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Literature summary extracted from

  • Seo, D.; Kamino, K.; Inoue, K.; Sakurai, H.
    Purification and characterization of ferredoxin-NADP+ reductase encoded by Bacillus subtilis yumC (2004), Arch. Microbiol., 182, 80-89.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18.1.2 additional information
-
 additional information below 0.1 mM, oxidized ferredoxin with NADH, pH 7.0, 24°C Bacillus subtilis
1.18.1.2 0.54
-
 oxidized ferredoxin with NADPH, pH 7.0, 24°C Bacillus subtilis
1.18.1.2 0.57
-
 NADPH 2,6-dichlorophenolindophenol reduction, pH 7.0, 24°C Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.18.1.2 40000
-
 2 * 40000, SDS-PAGE Bacillus subtilis
1.18.1.2 94000
-
 gel filtration Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.18.1.2 reduced ferredoxin + NADP+ Bacillus subtilis
-
 oxidized ferredoxin + NADPH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.18.1.2 Bacillus subtilis
-
 gene yumC
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.18.1.2 native enzyme from cell extract 86fold to homogeneity by several chromatographic steps Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.18.1.2 33.8
-
 purified enzyme Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.1.2 additional information the enzyme also shows NAD(P)H oxidase activity, overview Bacillus subtilis ?
-
 ?
1.18.1.2 NADPH + H+ + oxidized 2,6-dichlorophenolindophenol diaphorase activity, no activity with NADH Bacillus subtilis NADP+ + reduced 2,6-dichlorophenolindophenol
-
 ?
1.18.1.2 oxidized ferredoxin + NADH + H+
-
 Bacillus subtilis reduced ferredoxin + NAD+
-
 r
1.18.1.2 reduced ferredoxin + NADP+
-
 Bacillus subtilis oxidized ferredoxin + NADPH
-
 ?
1.18.1.2 reduced ferredoxin + NADP+
-
 Bacillus subtilis oxidized ferredoxin + NADPH
-
 r

Subunits

EC Number Subunits Comment Organism
1.18.1.2 dimer 2 * 40000, SDS-PAGE Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
1.18.1.2 FNR
-
 Bacillus subtilis
1.18.1.2 YumC
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.18.1.2 24
-
 assay at Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.18.1.2 0.4
-
 NADH ferredoxin reduction, pH 7.0, 24°C Bacillus subtilis
1.18.1.2 22.7
-
 NADPH 2,6-dichlorophenolindophenol reduction, pH 7.0, 24°C Bacillus subtilis
1.18.1.2 35.4
-
 NADPH ferredoxin reduction, pH 7.0, 24°C Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.18.1.2 7
-
 assay at Bacillus subtilis

Cofactor

EC Number Cofactor Comment Organism Structure
1.18.1.2 FAD
-
 Bacillus subtilis
1.18.1.2 NADP+
-
 Bacillus subtilis
1.18.1.2 NADPH reverse reaction: preferred cofactor, poor activity with NADH Bacillus subtilis