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Literature summary extracted from

  • Jagadeesh, B.H.; Prabha, T.N.
    beta-Hexosaminidase, an enzyme from ripening bell capsicum (Capsicum annuum var variata) (2002), Phytochemistry, 61, 295-300.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.52 EDTA 1 mM, activation of isoenzyme I to 115.2% of control Capsicum annuum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 Ca2+ 1 mM, 16% inhibition of isoenzyme II, slight activation of isoenzyme I Capsicum annuum
3.2.1.52 Cu2+ 1 mM, 91% inhibition of isoenzyme I, 22% inhibition of isoenzyme II Capsicum annuum
3.2.1.52 Hg2+ 1 mM, 97% inhibition of isoenzyme I, 95% inhibition of isoenzyme II Capsicum annuum
3.2.1.52 Mg2+ 1 mM, 33% inhibition of isoenzyme II, slight activation of isoenzyme I Capsicum annuum
3.2.1.52 Zn2+ 1 mM, 86% inhibition of isoenzyme I, 62% inhibition of isoenzyme II Capsicum annuum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 1.75
-
 p-nitrophenyl-N-acetyl-beta-D-glucosaminide isoenzyme II Capsicum annuum
3.2.1.52 3
-
 p-nitrophenyl-N-acetyl-beta-D-glucosaminide isoenzyme I Capsicum annuum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.52 Ca2+ 1 mM, activation of isoenzyme I to 110.8% of control Capsicum annuum
3.2.1.52 Mg2+ 1 mM, activation of isoenzyme I to 109.5% of control Capsicum annuum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 85000
-
 1 * 85000, isoenzyme II, SDS-PAGE Capsicum annuum

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Capsicum annuum
-
 var. variata
-
3.2.1.52 Carica papaya
-
-
-
3.2.1.52 Mangifera indica
-
-
-
3.2.1.52 Musca sp.
-
 banana
-
3.2.1.52 Solanum lycopersicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 isoform I and II Capsicum annuum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.52 fruit
-
 Solanum lycopersicum
-
3.2.1.52 fruit
-
 Capsicum annuum
-
3.2.1.52 fruit
-
 Carica papaya
-
3.2.1.52 fruit
-
 Mangifera indica
-
3.2.1.52 fruit
-
 Musca sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 9.31
-
 isoenzyme II Capsicum annuum
3.2.1.52 10.11
-
 isoenzyme I Capsicum annuum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 p-nitrophenyl-N-acetyl-beta-D-glucosaminide + H2O
-
 Capsicum annuum p-nitrophenol + beta-D-N-acetyl-glucosamine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.52 monomer 1 * 85000, isoenzyme II, SDS-PAGE Capsicum annuum

Synonyms

EC Number Synonyms Comment Organism
3.2.1.52 beta-hexosaminidase I
-
 Capsicum annuum
3.2.1.52 beta-hexosaminidase II
-
 Capsicum annuum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.52 37 47 isoenzyme I Capsicum annuum
3.2.1.52 47
-
 isoenzyme II Capsicum annuum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.52 57
-
 15 min, isoenzyme II is stable, isoenzyme I loses 30% of the initial activity Capsicum annuum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 4.6
-
 isoenzyme I Capsicum annuum
3.2.1.52 5
-
 isoenzyme II Capsicum annuum