EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.31 | overexpression of His-tagged wild-type and mutant enzymes in strain BL21(DE3) | Escherichia coli |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.5.1.31 | co-crystal structure of UPPS in complex with (2E,6E)-farnesyl diphosphate | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.5.1.31 | F89A | site-directed mutagenesis, mutant shows highly increased Km for isopentenyl diphosphate with farnesyl diphosphate and slightly with geranylgeranyl diphosphate compared to the wild-type enzyme | Escherichia coli |
2.5.1.31 | F89A | mutation increases (2E,6E)-farnesyl diphosphate and geranylgeranyl diphosphate Km values, indicating that these amino acids are important for substrate binding, but do not determine substrate specificity | Escherichia coli |
2.5.1.31 | L85A | site-directed mutagenesis, mutant shows highly increased Km for isopentenyl diphosphate with farnesyl diphosphate and slightly with geranylgeranyl diphosphate compared to the wild-type enzyme | Escherichia coli |
2.5.1.31 | L85A | mutation increases (2E,6E)-farnesyl diphosphate and geranylgeranyl diphosphate Km values, indicating that these amino acids are important for substrate binding, but do not determine substrate specificity | Escherichia coli |
2.5.1.31 | L88A | site-directed mutagenesis, mutant shows highly increased Km for isopentenyl diphosphate with farnesyl diphosphate and slightly with geranylgeranyl diphosphate compared to the wild-type enzyme | Escherichia coli |
2.5.1.31 | L88A | mutation increases (2E,6E)-farnesyl diphosphate and geranylgeranyl diphosphate Km values, indicating that these amino acids are important for substrate binding, but do not determine substrate specificity | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.31 | additional information | farnesol (1 mM) lacking the diphosphate does not inhibit the UPPS reaction; no inhibition by farnesol | Escherichia coli |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.31 | additional information | - |
additional information | steady-state kinetics, single turnover measurements with substrate geranylgeranyl diphosphate | Escherichia coli | |
2.5.1.31 | 0.0003 | - |
geranylgeranyl diphosphate | pH 7.5, 25°C, wild-type enzyme | Escherichia coli | |
2.5.1.31 | 0.0003 | - |
geranylgeranyl diphosphate | wild type, 25°C, pH 7.5 | Escherichia coli | |
2.5.1.31 | 0.0004 | - |
farnesyl diphosphate | pH 7.5, 25°C, wild-type enzyme | Escherichia coli | |
2.5.1.31 | 0.0004 | - |
(2E,6E)-farnesyl diphosphate | wild type, 25°C, pH 7.5 | Escherichia coli | |
2.5.1.31 | 0.0007 | - |
geranylgeranyl diphosphate | pH 7.5, 25°C, mutant L88A | Escherichia coli | |
2.5.1.31 | 0.0007 | - |
geranylgeranyl diphosphate | L88A mutant, 25°C, pH 7.5 | Escherichia coli | |
2.5.1.31 | 0.001 | - |
geranylgeranyl diphosphate | pH 7.5, 25°C, mutant F89A | Escherichia coli | |
2.5.1.31 | 0.001 | - |
geranylgeranyl diphosphate | F89A mutant, 25°C, pH 7.5 | Escherichia coli | |
2.5.1.31 | 0.0012 | - |
geranylgeranyl diphosphate | pH 7.5, 25°C, mutant L85A | Escherichia coli | |
2.5.1.31 | 0.0012 | - |
geranylgeranyl diphosphate | L85A mutant, 25°C, pH 7.5 | Escherichia coli | |
2.5.1.31 | 0.0016 | - |
farnesyl diphosphate | pH 7.5, 25°C, mutant L85A | Escherichia coli | |
2.5.1.31 | 0.0016 | - |
(2E,6E)-farnesyl diphosphate | L85A mutant, 25°C, pH 7.5 | Escherichia coli | |
2.5.1.31 | 0.0021 | - |
farnesyl diphosphate | pH 7.5, 25°C, mutant L88A | Escherichia coli | |
2.5.1.31 | 0.0021 | - |
(2E,6E)-farnesyl diphosphate | L88A mutant, 25°C, pH 7.5 | Escherichia coli | |
