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Literature summary extracted from

  • Suharti; Heering, H.A.; de Vries, S.
    NO reductase from Bacillus azotoformans is a bifunctional enzyme accepting electrons from menaquinol and a specific endogenous membrane-bound cytochrome c551 (2004), Biochemistry, 43, 13487-13495.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.2.5 cytochrome c
-
Schinkia azotoformans
1.7.5.2 cytochrome c551 noncompetitive inhibitor of nitric oxide reduction when menaquinol is used as an electron donor Schinkia azotoformans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.2.5 0.14
-
5-hydroxy-1,4-naphthoquinone
-
Schinkia azotoformans
1.7.2.5 0.26
-
5-hydroxy-2-methyl-1,4-naphthoquinol
-
Schinkia azotoformans
1.7.5.2 0.0045
-
cytochrome c551 pH and temperature not specified in the publication Schinkia azotoformans
1.7.5.2 0.141
-
juglone pH and temperature not specified in the publication Schinkia azotoformans
1.7.5.2 0.256
-
Plumbagin pH and temperature not specified in the publication Schinkia azotoformans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.2.5 copper contains CuA in the small subunit Schinkia azotoformans
1.7.5.2 copper the enzyme contains a CuA center in its small subunit Schinkia azotoformans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.2.5 NO + reduced acceptor Schinkia azotoformans
-
N2O + H2O + acceptor
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.5 Schinkia azotoformans
-
-
-
1.7.5.2 Schinkia azotoformans
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.2.5 NO + 5-hydroxy-1,4-naphthoquinol
-
Schinkia azotoformans N2O + H2O + 5-hydroxy-1,4-naphthoquinone
-
?
1.7.2.5 NO + 5-hydroxy-2-methyl-1,4-naphthoquinol
-
Schinkia azotoformans N2O + H2O + 5-hydroxy-2-methyl-1,4-naphthoquinone
-
?
1.7.2.5 NO + menaquinol
-
Schinkia azotoformans N2O + H2O + menaquinone
-
?
1.7.2.5 NO + reduced acceptor
-
Schinkia azotoformans N2O + H2O + acceptor
-
?
1.7.2.5 NO + reduced cytochrome c551
-
Schinkia azotoformans N2O + H2O + oxidized cytochrome c551
-
?
1.7.5.2 additional information no activity with lapachol, reduced horse heart cytochrome c, cytochrome c550, and cytochrome c552 Schinkia azotoformans ?
-
?
1.7.5.2 nitric oxide + cytochrome c551 the menaquinol pathway has a 4fold greater maximal activity than the pathway via cytochrome c551 Schinkia azotoformans ?
-
?
1.7.5.2 nitric oxide + juglone
-
Schinkia azotoformans ?
-
?
1.7.5.2 nitric oxide + menaquinol the menaquinol pathway has a 4fold greater maximal activity than the pathway via cytochrome c551 Schinkia azotoformans nitrous oxide + menaquinone + H2O
-
?
1.7.5.2 nitric oxide + plumbagin
-
Schinkia azotoformans ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.7.2.5 qCuANOR
-
Schinkia azotoformans
1.7.5.2 menaquinol-dependent nitric oxide reductase
-
Schinkia azotoformans
1.7.5.2 NO reductase
-
Schinkia azotoformans
1.7.5.2 qCuANOR
-
Schinkia azotoformans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7.2.5 118
-
NO with 5-hydroxy-1,4-naphthoquinol Schinkia azotoformans
1.7.2.5 150
-
NO with 5-hydroxy-2-methyl-1,4-naphthoquinol Schinkia azotoformans
1.7.5.2 41
-
nitric oxide using cytochrome c551 as electron donor, pH and temperature not specified in the publication Schinkia azotoformans
1.7.5.2 118
-
nitric oxide using juglone as electron donor, pH and temperature not specified in the publication Schinkia azotoformans
1.7.5.2 150
-
nitric oxide using plumbagin as electron donor, pH and temperature not specified in the publication Schinkia azotoformans
1.7.5.2 153
-
nitric oxide using menaquinol as electron donor, pH and temperature not specified in the publication Schinkia azotoformans

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.7.2.5 0.005
-
cytochrome c
-
Schinkia azotoformans

General Information

EC Number General Information Comment Organism
1.7.5.2 physiological function the ability of qCuANOR to accept electrons from both menaquinol and cytochrome c551 may be related to the regulation of the rate of NO reduction especially as a defense mechanism of B. azotoformans against the toxicity of NO. The menaquinol pathway, which has a 4-fold greater maximal activity than the pathway via cytochrome c551, is used for NO detoxification, whereas electron donation via the endogenous cytochrome c involves the cytochrome b6f complex serving the bioenergetic needs of the organism Schinkia azotoformans