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Literature summary extracted from

  • Lee, R.C.; Hrmova, M.; Burton, R.A.; Lahnstein, J.; Fincher, G.B.
    Bifunctional family of 3 glycoside hydrolases from barley with alpha-L-arabinofuranosidas and beta-D-xylosidase activity (2003), J. Biol. Chem., 278, 5377-5387.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.55 DNA and amino acid sequence determination and analysis, encoded on chromosome 2H Hordeum vulgare

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.55 67000
-
 x * 67000, SDS-PAGE, x * 79200, about, amino acid sequence calculation Hordeum vulgare
3.2.1.55 79200
-
 x * 67000, SDS-PAGE, x * 79200, about, amino acid sequence calculation Hordeum vulgare

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.55 Hordeum vulgare Q8W012 bifunctional enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.55 1080fold from 5 days old seedlings Hordeum vulgare

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.55 1,5-alpha-L-arabinofuranohexaose + 5 H2O = 6 alpha-L-arabinofuranose enzyme shows 2 catalytic activities: alpha-L-arabinofuranosidase and beta-D-xylosidase activity Hordeum vulgare
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.55 leaf young Hordeum vulgare
-
3.2.1.55 root young Hordeum vulgare
-
3.2.1.55 seedling
-
 Hordeum vulgare
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.55 6610
-
 purified enzyme Hordeum vulgare

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.55 (1-4)-beta-D-xylopentaose + H2O
-
 Hordeum vulgare beta-D-xylose
-
 ?
3.2.1.55 (1-5)-alpha-L-arabinofuranohexaose + H2O
-
 Hordeum vulgare alpha-L-arabinofuranose
-
 ?
3.2.1.55 4-nitrophenyl alpha-L-arabinofuranoside + H2O best substrate Hordeum vulgare 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.55 4-nitrophenyl beta-D-galactopyranoside + H2O 16% activity compared to 4-nitrophenyl-alpha-L-arabinofuranoside Hordeum vulgare 4-nitrophenol + beta-D-galactopyranose
-
 ?
3.2.1.55 4-nitrophenyl beta-D-xylopyranoside + H2O 20% activity compared to 4-nitrophenyl-alpha-L-arabinofuranoside Hordeum vulgare 4-nitrophenol + beta-D-xylopyranose
-
 ?
3.2.1.55 4-nitrophenyl-alpha-L-arabinopyranoside + H2O 11% activity compared to 4-nitrophenyl-alpha-L-arabinofuranoside Hordeum vulgare 4-nitrophenol + alpha-L-arabinopyranose
-
 ?
3.2.1.55 additional information stereospecific, enzyme shows 2 catalytic activities: alpha-L-arabinofuranosidase and beta-D-xylosidase activity, no activity with 4-nitrophenyl-beta-D-glucopyranoside, 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, 4-nitrophenyl-beta-D-mannopyranoside, 4-nitrophenyl-alpha-D-mannopyranoside, 4-nitrophenyl-alpha-D-xylopyranoside, 4-nitrophenyl-beta-L-arabinopyranoside Hordeum vulgare ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.55 ? x * 67000, SDS-PAGE, x * 79200, about, amino acid sequence calculation Hordeum vulgare

Synonyms

EC Number Synonyms Comment Organism
3.2.1.55 alpha-L-arabinofuranosidase
-
 Hordeum vulgare
3.2.1.55 ARA-I
-
 Hordeum vulgare
3.2.1.55 More enzyme belongs to family 3 of glycoside hydrolases Hordeum vulgare

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.55 4.7
-
-
 Hordeum vulgare

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.55 Hordeum vulgare isoelectric focusing
-
 5.5