2.5.1.31 | 0.0026 | - |
farnesyl diphosphate | pH 7.5, 25°C, mutant F89A | Escherichia coli | |
2.5.1.31 | 0.0026 | - |
(2E,6E)-farnesyl diphosphate | F89A mutant, 25°C, pH 7.5 | Escherichia coli | |
2.5.1.31 | 0.0041 | - |
isopentenyl diphosphate | pH 7.5, 25°C, wild-type enzyme, with farnesyl diphosphate | Escherichia coli | |
2.5.1.31 | 0.0041 | - |
isopentenyl diphosphate | wild type, with (2E,6E)-farnesyl diphosphate, 25°C, pH 7.5 | Escherichia coli | |
2.5.1.31 | 0.013 | - |
isopentenyl diphosphate | pH 7.5, 25°C, wild-type enzyme, with geranylgeranyl diphosphate | Escherichia coli | |
2.5.1.31 | 0.013 | - |
isopentenyl diphosphate | wild type, with geranylgeranyl diphosphate, 25°C, pH 7.5 | Escherichia coli | |
2.5.1.31 | 0.018 | - |
isopentenyl diphosphate | pH 7.5, 25°C, mutant F89A, with geranylgeranyl diphosphate | Escherichia coli | |
2.5.1.31 | 0.018 | - |
isopentenyl diphosphate | F89A mutant, with geranylgeranyl diphosphate, 25°C, pH 7.5 | Escherichia coli | |
2.5.1.31 | 0.036 | - |
geranyl diphosphate | 25°C, pH 7.5 | Escherichia coli | |
2.5.1.31 | 0.042 | - |
isopentenyl diphosphate | pH 7.5, 25°C, mutant L88A, with geranylgeranyl diphosphate | Escherichia coli | |
2.5.1.31 | 0.042 | - |
isopentenyl diphosphate | L88A mutant, with geranylgeranyl diphosphate, 25°C, pH 7.5 | Escherichia coli | |
2.5.1.31 | 0.06 | - |
isopentenyl diphosphate | pH 7.5, 25°C, mutant L85A, with geranylgeranyl diphosphate | Escherichia coli | |
2.5.1.31 | 0.06 | - |
isopentenyl diphosphate | L85A mutant, with geranylgeranyl diphosphate, 25°C, pH 7.5 | Escherichia coli | |
2.5.1.31 | 0.084 | - |
isopentenyl diphosphate | pH 7.5, 25°C, mutant F89A, with farnesyl diphosphate | Escherichia coli | |
2.5.1.31 | 0.084 | - |
isopentenyl diphosphate | F89A mutant, with (2E,6E)-farnesyl diphosphate, 25°C, pH 7.5 | Escherichia coli | |
2.5.1.31 | 0.173 | - |
isopentenyl diphosphate | pH 7.5, 25°C, mutant L88A, with farnesyl diphosphate | Escherichia coli | |
2.5.1.31 | 0.173 | - |
isopentenyl diphosphate | L88A mutant, with (2E,6E)-farnesyl diphosphate, 25°C, pH 7.5 | Escherichia coli | |
2.5.1.31 | 0.617 | - |
isopentenyl diphosphate | pH 7.5, 25°C, mutant L85A, with farnesyl diphosphate | Escherichia coli | |
2.5.1.31 | 0.617 | - |
isopentenyl diphosphate | L85A mutant, with (2E,6E)-farnesyl diphosphate, 25°C, pH 7.5 | Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.31 | Mg2+ | - |
Escherichia coli |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.31 | farnesyl diphosphate + isopentenyl diphosphate | Escherichia coli | consecutive condensation of 8 molecules of isopentenyl diphosphate with farnesyl diphosphate to form the lipid carrier undecaprenyl diphosphate to mediate bacterial peptidoglycan synthesis, the final product has C55-C60 chain length, molecular 3D-structural modeling of the reaction product | diphosphate + di-trans-poly-cis-undecaprenyl diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.31 | Escherichia coli | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.31 | (2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate | - |
Escherichia coli | 8 diphosphate + ditrans,octacis-undecaprenyl diphosphate | - |
? | |
2.5.1.31 | farnesyl diphosphate + isopentenyl diphosphate | consecutive condensation of 8 molecules of isopentenyl diphosphate with farnesyl diphosphate to form the lipid carrier undecaprenyl diphosphate to mediate bacterial peptidoglycan synthesis, the final product has C55-C60 chain length, molecular 3D-structural modeling of the reaction product | Escherichia coli | diphosphate + di-trans-poly-cis-undecaprenyl diphosphate | - |
