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Literature summary extracted from
- beta-Galactosidase of Penicillium chrysogenum: production, purification, and characterization of the enzyme (2001), Protein Expr. Purif., 21, 24-29.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.23 | 1.81 | - |
o-nitrophenyl beta-D-galactopyranoside | - |
Penicillium chrysogenum |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.23 | intracellular | - |
Penicillium chrysogenum | 5622 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.23 | 66000 | - |
4 * 66000, SDS-PAGE | Penicillium chrysogenum |
| 3.2.1.23 | 270000 | - |
gel filtration | Penicillium chrysogenum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.23 | Penicillium chrysogenum | - |
NCAIM 00237 | - |
| 3.2.1.23 | Penicillium chrysogenum NCAIM 00237 | - |
NCAIM 00237 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.23 | - |
Penicillium chrysogenum |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.23 | mycelium | - |
Penicillium chrysogenum | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.23 | 5.84 | - |
- |
Penicillium chrysogenum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.23 | o-nitrophenyl beta-D-galactopyranoside + H2O | - |
Penicillium chrysogenum | o-nitrophenol + beta-D-galactose | - |
? | |
| 3.2.1.23 | o-nitrophenyl beta-D-galactopyranoside + H2O | - |
Penicillium chrysogenum NCAIM 00237 | o-nitrophenol + beta-D-galactose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.23 | tetramer | 4 * 66000, SDS-PAGE | Penicillium chrysogenum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.23 | 30 | - |
- |
Penicillium chrysogenum |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.23 | 20 | 40 | 20°C: about 65% of maximal activity, 35°C: about 85% of maximal activity, 40°C: about 25% of maximal activity | Penicillium chrysogenum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.23 | 4 | 5 | - |
Penicillium chrysogenum |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.23 | 3.5 | 6.5 | pH 3.0: about 15% of maximal activity, pH 3.5: about 85% of maximal activity, pH 6.5: about 50% of maximal activity, pH 7.0: about 35% of maximal activity | Penicillium chrysogenum |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.2.1.23 | Penicillium chrysogenum | - |
- |
4.9 |
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