EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.22 | expression in Escherichia coli | Thermotoga neapolitana |
3.2.1.22 | expression in Escherichia coli | Thermotoga maritima |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.22 | CuCl2 | 1 mM, 50% inhibition | Thermotoga maritima | |
3.2.1.22 | FeCl2 | 1 mM, 50% inhibition | Thermotoga maritima |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.22 | 0.075 | - |
p-nitrophenyl alpha-D-galactopyranoside | pH 5, 75°C | Thermotoga maritima | |
3.2.1.22 | 0.74 | - |
p-nitrophenyl alpha-D-galactopyranoside | pH 7, 98°C | Thermotoga neapolitana | |
3.2.1.22 | 2.1 | - |
raffinose | pH 5, 75°C | Thermotoga maritima | |
3.2.1.22 | 5.32 | - |
raffinose | pH 7, 98°C | Thermotoga neapolitana | |
3.2.1.22 | 15.11 | - |
melibiose | pH 7, 98°C | Thermotoga neapolitana |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 61000 | - |
1 * 61000, SDS-PAGE | Thermotoga neapolitana |
3.2.1.22 | 62000 | - |
2 * 62000, SDS-PAGE | Thermotoga maritima |
3.2.1.22 | 125000 | - |
gel filtration | Thermotoga maritima |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.22 | Thermotoga maritima | - |
DSMZ3109 | - |
3.2.1.22 | Thermotoga maritima DSM 3109 | - |
DSMZ3109 | - |
3.2.1.22 | Thermotoga neapolitana | - |
DSMZ5068 | - |
3.2.1.22 | Thermotoga neapolitana DSM Z5068 | - |
DSMZ5068 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.22 | - |
Thermotoga neapolitana |
3.2.1.22 | - |
Thermotoga maritima |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 224 | - |
activity with p-nitrophenyl alpha-D-galactopyranoside | Thermotoga neapolitana |
3.2.1.22 | 225 | - |
activity with p-nitrophenyl alpha-D-galactopyranoside | Thermotoga maritima |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.22 | melibiose + H2O | - |
Thermotoga neapolitana | D-galactose + alpha-D-glucose | - |
? | |
3.2.1.22 | melibiose + H2O | - |
Thermotoga maritima | D-galactose + alpha-D-glucose | - |
? | |
3.2.1.22 | melibiose + H2O | - |
Thermotoga maritima DSM 3109 | D-galactose + alpha-D-glucose | - |
? | |
3.2.1.22 | melibiose + H2O | - |
Thermotoga neapolitana DSM Z5068 | D-galactose + alpha-D-glucose | - |
? | |
3.2.1.22 | additional information | slight preference for branched galactosyl residues over straight chain residues | Thermotoga neapolitana | ? | - |
? | |
3.2.1.22 | additional information | slight preference for branched galactosyl residues over straight chain residues | Thermotoga neapolitana DSM Z5068 | ? | - |
? | |
3.2.1.22 | o-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Thermotoga maritima | o-nitrophenol + alpha-D-galactose | - |
? | |
3.2.1.22 | o-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Thermotoga maritima DSM 3109 | o-nitrophenol + alpha-D-galactose | - |
? | |
3.2.1.22 | p-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Thermotoga neapolitana | p-nitrophenol + alpha-D-galactose | - |
? | |
3.2.1.22 | p-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Thermotoga maritima | p-nitrophenol + alpha-D-galactose | - |
? | |
3.2.1.22 | p-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Thermotoga maritima DSM 3109 | p-nitrophenol + alpha-D-galactose | - |
? | |
3.2.1.22 | p-nitrophenyl alpha-D-galactopyranoside + H2O | - |
Thermotoga neapolitana DSM Z5068 | p-nitrophenol + alpha-D-galactose | - |
? | |
3.2.1.22 | raffinose + H2O | - |
Thermotoga neapolitana | sucrose + D-galactose | - |
? | |
3.2.1.22 | raffinose + H2O | - |
