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Literature summary extracted from

  • Link, T.; Lohaus, G.; Heiser, I.; Mendgen, K.; Hahn, M.; Voegele, R.T.
    Characterization of a novel NADP(+)-dependent D-arabitol dehydrogenase from the plant pathogen Uromyces fabae (2005), Biochem. J., 389, 289-295.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.287 gene ARD1, DNA and amino acid sequence determination and analysis, expression in Saccharomyces cerevisiae strain 23344c, overexpression in Escherichia coli strain BL21(DE3) as N-terminally His6-tagged protein Uromyces viciae-fabae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.287 additional information
-
 additional information kinetics Uromyces viciae-fabae
1.1.1.287 0.07
-
 NADP+ pH 9.0, with D-mannitol Uromyces viciae-fabae
1.1.1.287 0.08
-
 NADP+ pH 9.0, with D-arabitinol Uromyces viciae-fabae
1.1.1.287 0.16
-
 NADPH pH 6.0, with D-fructose Uromyces viciae-fabae
1.1.1.287 0.2
-
 NADPH pH 6.0, with D-ribulose Uromyces viciae-fabae
1.1.1.287 0.26
-
 NADPH pH 6.0, with D-xylulose Uromyces viciae-fabae
1.1.1.287 7.8
-
 D-xylulose pH 6.0 Uromyces viciae-fabae
1.1.1.287 53.9
-
 D-ribulose pH 6.0 Uromyces viciae-fabae
1.1.1.287 160
-
 D-fructose pH 6.0 Uromyces viciae-fabae
1.1.1.287 425
-
 D-mannitol pH 9.0 Uromyces viciae-fabae
1.1.1.287 834
-
 D-arabitinol pH 9.0 Uromyces viciae-fabae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.287 soluble
-
 Uromyces viciae-fabae
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.287 additional information Uromyces viciae-fabae the enzyme activity is highly increased during infection processes and pathogenesis, development after infection, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.287 Uromyces viciae-fabae Q4R0J7 cultivated on artificial surfaces in in vitro infection structures
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.287 recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Uromyces viciae-fabae

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.287 haustorium exclusively, in the lumen Uromyces viciae-fabae
-
1.1.1.287 additional information cultivated on artificial surfaces in in vitro infection structures Uromyces viciae-fabae
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.287 D-arabitinol + NADP+
-
 Uromyces viciae-fabae D-xylulose + NADPH + H+
-
 r
1.1.1.287 D-arabitinol + NADP+
-
 Uromyces viciae-fabae D-ribulose + NADPH + H+
-
 r
1.1.1.287 D-mannitol + NADP+
-
 Uromyces viciae-fabae D-fructose + NADPH + H+
-
 r
1.1.1.287 additional information the enzyme activity is highly increased during infection processes and pathogenesis, development after infection, overview Uromyces viciae-fabae ?
-
 ?
1.1.1.287 additional information the enzyme shows high substrate specificity, the reverse reaction is preferred Uromyces viciae-fabae ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.287 ARD1p
-
 Uromyces viciae-fabae
1.1.1.287 D-arabitol dehydrogenase 1
-
 Uromyces viciae-fabae
1.1.1.287 NADP(+)-dependent D-arabitol dehydrogenase
-
 Uromyces viciae-fabae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.287 6
-
 assay at, reverse reaction Uromyces viciae-fabae
1.1.1.287 9
-
 assay at, forward reaction Uromyces viciae-fabae

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.287 NADP+ dependent on, absolutely specific for, forward reaction Uromyces viciae-fabae
1.1.1.287 NADPH dependent on, absolutely specific for, reverse reaction Uromyces viciae-fabae