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Literature summary extracted from

  • Miller, E.S.; Parker, K.N.; Liebl, W.; Lam, D.; Callem, W.; Snead, M.A.; mathur, E.J.; Short, J.M.; Kelly, R.M.
    alpha-D-Galactosidases from Thermotoga species (2001), Methods Enzymol., 330, 246-260.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.22 expression in Escherichia coli Thermotoga neapolitana
3.2.1.22 expression in Escherichia coli Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.22 CuCl2 1 mM, 50% inhibition Thermotoga maritima
3.2.1.22 FeCl2 1 mM, 50% inhibition Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.22 0.075
-
p-nitrophenyl alpha-D-galactopyranoside pH 5, 75°C Thermotoga maritima
3.2.1.22 0.74
-
p-nitrophenyl alpha-D-galactopyranoside pH 7, 98°C Thermotoga neapolitana
3.2.1.22 2.1
-
raffinose pH 5, 75°C Thermotoga maritima
3.2.1.22 5.32
-
raffinose pH 7, 98°C Thermotoga neapolitana
3.2.1.22 15.11
-
melibiose pH 7, 98°C Thermotoga neapolitana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.22 61000
-
1 * 61000, SDS-PAGE Thermotoga neapolitana
3.2.1.22 62000
-
2 * 62000, SDS-PAGE Thermotoga maritima
3.2.1.22 125000
-
gel filtration Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Thermotoga maritima
-
DSMZ3109
-
3.2.1.22 Thermotoga maritima DSM 3109
-
DSMZ3109
-
3.2.1.22 Thermotoga neapolitana
-
DSMZ5068
-
3.2.1.22 Thermotoga neapolitana DSM Z5068
-
DSMZ5068
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.22
-
Thermotoga neapolitana
3.2.1.22
-
Thermotoga maritima

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.22 224
-
activity with p-nitrophenyl alpha-D-galactopyranoside Thermotoga neapolitana
3.2.1.22 225
-
activity with p-nitrophenyl alpha-D-galactopyranoside Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 melibiose + H2O
-
Thermotoga neapolitana D-galactose + alpha-D-glucose
-
?
3.2.1.22 melibiose + H2O
-
Thermotoga maritima D-galactose + alpha-D-glucose
-
?
3.2.1.22 melibiose + H2O
-
Thermotoga maritima DSM 3109 D-galactose + alpha-D-glucose
-
?
3.2.1.22 melibiose + H2O
-
Thermotoga neapolitana DSM Z5068 D-galactose + alpha-D-glucose
-
?
3.2.1.22 additional information slight preference for branched galactosyl residues over straight chain residues Thermotoga neapolitana ?
-
?
3.2.1.22 additional information slight preference for branched galactosyl residues over straight chain residues Thermotoga neapolitana DSM Z5068 ?
-
?
3.2.1.22 o-nitrophenyl alpha-D-galactopyranoside + H2O
-
Thermotoga maritima o-nitrophenol + alpha-D-galactose
-
?
3.2.1.22 o-nitrophenyl alpha-D-galactopyranoside + H2O
-
Thermotoga maritima DSM 3109 o-nitrophenol + alpha-D-galactose
-
?
3.2.1.22 p-nitrophenyl alpha-D-galactopyranoside + H2O
-
Thermotoga neapolitana p-nitrophenol + alpha-D-galactose
-
?
3.2.1.22 p-nitrophenyl alpha-D-galactopyranoside + H2O
-
Thermotoga maritima p-nitrophenol + alpha-D-galactose
-
?
3.2.1.22 p-nitrophenyl alpha-D-galactopyranoside + H2O
-
Thermotoga maritima DSM 3109 p-nitrophenol + alpha-D-galactose
-
?
3.2.1.22 p-nitrophenyl alpha-D-galactopyranoside + H2O
-
Thermotoga neapolitana DSM Z5068 p-nitrophenol + alpha-D-galactose
-
?
3.2.1.22 raffinose + H2O
-
Thermotoga neapolitana sucrose + D-galactose
-
?
3.2.1.22 raffinose + H2O
-
Thermotoga neapolitana DSM Z5068 sucrose + D-galactose
-
?
3.2.1.22 raffinose + H2O
-
Thermotoga maritima sucrose + alpha-D-galactose
-
?
3.2.1.22 raffinose + H2O
-
Thermotoga maritima DSM 3109 sucrose + alpha-D-galactose
-
?
3.2.1.22 stachyose + 2 H2O
-
Thermotoga neapolitana 2 alpha-D-galactose + sucrose
-
?
3.2.1.22 stachyose + 2 H2O 20fold less active than with raffinose Thermotoga maritima 2 alpha-D-galactose + sucrose
-
?
3.2.1.22 stachyose + 2 H2O 20fold less active than with raffinose Thermotoga maritima DSM 3109 2 alpha-D-galactose + sucrose
-
?
3.2.1.22 stachyose + 2 H2O
-
Thermotoga neapolitana DSM Z5068 2 alpha-D-galactose + sucrose
-
?
3.2.1.22 verbascose + H2O
-
Thermotoga neapolitana alpha-D-galactose + alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside
-
?
3.2.1.22 verbascose + H2O 20fold less active than with raffinose Thermotoga maritima alpha-D-galactose + alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.22 dimer 2 * 62000, SDS-PAGE Thermotoga maritima
3.2.1.22 monomer 1 * 61000, SDS-PAGE Thermotoga neapolitana

Synonyms

EC Number Synonyms Comment Organism
3.2.1.22 TmGalA
-
Thermotoga maritima
3.2.1.22 TnGalA
-
Thermotoga neapolitana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.22 90 95
-
Thermotoga maritima
3.2.1.22 99
-
in 50 mM sodium phosphate buffer, pH 7 Thermotoga neapolitana

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.22 75 95 more than 50% of maximal activity at 75°C and at 95°C, at pH 5 Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.22 75
-
pH 7, 20 mM Tris-HCl buffer, 1 mg/ml protein concentration, 50% loss of activity after 7 days Thermotoga maritima
3.2.1.22 80
-
pH 7, 20 mM Tris-HCl buffer, 1 mg/ml protein concentration, 50% loss of activity after 48 h Thermotoga maritima
3.2.1.22 85
-
pH 7, 20 mM Tris-HCl buffer, 1 mg/ml protein concentration, 50% loss of activity after 6 h Thermotoga maritima
3.2.1.22 90
-
pH 7, 20 mM Tris-HCl buffer, 1 mg/ml protein concentration, 50% loss of activity after 70 min Thermotoga maritima
3.2.1.22 100
-
half-life: 2.5 min Thermotoga neapolitana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.22 109
-
raffinose pH 5, 75°C Thermotoga maritima
3.2.1.22 157.3
-
raffinose pH 7, 98°C Thermotoga neapolitana
3.2.1.22 176
-
p-nitrophenyl alpha-D-galactopyranoside pH 5, 75°C Thermotoga maritima
3.2.1.22 234.2
-
melibiose pH 7, 98°C Thermotoga neapolitana
3.2.1.22 302.9
-
p-nitrophenyl alpha-D-galactopyranoside pH 7, 98°C Thermotoga neapolitana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.22 5 5.5
-
Thermotoga maritima
3.2.1.22 7
-
at 90°C in 50 mM sodium phosphate buffer Thermotoga neapolitana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.22 4.5 7 more than 50% of maximal activity at pH 4.5 and pH 7.0, at 75°C Thermotoga maritima

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.22 5 8 75°C, 50 mM citrate-phosphate buffer, less than 40% loss of activity after 5 h Thermotoga maritima

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.22 Thermotoga neapolitana calculation
-
4.9