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Literature summary extracted from

  • Jala, V.R.; Appaji Rao, N.; Savithri, H.S.
    Identification of amino acid residues, essential for maintaining the tetrameric structure of sheep liver cytosolic serine hydroxymethyltransferase, by targeted mutagenesis (2003), Biochem. J., 369, 469-476.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.1.2.1 C203S no loss of activity Ovis aries
2.1.2.1 D227N nearly complete loss of activity, enzyme exists as dimer Ovis aries
2.1.2.1 H304A nearly complete loss of activity, enzyme exists as dimer Ovis aries
2.1.2.1 H306A partial loss of activity, 60% of the enzyme exists as dimer Ovis aries
2.1.2.1 H356A partial loss of activity, 80% of the enzyme exists as dimer Ovis aries
2.1.2.1 K71Q nearly complete loss of activity, enzyme exists as dimer Ovis aries
2.1.2.1 R80A nearly complete loss of activity, enzyme exists as dimer Ovis aries
2.1.2.1 R98A mutant enzyme is present in the insoluble fraction Ovis aries
2.1.2.1 S202C partial loss of activity, 55% of the enzyme exists as dimer Ovis aries
2.1.2.1 W110A mutant enzyme is present in the insoluble fraction Ovis aries
2.1.2.1 W110F no loss of activity Ovis aries
2.1.2.1 Y72F partial loss of activity, 30% of the enzyme exists as dimer Ovis aries
2.1.2.1 Y81F 20% of the enzyme exists as dimer Ovis aries

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.2.1 cytosol
-
 Ovis aries 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.1 53000
-
 4 * 53000, wild-type enzyme and mutant enzymes C203F and W110F, SDS-PAGE Ovis aries
2.1.2.1 210000
-
 gel filtration Ovis aries

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Ovis aries
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.2.1 liver
-
 Ovis aries
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 L-Ser + tetrahydrofolate
-
 Ovis aries Gly + 5,10-methylenetetrahydrofolate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.2.1 tetramer 4 * 53000, wild-type enzyme and mutant enzymes C203F and W110F, SDS-PAGE Ovis aries

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.1 pyridoxal 5'-phosphate dimeric form of some mutant enzymes contains no pyridoxal 5'-phosphate Ovis aries