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Literature summary extracted from

  • Koh, H.J.; Lee, S.M.; Son, B.G.; Lee, S.H.; Ryoo, Z.Y.; Chang, K.T.; Park, J.W.; Park, D.C.; Song, B.J.; Veech, R.L.; Song, H.; Huh, T.L.
    Cytosolic NADP+-dependent isocitrate dehydrogenase plays a key role in lipid metabolism (2004), J. Biol. Chem., 279, 39968-39974.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.42 ADP
-
 Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.42 expression of the cDNA in a construct of nucleotides 1-1714 with rat PEPCK promotor and the SV-40 polyadenylation signal, permanant expression in 3T3-L1 cells and in transgenic mice, the latter are constructed by infection of fertilized eggs Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.42 additional information construction of transgenic mice by infection of fertilized eggs, enzyme overexpressing transgenic mice show increased triglyceride and cholesterol levels and develop fatty liver, hyperlipidemia, and obesity Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.42 cytosol
-
 Mus musculus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.42 Mg2+
-
 Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.42 isocitrate + NADP+ Mus musculus cytosolic enzyme is critical in fat and cholesterol biosynthesis, enzyme content correlates with adipogenesis in wild-type adipocytes and in transgenic cells 2-oxoglutarate + CO2 + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.42 Mus musculus
-
 26-weeks-old male mice
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.42 adipocyte
-
 Mus musculus
-
1.1.1.42 adipose tissue
-
 Mus musculus
-
1.1.1.42 epididymis epididymal fat pad Mus musculus
-
1.1.1.42 fat pad epididymal fat pad, hepatic fat pad Mus musculus
-
1.1.1.42 liver
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.42 18.6
-
 wild-type epidydymal fat pad Mus musculus
1.1.1.42 50
-
 transgenic mice epidydymal fat pad Mus musculus
1.1.1.42 77.4
-
 wild-type liver Mus musculus
1.1.1.42 108.2
-
 transgenic mice liver Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.42 isocitrate + NADP+ cytosolic enzyme is critical in fat and cholesterol biosynthesis, enzyme content correlates with adipogenesis in wild-type adipocytes and in transgenic cells Mus musculus 2-oxoglutarate + CO2 + NADPH + H+
-
 ?
1.1.1.42 threo-Ds-isocitrate + NADP+
-
 Mus musculus 2-oxoglutarate + CO2 + NADPH
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.42 IDPc
-
 Mus musculus
1.1.1.42 NADP+-dependent isocitrate dehydrogenase
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.42 25
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.42 7.2
-
 assay at Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.42 NADP+ essential Mus musculus