EC Number | Cloned (Comment) | Organism |
---|---|---|
1.18.1.2 | expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Anabaena sp. |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.18.1.2 | Y303F | site-directed mutagenesis, about 30% of the wild-type enzyme activity with ferredoxin, about 25% of the wild-type enzyme activity with flavodoxin | Anabaena sp. |
1.18.1.2 | Y303S | site-directed mutagenesis, inactive mutant | Anabaena sp. |
1.18.1.2 | Y303W | site-directed mutagenesis, nearly inactive mutant | Anabaena sp. |
1.18.1.2 | Y308F | site-directed mutagenesis, about 20% of the wild-type enzyme activity with ferredoxin, about 11% of the wild-type enzyme activity with flavodoxin | Pisum sativum |
1.18.1.2 | Y308S | site-directed mutagenesis, about 5% of the wild-type enzyme activity with ferredoxin, no activity with flavodoxin | Pisum sativum |
1.18.1.2 | Y308W | site-directed mutagenesis, nearly inactive mutant with ferredoxin, about 25% of the wild-type enzyme activity with flavodoxin | Pisum sativum |
1.19.1.1 | Y303F | about 30% of the wild-type enzyme activity with ferredoxin, about 25% of the wild-type enzyme activity with flavodoxin | Nostoc sp. |
1.19.1.1 | Y303S | inactive. Mutation shifts the flavin reduction potential to less negative values, whereas semiquinone stabilization is severely hampered | Nostoc sp. |
1.19.1.1 | Y303W | almost inactive | Nostoc sp. |
1.19.1.1 | Y308F | about 20% of the wild-type enzyme activity with ferredoxin, about 11% of the wild-type enzyme activity with flavodoxin | Pisum sativum |
1.19.1.1 | Y308S | nearly inactive mutant with ferredoxin, about 25% of the wild-type enzyme activity with flavodoxin. Mutation shifts the flavin reduction potential to less negative values, whereas semiquinone stabilization is severely hampered | Pisum sativum |
1.19.1.1 | Y308W | about 5% of the wild-type enzyme activity with ferredoxin, no activity with flavodoxin | Pisum sativum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.18.1.2 | additional information | - |
additional information | stopped-flow and laser flash kinetic measurements, steady-state kinetics, dissociation constants and reduction potentials of wild-type and mutant enzymes | Pisum sativum | |
1.18.1.2 | additional information | - |
additional information | stopped-flow and laser flash kinetic measurements, steady-state kinetics, dissociation constants and reduction potentials of wild-type and mutant enzymes | Anabaena sp. | |
1.18.1.2 | 0.0058 | - |
reduced ferredoxin | pH 8.0, 13°C, mutant Y308F | Pisum sativum | |
1.18.1.2 | 0.0065 | - |
reduced ferredoxin | pH 8.0, 13°C, wild-type enzyme | Pisum sativum | |
1.18.1.2 | 0.009 | - |
reduced ferredoxin | pH 8.0, 13°C, mutant Y308S | Pisum sativum | |
1.18.1.2 | 0.011 | - |
reduced ferredoxin | pH 8.0, 13°C, wild-type enzyme | Anabaena sp. | |
1.18.1.2 | 0.017 | - |
reduced ferredoxin | pH 8.0, 13°C, mutant Y308W | Pisum sativum | |
1.18.1.2 | 0.051 | - |
reduced ferredoxin | pH 8.0, 13°C, mutant Y303F | Anabaena sp. | |
1.19.1.1 | 0.0167 | - |
reduced flavodoxin | wild-type, pH 8.0, 25°C | Pisum sativum | |
1.19.1.1 | 0.017 | - |
reduced flavodoxin | mutant Y308W, pH 8.0, 25°C | Pisum sativum | |
1.19.1.1 | 0.02 | - |
reduced flavodoxin | mutant Y308F, pH 8.0, 25°C | Pisum sativum | |
1.19.1.1 | 0.033 | - |
reduced flavodoxin | wild-type, pH 8.0, 25°C | Nostoc sp. | |
1.19.1.1 | 0.043 | - |
reduced flavodoxin | mutant Y303F, pH 8.0, 25°C | Nostoc sp. |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.19.1.1 | chloroplast | - |
Pisum sativum | 9507 | - |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.18.1.2 | Anabaena sp. | P21890 | - |
- |
1.18.1.2 | Pisum sativum | - |
- |
- |
1.19.1.1 | Nostoc sp. | P21890 | enzyme catalyzes reactions of both EC 1.18.1.2 and EC 1.19.1.1 | - |
1.19.1.1 | Nostoc sp. ATCC 29151 | P21890 | enzyme catalyzes reactions of both EC 1.18.1.2 and EC 1.19.1.1 | - |
1.19.1.1 | Pisum sativum | P10933 | enzyme catalyzes reactions of both EC 1.18.1.2 and EC 1.19.1.1 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.18.1.2 | recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3) | Anabaena sp. |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
1.18.1.2 | 2 reduced ferredoxin + NADP+ + H+ = 2 oxidized ferredoxin + NADPH | catalytic mechanism, initiated by reduction of FAD cofactor by obligatory one-electron carriers ferredoxin or flavodoxin in presence of NADP+, the enzymes' C-terminal tyrosine residue is involved modulating the enzyme affinity for NADP+/NADPH | Pisum sativum | |
