EC Number | Cloned (Comment) | Organism |
---|---|---|
1.13.11.50 | DNA and amino acid sequence determination and analysis | Acinetobacter johnsonii |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.13.11.50 | - |
Acinetobacter johnsonii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.7.1.7 | PMSF | completely inhibits | Pseudomonas sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.50 | 0.0091 | - |
acetylacetate | - |
Acinetobacter johnsonii |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.50 | Iron | dependent on, metalloenzyme, 1 iron bound per subunit, required for positioning of the substrate and for rendering of the appropriate electronic environment | Acinetobacter johnsonii | |
3.7.1.2 | Ca2+ | - |
Mus musculus | |
3.7.1.2 | Mg2+ | stabilizes the beta-roll that constitutes the active site of the enzyme | Mus musculus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.13.11.50 | 16000 | - |
4 * 16000 | Acinetobacter johnsonii |
3.7.1.2 | 46000 | - |
2 * 46000, method not mentioned | Mus musculus |
3.7.1.7 | 40000 | - |
- |
Pseudomonas sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.50 | pentane-2,4-dione + O2 | Acinetobacter johnsonii | - |
acetate + 2-oxopropanal | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.50 | Acinetobacter johnsonii | - |
- |
- |
3.7.1.2 | Bos taurus | - |
- |
- |
3.7.1.2 | Mus musculus | - |
- |
- |
3.7.1.7 | Pseudomonas sp. | - |
- |
- |
3.7.1.7 | Pseudomonas sp. VM15C | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.13.11.50 | pentane-2,4-dione + O2 = acetate + 2-oxopropanal | reaction mechanism | Acinetobacter johnsonii |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.13.11.50 | culture condition:acetylacetone-grown cell | as sole carbon source | Acinetobacter johnsonii | - |
3.7.1.2 | liver | - |
Bos taurus | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.50 | 1-phenylbutane-1,3-dione + O2 | - |
Acinetobacter johnsonii | ? | - |
? | |
1.13.11.50 | 2-acetylcyclohexanone + O2 | - |
Acinetobacter johnsonii | ? | - |
? | |
1.13.11.50 | 3-oxobutanone + O2 | - |
Acinetobacter johnsonii | ? | - |
? | |
1.13.11.50 | additional information | the catalytic reaction proceeds via one common rate-limiting step in the clevage of all substrates accepted by the enzyme | Acinetobacter johnsonii | ? | - |
? | |
1.13.11.50 | pentane-2,4-dione + O2 | - |
Acinetobacter johnsonii | acetate + 2-oxopropanal | - |
? | |
1.13.11.50 | pentane-2,4-dione + O2 | i.e. acetylacetone | Acinetobacter johnsonii | acetate + 2-oxopropanal | i.e. acetate and acetone | ? | |
3.7.1.2 | 4-fumarylacetoacetate + H2O | - |
Mus musculus | acetoacetate + fumarate | - |
? | |
3.7.1.2 | 4-fumarylacetoacetate + H2O | - |
Bos taurus | acetoacetate + fumarate | - |
? | |
3.7.1.2 | acetylpyruvate + H2O | - |
Bos taurus | ? | - |
? | |
3.7.1.7 | 4,6-nonanedione + H2O | - |
Pseudomonas sp. | butyric acid + 2-pentanone | - |
? | |
3.7.1.7 | 4,6-nonanedione + H2O | - |
Pseudomonas sp. VM15C | butyric acid + 2-pentanone | - |
? | |
3.7.1.7 | additional information | no activity towards pentane-2,4-dione | Pseudomonas sp. | ? | - |
? | |
3.7.1.7 | additional information | no activity towards pentane-2,4-dione | Pseudomonas sp. VM15C | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.13.11.50 | tetramer | 4 * 16000 | Acinetobacter johnsonii |
3.7.1.2 | dimer | 2 * 46000, method not mentioned | Mus musculus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.13.11.50 | diketone-cleaving enzyme | - |
Acinetobacter johnsonii |
1.13.11.50 | Dke1 | - |
Acinetobacter johnsonii |
1.13.11.50 | pentane-2,4-dione hydrolase | - |
Acinetobacter johnsonii |
3.7.1.2 | FAH | - |
Mus musculus |
3.7.1.2 | FAH | - |
Bos taurus |
3.7.1.2 | fumarylacetoacetate hydrolase | - |
Mus musculus |
3.7.1.2 | fumarylacetoacetate hydrolase | - |
Bos taurus |
3.7.1.7 | OPH hydrolase | - |
Pseudomonas sp. |
3.7.1.7 | oxidized polyvinyl alcohol hydrolase | - |
Pseudomonas sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.50 | 8.5 | - |
acetylacetate | - |
Acinetobacter johnsonii |