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Literature summary extracted from

  • Cobucci-Ponzano, B.; Trincone, A.; Giordano, A.; Rossi, M.; Moracci, M.
    Identification of an archaeal alpha-L-fucosidase encoded by an interrupted gene. Production of a functional enzyme by mutations mimicking programmed -1 frameshifting (2003), J. Biol. Chem., 278, 14622-14631.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.51 expression in Escherichia coli Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.51 0.028
-
 p-nitrophenyl alpha-L-fucoside pH 6.3, 65°C Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.51 57000
-
 10 * 57000, recombinant enzyme Saccharolobus solfataricus
3.2.1.51 508000
-
 gel filtration, recombinant enzyme Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.51 Saccharolobus solfataricus
-
 P2 and MT4
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.51 32.1
-
 recombinant enzyme Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.51 additional information Two specific mutations, designed on the basis of the programmed -1 frameshifting mechanism, promotes transfucosylation reactions by following a retaining reaction mechanism. Saccharolobus solfataricus ?
-
 ?
3.2.1.51 p-nitrophenyl alpha-L-fucoside + H2O fase II, not: fase I Saccharolobus solfataricus p-nitrophenol + alpha-L-fucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.51 decamer 10 * 57000, recombinant enzyme Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.51 95
-
 the activity increases sharply up to the optimal temperature of 95°C, the highest temperature tested Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.51 75
-
 high stability Saccharolobus solfataricus
3.2.1.51 80
-
 60% residual activity after 2 h Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.51 287
-
 p-nitrophenyl alpha-L-fucoside pH 6.3, 65°C Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.51 3.3 6.3 65°C Saccharolobus solfataricus