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Literature summary extracted from

  • Hijarrubia, M.J.; Aparicio, J.F.; Martin, J.F.
    Domain structure characterization of the multifunctional alpha-aminoadipate reductase from Penicillium chrysogenum by limited proteolysis. Activation of alpha-aminoadipate does not require the peptidyl carrier protein box or the reduction domain (2003), J. Biol. Chem., 278, 8250-8256.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.31 ATP presence of ATP required Penicillium chrysogenum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.31 EDTA 5 mM, 75% inhibition Penicillium chrysogenum
1.2.1.95 EDTA 5 mM, 75% inhibition Penicillium chrysogenum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.31 0.16
-
 NADPH pH 8, 30°C Penicillium chrysogenum
1.2.1.31 1.3
-
 ATP pH 8, 30°C Penicillium chrysogenum
1.2.1.31 1.4
-
 L-2-aminoadipate pH 8, 30°C Penicillium chrysogenum
1.2.1.95 0.16
-
 NADPH pH 9.0, 30°C Penicillium chrysogenum
1.2.1.95 1.3
-
 ATP pH 9.0, 30°C Penicillium chrysogenum
1.2.1.95 1.4
-
 L-2-aminoadipate pH 9.0, 30°C Penicillium chrysogenum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.31 Mg2+ absolue requiremnt, optimal concentration: 10 mM Penicillium chrysogenum
1.2.1.31 Mn2+ optimal concentration: 5 mM, 60% of the maximal activity attained with Mg2+ Penicillium chrysogenum
1.2.1.95 Mg2+ absolutely required for activity, optimal concentration 10 mM Penicillium chrysogenum
1.2.1.95 Mn2+ can partly replace Mg2+, leading to 60% of maximum activity with Mg2+ Penicillium chrysogenum
1.2.1.95 additional information no activity with Ca2+, Co2+, Hg2+, Cu2+ Penicillium chrysogenum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.31 155000
-
 1 * 155000, SDS-PAGE Penicillium chrysogenum
1.2.1.31 171000
-
 gel filtration Penicillium chrysogenum
1.2.1.95 154800
-
 1 * 155000, SDS-PAGE, 1 * 154800, calculated Penicillium chrysogenum
1.2.1.95 155000
-
 1 * 155000, SDS-PAGE, 1 * 154800, calculated Penicillium chrysogenum
1.2.1.95 171000
-
 gel filtration Penicillium chrysogenum

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.31 Penicillium chrysogenum
-
-
-
1.2.1.95 Penicillium chrysogenum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.31
-
 Penicillium chrysogenum
1.2.1.95
-
 Penicillium chrysogenum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.31 additional information
-
-
 Penicillium chrysogenum

Storage Stability

EC Number Storage Stability Organism
1.2.1.31 -20°C, 50% v/v glycerol, rapid loss of activity Penicillium chrysogenum
1.2.1.95 -20°C, stable Penicillium chrysogenum
1.2.1.95 4°C, stable Penicillium chrysogenum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.31 L-2-aminoadipate + NADPH + H+ presence of ATP required Penicillium chrysogenum L-2-aminoadipate 6-semialdehyde + NADP+ + H2O
-
 ?
1.2.1.31 S-carboxymethyl-L-cysteine + NADPH 5% of the activity with L-2-aminoadipate Penicillium chrysogenum ?
-
 ?
1.2.1.95 L-2-aminoadipate + NADPH + H+ + ATP
-
 Penicillium chrysogenum (S)-2-amino-6-oxohexanoate + NADP+ + AMP + diphosphate
-
 ?
1.2.1.95 additional information no substrates: adipic acid, DL-diaminopimelic acid, L-glutamate Penicillium chrysogenum ?
-
 ?
1.2.1.95 S-carboxymethyl-L-cysteine + NADPH + H+ + ATP 20fold lower activity than with L-2-aminoadipate Penicillium chrysogenum ? + NADP+ + AMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.31 monomer 1 * 155000, SDS-PAGE Penicillium chrysogenum
1.2.1.95 monomer 1 * 155000, SDS-PAGE, 1 * 154800, calculated Penicillium chrysogenum

Synonyms

EC Number Synonyms Comment Organism
1.2.1.95 alpha-AAR
-
 Penicillium chrysogenum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.31 25 29
-
 Penicillium chrysogenum
1.2.1.95 25 29
-
 Penicillium chrysogenum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.2.1.95 30
-
 rapid loss of activity above Penicillium chrysogenum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.2.1.31 4
-
 1 h, stable Penicillium chrysogenum
1.2.1.31 30
-
 1 h, 20% loss of activity Penicillium chrysogenum
1.2.1.31 37
-
 15 min, 50% loss of activity Penicillium chrysogenum
1.2.1.95 30
-
 1h, 20% loss of activity Penicillium chrysogenum
1.2.1.95 37
-
 15 min, 50% loss of activity Penicillium chrysogenum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.31 66
-
 L-2-aminoadipate pH 8, 30°C Penicillium chrysogenum
1.2.1.95 1.1
-
 L-2-aminoadipate pH 9.0, 30°C Penicillium chrysogenum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.31 9
-
-
 Penicillium chrysogenum
1.2.1.95 9
-
-
 Penicillium chrysogenum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.95 7.5 9.5
-
 Penicillium chrysogenum

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.31 NADPH
-
 Penicillium chrysogenum