Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bennett, E.J.; Bjerregaard, J.; Knapp, J.E.; Chavous, D.A.; Friedman, A.M.; Royer, W.E., Jr.; O'Connor, C.M.
    Catalytic implications from the Drosophila protein L-isoaspartyl methyltransferase structure and site-directed mutagenesis (2003), Biochemistry, 42, 12844-12853.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.77 overexpression in transgenic flies extends the normal life span, suggesting that protein damage can be a limiting factor in longevity Drosophila melanogaster

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.77 sitting drop vapour diffusion method, resolution of 2.2 A Drosophila melanogaster

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.77 S60A inactive mutant protein Drosophila melanogaster
2.1.1.77 S60Q the ratio of turnover number to KM-value for ovalbumin L-isoaspartate is 24.3fold lower than the wild-type value, the ratio of turnover-number to KM-value for KASA(iso-D)LAKY is 21fold lower than wild-type value Drosophila melanogaster
2.1.1.77 S60T the ratio of turnover number to KM-value for ovalbumin L-isoaspartate is 6.2fold lower than the wild-type value, the ratio of turnover-number to KM-value for KASA(iso-D)LAKY is 2fold lower than the wild-type value Drosophila melanogaster

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.77 0.0145
-
 KASA(iso-D)LAKY wild-type enzyme Drosophila melanogaster
2.1.1.77 0.023
-
 KASA(iso-D)LAKY mutant enzyme S60Q Drosophila melanogaster
2.1.1.77 0.0286
-
 KASA(iso-D)LAKY mutant enzyme S60T Drosophila melanogaster
2.1.1.77 0.0423
-
 ovalbumin L-isoaspartate mutant enzyme S60Q Drosophila melanogaster
2.1.1.77 0.121
-
 ovalbumin L-isoaspartate wild-type enzyme Drosophila melanogaster
2.1.1.77 0.159
-
 ovalbumin L-isoaspartate mutant enzyme S60T Drosophila melanogaster

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate Drosophila melanogaster the enzyme catalyzes the methylation of L-isoaspartyl residues that arise spontaneously in proteins with age, thereby initiating a repair process that restores the normal backbone configuration to the damaged polypeptide S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.77 Drosophila melanogaster Q27869
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.77
-
 Drosophila melanogaster

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.77 S-adenosyl-L-methionine + KASA(iso-D)LAKY
-
 Drosophila melanogaster S-adenosyl-L-homocysteine + KASA(isoaspartate alpha-methyl ester)LAKY
-
 ?
2.1.1.77 S-adenosyl-L-methionine + KASA(iso-D)LAKY
-
 Drosophila melanogaster S-adenosyl-L-homocysteine + KASA(L-isoaspartate alpha-methyl ester)LAKY
-
 ?
2.1.1.77 S-adenosyl-L-methionine + ovalbumin L-isoaspartate
-
 Drosophila melanogaster S-adenosyl-L-homocysteine + ovalbumin L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + protein L-isoaspartate the enzyme catalyzes the methylation of L-isoaspartyl residues that arise spontaneously in proteins with age, thereby initiating a repair process that restores the normal backbone configuration to the damaged polypeptide Drosophila melanogaster S-adenosyl-L-homocysteine + protein L-isoaspartate alpha-methyl ester
-
 ?
2.1.1.77 S-adenosyl-L-methionine + VYP(iso-D)HA
-
 Drosophila melanogaster S-adenosyl-L-homocysteine + VYP(isoaspartate alpha-methyl ester)HA
-
 ?
2.1.1.77 S-adenosyl-L-methionine + VYP(iso-D)HA
-
 Drosophila melanogaster S-adenosyl-L-homocysteine + VYP(L-isoaspartate alpha-methyl ester)HA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.77 PIMT
-
 Drosophila melanogaster
2.1.1.77 protein L-isoaspartyl methyltransferase
-
 Drosophila melanogaster