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Literature summary extracted from

  • De-Eknamkul, W.; Ellis, B.E.
    Purification and characterization of prephenate aminotransferase from Anchusa officinalis cell cultures (1988), Arch. Biochem. Biophys., 267, 87-94.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.78 3,4-dihydroxyphenyllactic acid metabolite of rosmarinic acid Anchusa officinalis
2.6.1.78 additional information enzyme shows substrate inhibition, but no feedback inhibition by L-phenylalanine and L-tyrosine Anchusa officinalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.78 0.08
-
L-aspartate pH 9.0, 40°C Anchusa officinalis
2.6.1.78 0.08
-
prephenate pH 9.0, 40°C Anchusa officinalis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.78 44000
-
2 * 44000 + 2 * 57000, alpha2beta2-structure, SDS-PAGE Anchusa officinalis
2.6.1.78 57000
-
2 * 44000 + 2 * 57000, alpha2beta2-structure, SDS-PAGE Anchusa officinalis
2.6.1.78 220000
-
gel filtration Anchusa officinalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.78 prephenate + L-aspartate Anchusa officinalis transamination L-arogenate + oxaloacetate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.78 Anchusa officinalis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.78 native enzyme 41fold to homogeneity by anion exchange chromatography, chromatofocusing using the major active fraction with an isoelectric point of 5.4, and gel filtration Anchusa officinalis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.78 cell culture rosmarinic acid-producing cells Anchusa officinalis
-
2.6.1.78 additional information activity during cell cycle Anchusa officinalis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.78 0.34
-
purified enzyme Anchusa officinalis

Storage Stability

EC Number Storage Stability Organism
2.6.1.78 -70°C, purified enzyme, 50 mM Tris-HCl, pH 8.2, 5 mM 2-mercaptoethanol, 150 mM sucrose, 1 mM EDTA, stable for at least 5 months Anchusa officinalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.78 2-oxoglutarate + L-aspartate
-
Anchusa officinalis L-glutamate + oxaloacetate
-
?
2.6.1.78 4-hydroxyphenylpyruvate + L-aspartate
-
Anchusa officinalis L-tyrosine + oxaloacetate
-
?
2.6.1.78 glyoxylate + L-aspartate
-
Anchusa officinalis glycine + oxaloacetate
-
?
2.6.1.78 indolepyruvate + L-aspartate
-
Anchusa officinalis L-tryptophan + oxaloacetate
-
?
2.6.1.78 additional information substrate specificity, highest activity with 2-oxoglutarate and L-aspartate as substrates, low activity with indolepyruvate and glyoxylate Anchusa officinalis ?
-
?
2.6.1.78 phenylpyruvate + L-aspartate
-
Anchusa officinalis L-phenylalanine + oxaloacetate
-
?
2.6.1.78 prephenate + L-aspartate transamination Anchusa officinalis L-arogenate + oxaloacetate
-
?
2.6.1.78 prephenate + L-glutamate transamination, L-glutamate is about 3fold less effective than L-aspartate Anchusa officinalis L-arogenate + 2-oxoglutarate
-
?
2.6.1.78 pyruvate + L-aspartate
-
Anchusa officinalis L-alanine + oxaloacetate
-
?

Subunits

EC Number Subunits Comment Organism
2.6.1.78 tetramer 2 * 44000 + 2 * 57000, alpha2beta2-structure, SDS-PAGE Anchusa officinalis

Synonyms

EC Number Synonyms Comment Organism
2.6.1.78 Pat
-
Anchusa officinalis
2.6.1.78 prephenate aminotransferase
-
Anchusa officinalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.78 60 70
-
Anchusa officinalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.78 9
-
-
Anchusa officinalis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.6.1.78 Anchusa officinalis minor fraction, chromatofocussing
-
5
2.6.1.78 Anchusa officinalis major fraction, chromatofocussing
-
5.4