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Literature summary extracted from

  • Hoffmeister, M.; Piotrowski, M.; Nowitzki, U.; Martin, W.
    Mitochondrial trans-2-enoyl-CoA reductase of wax ester fermentation from Euglena gracilis defines a new family of enzymes involved in lipid synthesis (2005), J. Biol. Chem., 280, 4329-4338.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.44 phage library screening, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional expression as His-tagged enzyme in Escherichia coli Euglena gracilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.44 0.068
-
crotonyl-CoA pH 6.2, 30°C Euglena gracilis
1.3.1.44 0.068
-
crotonyl-CoA recombinant enzyme, pH 6.2, 30°C, with NADH as cofactor Euglena gracilis
1.3.1.44 0.091
-
trans-2-hexenoyl-CoA pH 6.2, 30°C Euglena gracilis
1.3.1.44 0.091
-
trans-2-hexenoyl-CoA recombinant enzyme, pH 6.2, 30°C, with NADH as cofactor Euglena gracilis
1.3.1.44 0.109
-
Biochanin A recombinant enzyme, pH 6.2, 30°C, with crotonyl-CoA as substrate Euglena gracilis
1.3.1.44 0.109
-
Biochanin A cosubstrate crotonyl-CoA, pH 6.2, 30°C Euglena gracilis
1.3.1.44 0.119
-
NADPH recombinant enzyme, pH 6.2, 30°C, with crotonyl-CoA as substrate Euglena gracilis
1.3.1.44 0.119
-
NADPH cosubstrate crotonyl-CoA, pH 6.2, 30°C Euglena gracilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.1.44 mitochondrion
-
Euglena gracilis 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.44 44000
-
gel filtration Euglena gracilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.44 additional information Euglena gracilis enzyme is involved in malonyl-CoA independent lipid synthesis and wax ester fermentation ?
-
?
1.3.1.44 trans-didehydroacyl-CoA + NAD(P)H Euglena gracilis i.e. enoyl-CoA acyl-CoA + NAD(P)+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.44 Euglena gracilis Q5EU90
-
-
1.3.1.44 Euglena gracilis Q5EU90 mitochondrial precursor
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.44
-
Euglena gracilis
1.3.1.44 native enzyme 1687fold to homogeneity in several steps, recombinant His-tagged enzyme from Escherichia coli by affinity chromatography Euglena gracilis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.3.1.44 additional information enzyme is expressed under aerobic and anaerobic conditions Euglena gracilis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.44 0.078
-
mitochondria, anaerobic conditions, substrates crotonyl-CoA and NADH Euglena gracilis
1.3.1.44 0.086
-
mitochondria, aerobic conditions, substrates crotonyl-CoA and NADH Euglena gracilis
1.3.1.44 0.37
-
purified recombinant enzyme, substrates trans-hexenoyl-CoA and NADPH Euglena gracilis
1.3.1.44 0.66
-
purified recombinant enzyme, substrates crotonyl-CoA and NADPH Euglena gracilis
1.3.1.44 1.45
-
purified recombinant enzyme, substrates trans-hexenoyl-CoA and NADH Euglena gracilis
1.3.1.44 1.65
-
purified recombinant enzyme, substrates crotonyl-CoA and NADH Euglena gracilis
1.3.1.44 3.88
-
pH 6.2, 30°C Euglena gracilis
1.3.1.44 3.88
-
purified native enzyme, substrate crotonyl-CoA and NADH Euglena gracilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.44 crotonyl-CoA + NAD(P)H
-
Euglena gracilis butyryl-CoA + NAD(P)+
-
?
1.3.1.44 crotonyl-CoA + NADH + H+
-
Euglena gracilis butyryl-CoA + NAD+
-
r
1.3.1.44 additional information enzyme is involved in malonyl-CoA independent lipid synthesis and wax ester fermentation Euglena gracilis ?
-
?
1.3.1.44 trans-2-hexenoyl-CoA + NAD(P)H
-
Euglena gracilis hexanoyl-CoA + NAD(P)+
-
?
1.3.1.44 trans-didehydroacyl-CoA + NAD(P)H i.e. enoyl-CoA Euglena gracilis acyl-CoA + NAD(P)+
-
?

Subunits

EC Number Subunits Comment Organism
1.3.1.44 monomer 1 * 44000, SDS-PAGE Euglena gracilis
1.3.1.44 monomer 1 x 44000, SDS-PAGE Euglena gracilis

Synonyms

EC Number Synonyms Comment Organism
1.3.1.44 TER
-
Euglena gracilis
1.3.1.44 trans-2-enoyl-CoA reductase
-
Euglena gracilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.44 30
-
assay at Euglena gracilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.44 6.2
-
assay at Euglena gracilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.44 NADH preferred, 2-3fold higher activity compared to NADPH Euglena gracilis
1.3.1.44 NADH about two-to threefold higher specific activites with NADH over NADPH Euglena gracilis
1.3.1.44 NADPH 2-3fold lower activity compared to the preferred NADH Euglena gracilis
1.3.1.44 NADPH about two-to threefold higher specific activites with NADH over NADPH Euglena gracilis