EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.1.44 | phage library screening, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional expression as His-tagged enzyme in Escherichia coli | Euglena gracilis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.3.1.44 | 0.068 | - |
crotonyl-CoA | pH 6.2, 30°C | Euglena gracilis | |
1.3.1.44 | 0.068 | - |
crotonyl-CoA | recombinant enzyme, pH 6.2, 30°C, with NADH as cofactor | Euglena gracilis | |
1.3.1.44 | 0.091 | - |
trans-2-hexenoyl-CoA | pH 6.2, 30°C | Euglena gracilis | |
1.3.1.44 | 0.091 | - |
trans-2-hexenoyl-CoA | recombinant enzyme, pH 6.2, 30°C, with NADH as cofactor | Euglena gracilis | |
1.3.1.44 | 0.109 | - |
Biochanin A | recombinant enzyme, pH 6.2, 30°C, with crotonyl-CoA as substrate | Euglena gracilis | |
1.3.1.44 | 0.109 | - |
Biochanin A | cosubstrate crotonyl-CoA, pH 6.2, 30°C | Euglena gracilis | |
1.3.1.44 | 0.119 | - |
NADPH | recombinant enzyme, pH 6.2, 30°C, with crotonyl-CoA as substrate | Euglena gracilis | |
1.3.1.44 | 0.119 | - |
NADPH | cosubstrate crotonyl-CoA, pH 6.2, 30°C | Euglena gracilis |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.3.1.44 | mitochondrion | - |
Euglena gracilis | 5739 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.3.1.44 | 44000 | - |
gel filtration | Euglena gracilis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.44 | additional information | Euglena gracilis | enzyme is involved in malonyl-CoA independent lipid synthesis and wax ester fermentation | ? | - |
? | |
1.3.1.44 | trans-didehydroacyl-CoA + NAD(P)H | Euglena gracilis | i.e. enoyl-CoA | acyl-CoA + NAD(P)+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.44 | Euglena gracilis | Q5EU90 | - |
- |
1.3.1.44 | Euglena gracilis | Q5EU90 | mitochondrial precursor | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.1.44 | - |
Euglena gracilis |
1.3.1.44 | native enzyme 1687fold to homogeneity in several steps, recombinant His-tagged enzyme from Escherichia coli by affinity chromatography | Euglena gracilis |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.3.1.44 | additional information | enzyme is expressed under aerobic and anaerobic conditions | Euglena gracilis | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.3.1.44 | 0.078 | - |
mitochondria, anaerobic conditions, substrates crotonyl-CoA and NADH | Euglena gracilis |
1.3.1.44 | 0.086 | - |
mitochondria, aerobic conditions, substrates crotonyl-CoA and NADH | Euglena gracilis |
1.3.1.44 | 0.37 | - |
purified recombinant enzyme, substrates trans-hexenoyl-CoA and NADPH | Euglena gracilis |
1.3.1.44 | 0.66 | - |
purified recombinant enzyme, substrates crotonyl-CoA and NADPH | Euglena gracilis |
1.3.1.44 | 1.45 | - |
purified recombinant enzyme, substrates trans-hexenoyl-CoA and NADH | Euglena gracilis |
1.3.1.44 | 1.65 | - |
purified recombinant enzyme, substrates crotonyl-CoA and NADH | Euglena gracilis |
1.3.1.44 | 3.88 | - |
pH 6.2, 30°C | Euglena gracilis |
1.3.1.44 | 3.88 | - |
purified native enzyme, substrate crotonyl-CoA and NADH | Euglena gracilis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.44 | crotonyl-CoA + NAD(P)H | - |
Euglena gracilis | butyryl-CoA + NAD(P)+ | - |
? | |
1.3.1.44 | crotonyl-CoA + NADH + H+ | - |
Euglena gracilis | butyryl-CoA + NAD+ | - |
r | |
1.3.1.44 | additional information | enzyme is involved in malonyl-CoA independent lipid synthesis and wax ester fermentation | Euglena gracilis | ? | - |
? | |
1.3.1.44 | trans-2-hexenoyl-CoA + NAD(P)H | - |
Euglena gracilis | hexanoyl-CoA + NAD(P)+ | - |
? | |
1.3.1.44 | trans-didehydroacyl-CoA + NAD(P)H | i.e. enoyl-CoA | Euglena gracilis | acyl-CoA + NAD(P)+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.3.1.44 | monomer | 1 * 44000, SDS-PAGE | Euglena gracilis |
1.3.1.44 | monomer | 1 x 44000, SDS-PAGE | Euglena gracilis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.1.44 | TER | - |
Euglena gracilis |
1.3.1.44 | trans-2-enoyl-CoA reductase | - |
Euglena gracilis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.3.1.44 | 30 | - |
assay at | Euglena gracilis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.3.1.44 | 6.2 | - |
assay at | Euglena gracilis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.44 | NADH | preferred, 2-3fold higher activity compared to NADPH | Euglena gracilis | |
1.3.1.44 | NADH | about two-to threefold higher specific activites with NADH over NADPH | Euglena gracilis | |
1.3.1.44 | NADPH | 2-3fold lower activity compared to the preferred NADH | Euglena gracilis | |
1.3.1.44 | NADPH | about two-to threefold higher specific activites with NADH over NADPH | Euglena gracilis |