Literature summary extracted from
Lin, A.P.; McAlister-Henn, L.
Homologous binding sites in yeast isocitrate dehydrogenase for cofactor (NAD+) and allosteric activator (AMP) (2003), J. Biol. Chem., 278, 12864-12872.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
1.1.1.41 |
AMP |
allosteric activator, binding site at subunit IDH1 |
Saccharomyces cerevisiae |
|
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.1.1.41 |
expression of His-tagged wild-type and mutant enzymes in yeast strain IDH12DELTAL deficient in both subunits of the enzyme |
Saccharomyces cerevisiae |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
1.1.1.41 |
D279A/D280A |
site-directed mutagenesis, mutation of a IDH1 residue, reduced AMP binding, ligand-binding analysis |
Saccharomyces cerevisiae |
1.1.1.41 |
D286A/I287A |
site-directed mutagenesis, mutation of a IDH2 residue, highly reduced NAD+ binding, ligand-binding analysis |
Saccharomyces cerevisiae |
1.1.1.41 |
H281A |
site-directed mutagenesis, mutation of a IDH2 residue, mutant cannot bind NAD+, ligand-binding analysis |
Saccharomyces cerevisiae |
1.1.1.41 |
R274A |
site-directed mutagenesis, mutation of a IDH1 residue, reduced AMP binding, ligand-binding analysis |
Saccharomyces cerevisiae |
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
1.1.1.41 |
additional information |
- |
additional information |
kinetics and ligand binding |
Saccharomyces cerevisiae |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
1.1.1.41 |
mitochondrion |
- |
Saccharomyces cerevisiae |
5739 |
- |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.1.1.41 |
Mg2+ |
binding site at subunit IDH2, active site |
Saccharomyces cerevisiae |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
1.1.1.41 |
303000 |
- |
sequence calculation |
Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
1.1.1.41 |
isocitrate + NAD+ |
Saccharomyces cerevisiae |
enzyme performs the rate-limiting step in the mitochondrial tricarboxylic cycle and is subject to complex allosteric regulation |
2-oxoglutarate + CO2 + NADH + H+ |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.1.1.41 |
Saccharomyces cerevisiae |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.1.1.41 |
recombinant His-tagged wild-type and mutant enzymes from strain IDH12DELTAL by nickel affinity chromatography |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.1.1.41 |
D-isocitrate + NAD+ |
catalytic residues binding isocitrate are located on subunit IDH2, while regulatory residues binding isocitrate are located on subunit IDH1 |
Saccharomyces cerevisiae |
2-oxoglutarate + CO2 + NADH |
- |
? |
|
1.1.1.41 |
isocitrate + NAD+ |
enzyme performs the rate-limiting step in the mitochondrial tricarboxylic cycle and is subject to complex allosteric regulation |
Saccharomyces cerevisiae |
2-oxoglutarate + CO2 + NADH + H+ |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.1.1.41 |
octamer |
alpha4beta4, subunits are termed IDH1 and IDH2 |
Saccharomyces cerevisiae |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.1.1.41 |
IDH |
- |
Saccharomyces cerevisiae |
1.1.1.41 |
isocitrate dehydrogenase |
- |
Saccharomyces cerevisiae |
1.1.1.41 |
NAD+-specific isocitrate dehydrogenase |
- |
Saccharomyces cerevisiae |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
1.1.1.41 |
24 |
- |
assay at |
Saccharomyces cerevisiae |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
1.1.1.41 |
7.4 |
- |
assay at |
Saccharomyces cerevisiae |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.1.1.41 |
NAD+ |
specific for, binding site at subunit IDH2 |
Saccharomyces cerevisiae |
|