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Literature summary extracted from

  • Sgraja, T.; Ulschmid, J.; Becker, K.; Schneuwly, S.; Klebe, G.; Reuter, K.; Heine, A.
    Structural insights into the neuroprotective-acting carbonyl reductase Sniffer of Drosophila melanogaster (2004), J. Mol. Biol., 342, 1613-1624.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.184 sniffer gene, expression as His-tagged enzyme from Escherichia coli strain M15 Drosophila melanogaster

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.1.1.184 purified recombinant enzyme, hanging drop vapour diffusion method, 0.001 ml water with 0.003 ml protein solution and 0.003 ml reservoir solution giving 18% w/v PEG 4000, 0.15 M sodium acetate, 75 mM Tris, pH 8.5, severeal days at 4°C, X-ray diffraction structure determination and analysis at 1.75 A resolution, molecular modeling Drosophila melanogaster

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.184 Drosophila melanogaster
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.184 recombinant His-tagged enzyme by nickel affinity chromatography Drosophila melanogaster

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.184 R-CHOH-R' + NADP+ = R-CO-R' + NADPH + H+ stereospecifc reaction mechanism Drosophila melanogaster
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.184 4-nitrobenzaldehyde + NADPH artificial substrate Drosophila melanogaster 4-nitrobenzyl alcohol + NADP+
-
 ?
1.1.1.184 9,10-phenanthrenequinone + NADPH artificial substrate Drosophila melanogaster ? + NADP+
-
 ?
1.1.1.184 menadione + NADPH artificial substrate Drosophila melanogaster ? + NADP+
-
 ?
1.1.1.184 additional information stereospecific enzyme, substrate binding mechanism, active site cleft Drosophila melanogaster ?
-
 ?
1.1.1.184 pyridin-4-carboxyaldehyde + NADPH artificial substrate Drosophila melanogaster pyridin-4-ylmethanol + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.184 dimer
-
 Drosophila melanogaster

Synonyms

EC Number Synonyms Comment Organism
1.1.1.184 carbonyl reductase
-
 Drosophila melanogaster
1.1.1.184 More enzyme belongs to the short-chain dehydrogenase/reductase family of enzymes Drosophila melanogaster

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.184 NADPH beta-form, cofactor binding site structure Drosophila melanogaster