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Literature summary extracted from

  • Warrilow, A.G.; Lamb, D.C.; Kelly, D.E.; Kelly, S.L.
    Phanerochaete chrysosporium NADPH-cytochrome P450 reductase kinetic mechanism (2002), Biochem. Biophys. Res. Commun., 299, 189-195.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.2.4 native and truncated form expressed in Escherichia coli BL21(DE3)pLysS as His tag fusion protein Phanerodontia chrysosporium

Protein Variants

EC Number Protein Variants Comment Organism
1.6.2.4 DELTA1-23 truncated enzyme Phanerodontia chrysosporium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.4 0.0019
-
 NADPH full length enzyme, pH 7.8, 25°C Phanerodontia chrysosporium
1.6.2.4 0.0022
-
 NADPH truncated enzyme, pH 7.8, 25°C Phanerodontia chrysosporium
1.6.2.4 0.011
-
 ferricytochrome c full length enzyme, pH 7.8, 25°C Phanerodontia chrysosporium
1.6.2.4 0.012
-
 ferricytochrome c truncated enzyme, pH 7.8, 25°C Phanerodontia chrysosporium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.2.4 membrane
-
 Phanerodontia chrysosporium 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.2.4 79300
-
 theoretical mass, truncated enzyme Phanerodontia chrysosporium
1.6.2.4 81600
-
 theoretical mass, full length enzyme Phanerodontia chrysosporium
1.6.2.4 85900
-
 SDS-PAGE, truncated enzyme Phanerodontia chrysosporium
1.6.2.4 88200
-
 SDS-PAGE, full length enzyme Phanerodontia chrysosporium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.2.4 2 ferricytochrome P450 + NADPH Phanerodontia chrysosporium transfer of electrons during the CYP catalytic cycle 2 ferrocytochrome P450 + NADP+ + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.4 Phanerodontia chrysosporium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.4 recombinant enzymes using His-tag Phanerodontia chrysosporium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.2.4 3.27
-
 purified full length enzyme, pH 7.8, 25°C Phanerodontia chrysosporium
1.6.2.4 14.76
-
 purified truncated enzyme, pH 7.8, 25°C Phanerodontia chrysosporium

Storage Stability

EC Number Storage Stability Organism
1.6.2.4 -80°C, 20 mM phosphate buffer, pH 7.5, 20% glycerol Phanerodontia chrysosporium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.4 2 ferricytochrome c + NADPH
-
 Phanerodontia chrysosporium 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.6.2.4 2 ferricytochrome P450 + NADPH transfer of electrons during the CYP catalytic cycle Phanerodontia chrysosporium 2 ferrocytochrome P450 + NADP+ + H+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.6.2.4 NADPH-cytochrome P450 reductase
-
 Phanerodontia chrysosporium
1.6.2.4 NADPH-ferrihemoprotein reductase
-
 Phanerodontia chrysosporium
1.6.2.4 NADPH:ferrihemoprotein oxidoreductase
-
 Phanerodontia chrysosporium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.2.4 19
-
 ferricytochrome c full length enzyme, pH 7.8, 25°C Phanerodontia chrysosporium
1.6.2.4 42
-
 ferricytochrome c truncated enzyme, pH 7.8, 25°C Phanerodontia chrysosporium

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.4 FAD together with FMN Phanerodontia chrysosporium
1.6.2.4 FMN together with FAD Phanerodontia chrysosporium
1.6.2.4 NADPH
-
 Phanerodontia chrysosporium