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Literature summary extracted from

  • Libisch, B.; Michaelson, L.V.; Lewis, M.J.; Shewry, P.R.; Napier, J.A.
    Chimeras of DELTA6-fatty acid and DELTA8-sphingolipid desaturases (2000), Biochem. Biophys. Res. Commun., 279, 779-785.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.4
-
 Borago officinalis
1.14.19.29 expressed as chimera protein with DELTA6 fatty acid desaturase in Saccharomyces cervisiae Borago officinalis

Protein Variants

EC Number Protein Variants Comment Organism
1.14.19.4 additional information chimeras of DELTA6-fatty acid and DELTA8-sphingolipid desaturases Borago officinalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.19.29 (4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+ Borago officinalis 39.8% conversion rate (4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
 ?
1.14.19.29 (4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+ Borago officinalis 7.5% conversion rate (4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.4 Borago officinalis Q9FR82
-
-
1.14.19.29 Borago officinalis Q9FR82
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.4 phytosphinganine + reduced acceptor + O2
-
 Borago officinalis trans-8-sphingenine + cis-8-sphingenine + acceptor + H2O
-
 ?
1.14.19.29 (4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+ 39.8% conversion rate Borago officinalis (4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
 ?
1.14.19.29 (4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+ 7.5% conversion rate Borago officinalis (4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
 ?
1.14.19.29 additional information no activity with palmitoleic acid, linoleic acid, and alpha-linolenic acid Borago officinalis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.19.4 Boofd8
-
 Borago officinalis
1.14.19.4 DELTA8-sphingolipid desaturase
-
 Borago officinalis
1.14.19.29 DELTA8-sphingolipid desaturase
-
 Borago officinalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.29 cytochrome b5
-
 Borago officinalis