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Literature summary extracted from

  • Ueda, S.; Oda, M.; Imamura, S.; Ohnishi, M.
    Molecular and enzymatic properties of 7alpha-hydroxysteroid dehydrogenase from Pseudomonas sp. B-0831 (2004), J. Biol. Macromol., 4, 33-38.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.159 0.25
-
 cholic acid
-
 Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.159 25000
-
 SDS-PAGE Pseudomonas sp.
1.1.1.159 108000
-
 gel filtration Pseudomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.159 Pseudomonas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.159
-
 Pseudomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.159 chenodeoxycholic acid + NAD+
-
 Pseudomonas sp. 3alpha-hydroxy-7-oxo-5beta-cholan-24-oic acid + NADH
-
 ?
1.1.1.159 cholic acid + NAD+
-
 Pseudomonas sp. 3alpha,12alpha-dihydroxy-7-oxo-5beta-cholan-24-oic acid + NADH
-
 ?
1.1.1.159 additional information catalyzes the oxido-reduction of 7alpha-hydroxy bile acids including glycine and taurine conjugates Pseudomonas sp. ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.159 tetramer 4 * 25000, subunit mass by SDS-PAGE, native mass by gel filtration Pseudomonas sp.

Synonyms

EC Number Synonyms Comment Organism
1.1.1.159 7alpha-HSD
-
 Pseudomonas sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.159 NAD+ highly NAD+ dependent Pseudomonas sp.
1.1.1.159 thio-NAD+ utilized with the same activity as NAD+ Pseudomonas sp.