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Literature summary extracted from
- Purification and characterization of Escherichia coli sulfite reductase and its application in surimi processing (2002), J. Food Sci., 67, 3329-3334.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.8.1.2 | nutrition | quality determination of surimi, purified enzyme increases the reactive SH and gel strength of surimi prepared from frozen mackerel, processing of surimi-based products | Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.2 | Ba2+ | - |
Escherichia coli |  |
| 1.8.1.2 | Ca2+ | strong inhibition | Escherichia coli | |
| 1.8.1.2 | Cd2+ | - |
Escherichia coli | |
| 1.8.1.2 | Co2+ | strong inhibition | Escherichia coli | |
| 1.8.1.2 | Cu2+ | strong inhibition | Escherichia coli | |
| 1.8.1.2 | Fe2+ | strong inhibition | Escherichia coli | |
| 1.8.1.2 | Fe3+ | strong inhibition | Escherichia coli | |
| 1.8.1.2 | Hg2+ | strong inhibition | Escherichia coli | |
| 1.8.1.2 | iodoacetic acid | - |
Escherichia coli | |
| 1.8.1.2 | KCN | complete inhibition at 1 mM | Escherichia coli | |
| 1.8.1.2 | Mn2+ | - |
Escherichia coli | |
| 1.8.1.2 | additional information | inhibitory potency of metal ions, overview, no inhibition by Na+, K+, and Mg2+ | Escherichia coli | |
| 1.8.1.2 | NEM | - |
Escherichia coli | |
| 1.8.1.2 | PCMB | complete inhibition at 1 mM | Escherichia coli | |
| 1.8.1.2 | PMSF | - |
Escherichia coli | |
| 1.8.1.2 | Zn2+ | - |
Escherichia coli | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.2 | 119000 | - |
gel filtration | Escherichia coli |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.1.2 | Escherichia coli | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.1.2 | 579.5fold to homogeneity by ammoniums sulfate fractionation, DEAE ion exchange chromatography, and gel filtration | Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.2 | 10.02 | - |
purified enzyme | Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.1.2 | sulfite + NADPH | - |
Escherichia coli | hydrogen sulfide + NADP+ + H2O | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.1.2 | NADPH-sulfite reductase | - |
Escherichia coli |
| 1.8.1.2 | sulfite reductase | - |
Escherichia coli |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.2 | 25 | - |
- |
Escherichia coli |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.2 | 7.7 | - |
- |
Escherichia coli |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.2 | 6.5 | 8 | 75% activity at pH 6.5 and pH 8.0 | Escherichia coli |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.8.1.2 | 6.5 | 8 | 75% activity at pH 6.5 and pH 8.0 after 30 min at 25°C | Escherichia coli |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.1.2 | FMN | - |
Escherichia coli | |
| 1.8.1.2 | NADPH | - |
Escherichia coli | |
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