? | |
2.5.1.31 | farnesyl diphosphate + isopentenyl diphosphate | the phosphate head group of farnesyl diphosphate is bound to the positively charged Arg residues and the hydrocarbon moiety interacts with hydrophobic amino acids including L85, L88, and F89, located on the alpha3-helix of the enzyme | Escherichia coli | diphosphate + di-trans-poly-cis-undecaprenyl diphosphate | - |
? | |
2.5.1.31 | farnesyl phosphate + isopentenyl diphosphate | low activity | Escherichia coli | phosphate + di-trans-poly-cis-undecaprenyl diphosphate | - |
? | |
2.5.1.31 | geranyl diphosphate + isopentenyl diphosphate | less effective than farnesyl diphosphate | Escherichia coli | diphosphate + ? | - |
? | |
2.5.1.31 | geranyl diphosphate + isopentenyl diphosphate | C10 GPP displays a 90fold larger Km value compared with (2E,6E)-farnesyl diphosphate | Escherichia coli | ? | - |
? | |
2.5.1.31 | geranylgeranyl diphosphate + isopentenyl diphosphate | as effective as farnesyl diphosphate | Escherichia coli | diphosphate + ? | - |
? | |
2.5.1.31 | geranylgeranyl diphosphate + isopentenyl diphosphate | containing a larger C20 hydrocarbon tail, is an equally good substrate | Escherichia coli | ? | - |
? | |
2.5.1.31 | isopentenyl diphosphate + isopentenyl diphosphate | - |
Escherichia coli | ? | - |
? | |
2.5.1.31 | additional information | role of the hydrocarbon moiety of the allylic substrate in the reaction | Escherichia coli | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.31 | cis-type undecaprenyl pyrophosphate synthase | - |
Escherichia coli |
2.5.1.31 | More | cis-type enzyme | Escherichia coli |
2.5.1.31 | undecaprenyl pyrophosphate synthase | - |
Escherichia coli |
2.5.1.31 | UPPs | - |
Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.5.1.31 | 25 | - |
assay at | Escherichia coli |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.31 | 0.095 | - |
farnesyl diphosphate | pH 7.5, 25°C, mutant L85A | Escherichia coli | |
2.5.1.31 | 0.095 | - |
(2E,6E)-farnesyl diphosphate | L85A mutant, 25°C, pH 7.5 | Escherichia coli | |
2.5.1.31 | 0.18 | - |
geranylgeranyl diphosphate | pH 7.5, 25°C, mutant L85A | Escherichia coli | |
2.5.1.31 | 0.18 | - |
geranylgeranyl diphosphate | L85A mutant, 25°C, pH 7.5 | Escherichia coli | |
2.5.1.31 | 0.23 | - |
farnesyl diphosphate | pH 7.5, 25°C, mutant L88A | Escherichia coli | |
2.5.1.31 | 0.23 | - |
(2E,6E)-farnesyl diphosphate | L88A mutant, 25°C, pH 7.5 | Escherichia coli | |
2.5.1.31 | 0.32 | - |
geranylgeranyl diphosphate | pH 7.5, 25°C, mutant L88A | Escherichia coli | |
2.5.1.31 | 0.32 | - |
geranylgeranyl diphosphate | L88A mutant, 25°C, pH 7.5 | Escherichia coli | |
2.5.1.31 | 0.61 | - |
farnesyl diphosphate | pH 7.5, 25°C, mutant F89A | Escherichia coli | |
2.5.1.31 | 0.61 | - |
(2E,6E)-farnesyl diphosphate | F89A mutant, 25°C, pH 7.5 | Escherichia coli | |
2.5.1.31 | 0.7 | - |
geranylgeranyl diphosphate | pH 7.5, 25°C, mutant F89A | Escherichia coli | |
2.5.1.31 | 0.7 | - |
geranylgeranyl diphosphate | F89A mutant, 25°C, pH 7.5 | Escherichia coli | |
2.5.1.31 | 1.7 | - |
geranyl diphosphate | 25°C, pH 7.5 | Escherichia coli | |
2.5.1.31 | 2.1 | - |
geranylgeranyl diphosphate | pH 7.5, 25°C, wild-type enzyme | Escherichia coli | |
2.5.1.31 | 2.1 | - |
geranylgeranyl diphosphate | wild type, 25°C, pH 7.5 | Escherichia coli | |
2.5.1.31 | 2.5 | - |
farnesyl diphosphate | pH 7.5, 25°C, wild-type enzyme | Escherichia coli | |
2.5.1.31 | 2.5 | - |
(2E,6E)-farnesyl diphosphate | wild type, 25°C, pH 7.5 | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.31 | 7.5 | - |
assay at | Escherichia coli |