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Literature summary extracted from

  • Wan, J.T.; Jarrett, J.T.
    Electron acceptor specificity of ferredoxin (flavodoxin):NADP+ oxidoreductase from Escherichia coli (2002), Arch. Biochem. Biophys., 406, 116-126.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.18.1.2 Ferredoxin inhibition of electron transfer at higher electron acceptor rate Escherichia coli
1.18.1.2 flavodoxinI/II inhibition of electron transfer at higher electron acceptor rate Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18.1.2 additional information
-
 additional information thermodynamics and kinetics, measurement of direct electron transfer by stopped-flow spectrophotometry Escherichia coli
1.18.1.2 0.012
-
 Ferredoxin pH 8.0, 25°C Escherichia coli
1.18.1.2 0.012
-
 reduced ferredoxin pH 8.0, 25°C Escherichia coli
1.19.1.1 0.004
-
 reduced flavodoxin II pH 8.0, 25°C Escherichia coli
1.19.1.1 0.0076
-
 reduced flavodoxin I pH 8.0, 25°C Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.18.1.2 FMNH2 + NADP+ Escherichia coli
-
 FMN + NADPH + H+
-
 ?
1.18.1.2 additional information Escherichia coli the enzyme reduces flavodoxin I and flavodoxin II, reaction of EC 1.19.1.1, and ferredoxin, ferredoxin being the kinetically and thermodynamically preferred partner. Ferredoxin binds to FNR with high affinity (Kd below 0.5 microM) and is reduced under single-turnover conditions. Flavodoxin I and flavodoxin II show affinities about 4- to 7fold weaker and reduction rates that are 10- to 100fold slower than those for ferredoxin ?
-
 ?
1.18.1.2 oxidized ferredoxin + NADPH Escherichia coli
-
 reduced ferredoxin + NADP+
-
 r
1.18.1.2 reduced ferredoxin + NADP+ Escherichia coli
-
 oxidized ferredoxin + NADPH + H+
-
 ?
1.19.1.1 FMNH2 + NADP+ Escherichia coli
-
 FMN + NADPH + H+
-
 ?
1.19.1.1 additional information Escherichia coli the enzyme reduces flavodoxin I, flavodoxin II and ferredoxin, ferredoxin being the kinetically and thermodynamically preferred partner, i.e. reaction of EC 1.18.2.1. Flavodoxin I and flavodoxin II behave similarly with respect to FNR, with affinities about 4- to 7fold weaker and reduction rates that are 10- to 100fold slower than those for ferredoxin. Flavodoxin I and flavodoxin II can obtain electrons from reduced Fd at rates that are comparable to those obtained with reduced FNR ?
-
 ?
1.19.1.1 reduced flavodoxin I + NADP+ Escherichia coli
-
 oxidized flavodoxin I + NADPH + H+
-
 ?
1.19.1.1 reduced flavodoxin II + NADP+ Escherichia coli
-
 oxidized flavodoxin II + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.18.1.2 Escherichia coli
-
-
-
1.18.1.2 Escherichia coli P28861 enzyme catalyzes reactions of both EC 1.18.1.2 and EC 1.19.1.1
-
1.19.1.1 Escherichia coli P28861 enzyme catalyzes reactions of both EC 1.18.1.2 and EC 1.19.1.1
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.18.1.2 2 reduced ferredoxin + NADP+ + H+ = 2 oxidized ferredoxin + NADPH reaction mechanism Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.1.2 FMNH2 + NADP+
-
 Escherichia coli FMN + NADPH + H+
-
 ?
1.18.1.2 additional information specificity for tightly bound electron acceptors, overview Escherichia coli ?
-
 ?
1.18.1.2 additional information the enzyme reduces flavodoxin I and flavodoxin II, reaction of EC 1.19.1.1, and ferredoxin, ferredoxin being the kinetically and thermodynamically preferred partner. Ferredoxin binds to FNR with high affinity (Kd below 0.5 microM) and is reduced under single-turnover conditions. Flavodoxin I and flavodoxin II show affinities about 4- to 7fold weaker and reduction rates that are 10- to 100fold slower than those for ferredoxin Escherichia coli ?