Thermotoga neapolitana DSM Z5068 | sucrose + D-galactose | - |
? | |
3.2.1.22 | raffinose + H2O | - |
Thermotoga maritima | sucrose + alpha-D-galactose | - |
? | |
3.2.1.22 | raffinose + H2O | - |
Thermotoga maritima DSM 3109 | sucrose + alpha-D-galactose | - |
? | |
3.2.1.22 | stachyose + 2 H2O | - |
Thermotoga neapolitana | 2 alpha-D-galactose + sucrose | - |
? | |
3.2.1.22 | stachyose + 2 H2O | 20fold less active than with raffinose | Thermotoga maritima | 2 alpha-D-galactose + sucrose | - |
? | |
3.2.1.22 | stachyose + 2 H2O | 20fold less active than with raffinose | Thermotoga maritima DSM 3109 | 2 alpha-D-galactose + sucrose | - |
? | |
3.2.1.22 | stachyose + 2 H2O | - |
Thermotoga neapolitana DSM Z5068 | 2 alpha-D-galactose + sucrose | - |
? | |
3.2.1.22 | verbascose + H2O | - |
Thermotoga neapolitana | alpha-D-galactose + alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside | - |
? | |
3.2.1.22 | verbascose + H2O | 20fold less active than with raffinose | Thermotoga maritima | alpha-D-galactose + alpha-D-glucopyranosyl-(1,2)-beta-D-fructofuranoside | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.22 | dimer | 2 * 62000, SDS-PAGE | Thermotoga maritima |
3.2.1.22 | monomer | 1 * 61000, SDS-PAGE | Thermotoga neapolitana |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.22 | TmGalA | - |
Thermotoga maritima |
3.2.1.22 | TnGalA | - |
Thermotoga neapolitana |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 90 | 95 | - |
Thermotoga maritima |
3.2.1.22 | 99 | - |
in 50 mM sodium phosphate buffer, pH 7 | Thermotoga neapolitana |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 75 | 95 | more than 50% of maximal activity at 75°C and at 95°C, at pH 5 | Thermotoga maritima |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 75 | - |
pH 7, 20 mM Tris-HCl buffer, 1 mg/ml protein concentration, 50% loss of activity after 7 days | Thermotoga maritima |
3.2.1.22 | 80 | - |
pH 7, 20 mM Tris-HCl buffer, 1 mg/ml protein concentration, 50% loss of activity after 48 h | Thermotoga maritima |
3.2.1.22 | 85 | - |
pH 7, 20 mM Tris-HCl buffer, 1 mg/ml protein concentration, 50% loss of activity after 6 h | Thermotoga maritima |
3.2.1.22 | 90 | - |
pH 7, 20 mM Tris-HCl buffer, 1 mg/ml protein concentration, 50% loss of activity after 70 min | Thermotoga maritima |
3.2.1.22 | 100 | - |
half-life: 2.5 min | Thermotoga neapolitana |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.22 | 109 | - |
raffinose | pH 5, 75°C | Thermotoga maritima | |
3.2.1.22 | 157.3 | - |
raffinose | pH 7, 98°C | Thermotoga neapolitana | |
3.2.1.22 | 176 | - |
p-nitrophenyl alpha-D-galactopyranoside | pH 5, 75°C | Thermotoga maritima | |
3.2.1.22 | 234.2 | - |
melibiose | pH 7, 98°C | Thermotoga neapolitana | |
3.2.1.22 | 302.9 | - |
p-nitrophenyl alpha-D-galactopyranoside | pH 7, 98°C | Thermotoga neapolitana |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 5 | 5.5 | - |
Thermotoga maritima |
3.2.1.22 | 7 | - |
at 90°C in 50 mM sodium phosphate buffer | Thermotoga neapolitana |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 4.5 | 7 | more than 50% of maximal activity at pH 4.5 and pH 7.0, at 75°C | Thermotoga maritima |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.22 | 5 | 8 | 75°C, 50 mM citrate-phosphate buffer, less than 40% loss of activity after 5 h | Thermotoga maritima |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.2.1.22 | Thermotoga neapolitana | calculation | - |
4.9 |