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Literature summary extracted from

  • Shanklin, J.
    Overexpression and purification of the Escherichia coli inner membrane enzyme acyl-acyl carrier protein synthase in an active form (2000), Protein Expr. Purif., 18, 355-360.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.20 expressed in Escherichia coli BL21(DE3) and C41(DE3) Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.2.1.20 membrane inner membrane Escherichia coli 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.20 Mg2+ required Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.20 80600
-
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.20 ATP + an acid + [acyl-carrier protein] Escherichia coli synthesis of activated acyl-carrier protein that is essential as a carrier of acyl-intermediates, donor for activated acyl groups for the synthesis of lipids and lipid derivatives, functions as 2-acylglycerophosphoethanolamine acyltransferase AMP + diphosphate + acyl-[acyl-carrier protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.20 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.20 recombinant proteins using His-tag Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.2.1.20 0.022
-
 transfected Escherichia coli BL21(DE3) with lactose as inducer and palmitic acid as substrate Escherichia coli
6.2.1.20 0.071
-
 transfected Escherichia coli C41(DE3) with IPTG as inducer and palmitic acid as substrate Escherichia coli
6.2.1.20 1.32
-
 purified enzyme from Escherichia coli C41(DE3) with palmitic acid as substrate Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.20 ATP + an acid + [acyl-carrier protein] synthesis of activated acyl-carrier protein that is essential as a carrier of acyl-intermediates, donor for activated acyl groups for the synthesis of lipids and lipid derivatives, functions as 2-acylglycerophosphoethanolamine acyltransferase Escherichia coli AMP + diphosphate + acyl-[acyl-carrier protein]
-
 ?
6.2.1.20 ATP + an acid + [acyl-carrier protein] fatty acids with chain lengths between 8 and 18 carbons serve as substrates Escherichia coli AMP + diphosphate + acyl-[acyl-carrier protein]
-
 ?