-
 ?
1.18.1.2 oxidized ferredoxin + NADPH
-
 Escherichia coli reduced ferredoxin + NADP+
-
 r
1.18.1.2 oxidized ferredoxin + NADPH ferredoxin is the preferred electron acceptor Escherichia coli reduced ferredoxin + NADP+
-
 r
1.18.1.2 reduced ferredoxin + NADP+
-
 Escherichia coli oxidized ferredoxin + NADPH + H+
-
 r
1.18.1.2 reduced ferredoxin + NADP+
-
 Escherichia coli oxidized ferredoxin + NADPH + H+
-
 ?
1.18.1.2 reduced flavodoxin I + NADP+
-
 Escherichia coli oxidized flavodoxin I + NADPH + H+
-
 r
1.18.1.2 reduced flavodoxin II + NADP+
-
 Escherichia coli oxidized flavodoxin II + NADPH + H+
-
 r
1.19.1.1 FMNH2 + NADP+
-
 Escherichia coli FMN + NADPH + H+
-
 ?
1.19.1.1 additional information the enzyme reduces flavodoxin I, flavodoxin II and ferredoxin, ferredoxin being the kinetically and thermodynamically preferred partner, i.e. reaction of EC 1.18.2.1. Flavodoxin I and flavodoxin II behave similarly with respect to FNR, with affinities about 4- to 7fold weaker and reduction rates that are 10- to 100fold slower than those for ferredoxin. Flavodoxin I and flavodoxin II can obtain electrons from reduced Fd at rates that are comparable to those obtained with reduced FNR Escherichia coli ?
-
 ?
1.19.1.1 reduced flavodoxin I + NADP+
-
 Escherichia coli oxidized flavodoxin I + NADPH + H+
-
 ?
1.19.1.1 reduced flavodoxin II + NADP+
-
 Escherichia coli oxidized flavodoxin II + NADPH + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.18.1.2 ferredoxin (flavodoxin):NADP+ oxidoreductase
-
 Escherichia coli
1.18.1.2 FNR
-
 Escherichia coli
1.19.1.1 ferredoxin (flavodoxin):NADP+ oxidoreductase
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.18.1.2 25
-
 assay at Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.18.1.2 0.0039
-
 reduced flavodoxin II pH 8.0, 25°C Escherichia coli
1.18.1.2 0.0042
-
 reduced flavodoxin I pH 8.0, 25°C Escherichia coli
1.18.1.2 0.15
-
 reduced ferredoxin pH 8.0, 25°C Escherichia coli
1.19.1.1 0.0039
-
 reduced flavodoxin II pH 8.0, 25°C Escherichia coli
1.19.1.1 0.0042
-
 reduced flavodoxin I pH 8.0, 25°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.18.1.2 8
-
 assay at, steady-state kinetics Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.18.1.2 FAD involved in electron transfer in the reaction Escherichia coli
1.18.1.2 NADP+
-
 Escherichia coli
1.18.1.2 NADPH
-
 Escherichia coli
1.19.1.1 NADP+
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.18.1.2 0.05 0.1 Ferredoxin pH 8.0, 25°C Escherichia coli
1.18.1.2 0.05 0.1 flavodoxin I pH 8.0, 25°C Escherichia coli
1.18.1.2 0.05 0.1 flavodoxin II pH 8.0, 25°C Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.18.1.2 12.5
-
 reduced ferredoxin pH 8.0, 25°C Escherichia coli
1.18.1.2 19
-
 FMNH2 pH 8.0, 25°C Escherichia coli
1.19.1.1 550
-
 reduced flavodoxin I pH 8.0, 25°C Escherichia coli
1.19.1.1 980
-
 reduced flavodoxin II pH 8.0, 25°C Escherichia coli
1.19.1.1 19000
-
 FMNH2 pH 8.0, 25°C Escherichia coli