EC Number | Application | Comment | Organism |
---|---|---|---|
1.3.1.8 | biotechnology | optimization of oil-based extended fermentation of recombinant Streptomyces cinnamonensis, expressing the enzyme from Streptomyces collinus, is used to provide methylmalonyl-CoA precursors for monensin biosynthesis, overview | Streptomyces collinus |
1.3.1.8 | synthesis | oil-based extended fermentation of recombinant Streptomyces cinnamonensis, expressing the enzyme from Streptomyces collinus, is used to provide methylmalonyl-CoA precursors for monensin biosynthesis | Streptomyces collinus |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.3.1.8 | expression in Streptomyces cinnamonensis strains C730.1 and L1 | Streptomyces collinus |
1.3.1.86 | expressed in Streptomyces cinnamonensis strain L1 | Streptomyces collinus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.8 | crotonyl-CoA + reduced acceptor | Streptomyces collinus | enzyme is involved in synthesizing methylmalonyl-CoA precursors for monensin biosynthesis, pathway flux, overview | butyryl-CoA + oxidized acceptor | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.8 | Streptomyces collinus | - |
gene ccr, enzyme CCR | - |
1.3.1.86 | Streptomyces collinus | - |
- |
- |
1.3.1.86 | Streptomyces virginiae | - |
strain C730.1 | - |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.3.1.8 | acyl-CoA + NADP+ = 2,3-dehydroacyl-CoA + NADPH + H+ | enzyme might also belong to EC 1.3.99.3, acyl-CoA dehydrogenase | Streptomyces collinus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.3.1.8 | additional information | fermentation in an oil-based medium | Streptomyces collinus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.3.1.8 | additional information | - |
quantification by determination of monensin A | Streptomyces collinus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.8 | crotonyl-CoA + reduced acceptor | - |
Streptomyces collinus | butyryl-CoA + oxidized acceptor | - |
? | |
1.3.1.8 | crotonyl-CoA + reduced acceptor | enzyme is involved in synthesizing methylmalonyl-CoA precursors for monensin biosynthesis, pathway flux, overview | Streptomyces collinus | butyryl-CoA + oxidized acceptor | - |
? | |
1.3.1.86 | crotonyl-CoA + NADPH + H+ | - |
Streptomyces virginiae | butyryl-CoA + NADP+ | - |
? | |
1.3.1.86 | crotonyl-CoA + NADPH + H+ | - |
Streptomyces collinus | butyryl-CoA + NADP+ | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.1.8 | CCR | ambiguous | Streptomyces collinus |
1.3.1.8 | crotonyl-coenzyme A reductase | - |
Streptomyces collinus |
1.3.1.86 | CCR | - |
Streptomyces virginiae |
1.3.1.86 | CCR | - |
Streptomyces collinus |
1.3.1.86 | crotonyl-coenzyme A reductase | - |
Streptomyces virginiae |
1.3.1.86 | crotonyl-coenzyme A reductase | - |
Streptomyces collinus |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.86 | NADPH | - |
Streptomyces virginiae | |
1.3.1.86 | NADPH | - |
Streptomyces collinus |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.3.1.86 | malfunction | loss of ccr leads to lower levels of the monensin precursor methymalonyl-CoA, relative to coenzyme A | Streptomyces virginiae |
1.3.1.86 | malfunction | loss of ccr leads to lower levels of the monensin precursor methymalonyl-CoA, relative to coenzyme A | Streptomyces collinus |
1.3.1.86 | physiological function | CCR plays a significant role in providing methylmalonyl-CoA for monensin biosynthesis in oil-based 10-day fermentations of Streptomyces cinnamonensis | Streptomyces virginiae |
1.3.1.86 | physiological function | CCR plays a significant role in providing methylmalonyl-CoA for monensin biosynthesis in oil-based 10-day fermentations of Streptomyces cinnamonensis | Streptomyces collinus |