral hydroxylamine partially reactivates Homo sapiens
3.1.3.74 N-(5'-phospho-4'-pyridoxyl)ethanolamine 0.05 mM, 12% inhibition Homo sapiens
3.1.3.74 N-(5'-phospho-4'-pyridoxyl)glycine 0.05 mM, 32% inhibition Homo sapiens
3.1.3.74 N-(5'-phospho-4'-pyridoxyl)phenylalanine 0.05 mM, 51% inhibition Homo sapiens
3.1.3.74 Phenylglyoxal the incorporation of 1 mol per subunit inactivates, pyridoxal 5’-phosphate protects, kinetics Homo sapiens
3.1.3.74 pyridoxine 5'-phosphate 0.05 mM, 45% inhibition Homo sapiens
3.1.3.74 Tetranitromethane inactivates in a time-dependent manner, 10 mM, 70% inhibition in the absence of pyridoxal 5’-phosphate and 30% in the presence of 0.15 mM pyridoxal 5’-phosphate Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.74 additional information
-
 additional information kinetic data, effect of pH on Km for 4-pyridoxic acid 5’-phosphate, pyridoxal 5’-phosphate, pyridoxine 5’-phosphate and pyridoxamine 5’-phosphate Homo sapiens
3.1.3.74 0.00112
-
 4-pyridoxic acid 5'-phosphate pH 7.4, 37°C Homo sapiens
3.1.3.74 0.00129
-
 pyridoxal 5'-phosphate pH 7.4, 37°C Homo sapiens
3.1.3.74 0.00824
-
 N-(5'-phospho-4'-pyridoxyl)phenylalanine pH 7.4, 37°C Homo sapiens
3.1.3.74 0.0106
-
 pyridoxine 5'-phosphate pH 7.4, 37°C Homo sapiens
3.1.3.74 0.0143
-
 N-(5'-phospho-4'-pyridoxyl)glycine pH 7.4, 37°C Homo sapiens
3.1.3.74 0.0547
-
 pyridoxamine 5'-phosphate pH 7.4, 37°C Homo sapiens
3.1.3.74 0.0635
-
 N-(5'-phospho-4'-pyridoxyl)benzylamine pH 7.4, 37°C Homo sapiens
3.1.3.74 0.0786
-
 N-(5'-phospho-4'-pyridoxyl)ethanolamine pH 7.4, 37°C Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.74 Mg2+ requirement Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.74 64000
-
-
 Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.74 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.74 168000fold Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.3.74 erythrocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.74 1.4
-
 pH 7.4, 37°C Homo sapiens

Storage Stability

EC Number Storage Stability Organism
3.1.3.74 4°C, storage at concentrations of 0.3 mg/ml or greater, 30 mM phosphate, at least 6 months, stable Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.74 4-pyridoxic acid 5'-phosphate + H2O highest catalytic efficiency with 4-pyridoxic acid 5’-phosphate and pyridoxal 5’-phosphate Homo sapiens 4-pyridoxic acid + phosphate
-
 ?
3.1.3.74 additional information specificity and active site properties, enzyme also catalyzes the dephosphorylation of 4’-secondary amine derivatives of vitamin B6 phosphate, enzyme has the greatest catalytic efficiency with substrates that contain a negatively charged group on the 4’-position of the pyridine ring, one or two positively charged groups at the active site of enzyme interacts with the substrate’s phosphate ester and 4’-substituent, Arg and His residues are at or near the active site and may play roles in substrate binding and/or catalysis, very low activity with p-nitrophenylphosphate Homo sapiens ?
-
 ?
3.1.3.74 N-(5'-phospho-4'-pyridoxyl)benzylamine + H2O
-
 Homo sapiens 4'-pyridoxylbenzylamine + phosphate
-
 ?
3.1.3.74 N-(5'-phospho-4'-pyridoxyl)ethanolamine + H2O
-
 Homo sapiens 4'-pyridoxylethanolamine + phosphate
-
 ?
3.1.3.74 N-(5'-phospho-4'-pyridoxyl)glycine + H2O
-
 Homo sapiens 4'-pyridoxylglycine + phosphate
-
 ?
3.1.3.74 N-(5'-phospho-4'-pyridoxyl)phenylalanine + H2O much higher catalytic efficiency than with pyridoxine 5’-phosphate Homo sapiens 4'-pyridoxylphenylalanine + phosphate
-
 ?
3.1.3.74 pyridoxal 5'-phosphate + H2O highest catalytic efficiency with pyridoxal 5’-phosphate and 4-pyridoxic acid 5’-phosphate Homo sapiens pyridoxal + phosphate
-
 ?
3.1.3.74 pyridoxamine 5'-phosphate + H2O low hydrolysis rate Homo sapiens pyridoxamine + phosphate
-
 ?
3.1.3.74 pyridoxine 5'-phosphate + H2O
-
 Homo sapiens pyridoxine + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.74 dimer
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.74 22
-
 assay at, inhibition studies Homo sapiens
3.1.3.74 37
-
 assay at, kinetic analysis Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.74 6 8
-
 Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.74 5 9 60 min, no loss of activity Homo sapiens