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Literature summary extracted from

  • Clemente, M.T.; Marquez, A.J.
    Site-directed mutagenesis of Glu-297 from the alpha-polypeptide of Phaseolus vulgaris glutamine synthetase alters kinetic and structural properties and confers resistance to L-methionine sulfoximine (1999), Plant Mol. Biol., 40, 835-845.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.1.2 expressed in Escherichia coli ET8894 Phaseolus vulgaris

Protein Variants

EC Number Protein Variants Comment Organism
6.3.1.2 E297A Km for ammonium strongly increased, minor effects on activity in forward reaction, reverse reaction activity strongly decreased, strong effect on binding behavior to a 2',5'-ADP-sepharose Phaseolus vulgaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.1.2 L-methionine sulfoximine forward reaction: complete inhibition of wild type enzyme, only slight inhibition of mutant enzyme, reverse reaction: slight inhibition of mutant and wild-type enzyme Phaseolus vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.1.2 0.3
-
 ATP wild-type and mutant enzyme, pH 7.5, 37°C Phaseolus vulgaris
6.3.1.2 2
-
 L-glutamate mutant enzyme, pH 7.5, 37°C Phaseolus vulgaris
6.3.1.2 7
-
 L-glutamate wild-type enzyme, pH 7.5, 37°C Phaseolus vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.1.2 43000
-
 SDS-PAGE Phaseolus vulgaris
6.3.1.2 350000
-
 gel filtration Phaseolus vulgaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.2 ATP + glutamate + NH4+ Phaseolus vulgaris central enzyme of nitrogen metabolism, ammonia assimilation ADP + phosphate + L-glutamine
-
 r

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.2 Phaseolus vulgaris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.2 recombinant mutant and wild-type enzyme Phaseolus vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.2 ATP + glutamate + NH4+
-
 Phaseolus vulgaris ADP + phosphate + L-glutamine
-
 r
6.3.1.2 ATP + glutamate + NH4+ central enzyme of nitrogen metabolism, ammonia assimilation Phaseolus vulgaris ADP + phosphate + L-glutamine
-
 r

Subunits

EC Number Subunits Comment Organism
6.3.1.2 octamer 8 * 43000, subunits by SDS-PAGE, native mass by gel filtration Phaseolus vulgaris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.1.2 37
-
 forward reaction of wild-type and mutant enzyme, reverse reaction of wild-type enzyme Phaseolus vulgaris
6.3.1.2 45
-
 reverse reaction of mutant enzyme Phaseolus vulgaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.1.2 7.5
-
 wild-type and mutant enzyme, forward and reverse reaction Phaseolus vulgaris