1.18.1.2 | 2 reduced ferredoxin + NADP+ + H+ = 2 oxidized ferredoxin + NADPH | catalytic mechanism, initiated by reduction of FAD cofactor by obligatory one-electron carriers ferredoxin or flavodoxin in presence of NADP+, the enzymes' C-terminal tyrosine residue is involved modulating the enzyme affinity for NADP+/NADPH | Anabaena sp. |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.19.1.1 | leaf | - |
Pisum sativum | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.18.1.2 | reduced ferredoxin + NADP+ | - |
Pisum sativum | oxidized ferredoxin + NADPH | - |
r | |
1.18.1.2 | reduced ferredoxin + NADP+ | - |
Anabaena sp. | oxidized ferredoxin + NADPH | - |
r | |
1.18.1.2 | reduced ferredoxin + NADP+ | - |
Pisum sativum | oxidized ferredoxin + NADPH + H+ | - |
r | |
1.18.1.2 | reduced ferredoxin + NADP+ | - |
Anabaena sp. | oxidized ferredoxin + NADPH + H+ | - |
r | |
1.18.1.2 | reduced flavodoxin + NADP+ | - |
Pisum sativum | oxidized flavodoxin + NADPH + H+ | - |
r | |
1.18.1.2 | reduced flavodoxin + NADP+ | - |
Anabaena sp. | oxidized flavodoxin + NADPH + H+ | - |
r | |
1.19.1.1 | reduced flavodoxin + NADP+ | - |
Pisum sativum | oxidized flavodoxin + NADPH + H+ | - |
? | |
1.19.1.1 | reduced flavodoxin + NADP+ | - |
Nostoc sp. | oxidized flavodoxin + NADPH + H+ | - |
? | |
1.19.1.1 | reduced flavodoxin + NADP+ | - |
Nostoc sp. ATCC 29151 | oxidized flavodoxin + NADPH + H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.19.1.1 | More | the C-terminal tyrosine residue lowers the affinity for NADP(H) to levels compatible with steady-state turnover, contributes to the flavin semiquinone stabilization required for electron splitting, and modulates the rates of electron exchange with the protein partners | Pisum sativum |
1.19.1.1 | More | the C-terminal tyrosine residue lowers the affinity for NADP(H) to levels compatible with steady-state turnover, contributes to the flavin semiquinone stabilization required for electron splitting, and modulates the rates of electron exchange with the protein partners | Nostoc sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.18.1.2 | ferredoxin-nicotinamide adenine dinucleotide phosphate reductase | - |
Pisum sativum |
1.18.1.2 | ferredoxin-nicotinamide adenine dinucleotide phosphate reductase | - |
Anabaena sp. |
1.18.1.2 | FNR | - |
Pisum sativum |
1.18.1.2 | FNR | - |
Anabaena sp. |
1.19.1.1 | PETH | - |
Pisum sativum |
1.19.1.1 | PETH | - |
Nostoc sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.18.1.2 | 13 | - |
assay at | Pisum sativum |
1.18.1.2 | 13 | - |
assay at | Anabaena sp. |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.18.1.2 | 1 | - |
reduced ferredoxin | pH 8.0, 13°C, mutant Y303W | Anabaena sp. | |
1.18.1.2 | 2.5 | - |
reduced flavodoxin | pH 8.0, 13°C, mutant Y303W | Anabaena sp. | |
1.18.1.2 | 2.5 | - |
reduced ferredoxin | pH 8.0, 13°C, mutant Y308W | Pisum sativum | |
1.18.1.2 | 4 | - |
reduced flavodoxin | pH 8.0, 13°C, mutant Y308F | Pisum sativum | |
1.18.1.2 | 7 | - |
reduced flavodoxin | pH 8.0, 13°C, mutant Y303F | Anabaena sp. | |
1.18.1.2 | 7.7 | - |
reduced ferredoxin | pH 8.0, 13°C, mutant Y308S | Pisum sativum | |
1.18.1.2 | 8.3 | - |
reduced flavodoxin | pH 8.0, 13°C, mutant Y308W | Pisum sativum | |
1.18.1.2 | 23.3 | - |
reduced flavodoxin | pH 8.0, 13°C, wild-type enzyme | Anabaena sp. | |
1.18.1.2 | 23.9 | - |
reduced ferredoxin | pH 8.0, 13°C, mutant Y308F | Pisum sativum | |
1.18.1.2 | 30.6 | - |
reduced flavodoxin | pH 8.0, 13°C, wild-type enzyme | Pisum sativum | |
1.18.1.2 | 32 | - |
reduced ferredoxin | pH 8.0, 13°C, mutant Y303F | Anabaena sp. | |
1.18.1.2 | 139 | - |
reduced ferredoxin | pH 8.0, 13°C, wild-type enzyme | Pisum sativum | |
1.18.1.2 | 200 | - |
reduced ferredoxin | pH 8.0, 13°C, wild-type enzyme | Anabaena sp. | |
1.19.1.1 | 2.5 | - |
reduced flavodoxin | mutant Y303W, pH 8.0, 25°C | Nostoc sp. | |
1.19.1.1 | 4 | - |
reduced flavodoxin | mutant Y308F, pH 8.0, 25°C | Pisum sativum | |
1.19.1.1 | 7 | - |
reduced flavodoxin | mutant Y303F, pH 8.0, 25°C | Nostoc sp. | |
1.19.1.1 | 8.3 | - |
reduced flavodoxin | mutant Y308W, pH 8.0, 25°C | Pisum sativum | |
1.19.1.1 | 23.3 | - |
reduced flavodoxin | wild-type, pH 8.0, 25°C | Nostoc sp. | |
1.19.1.1 | 30.6 | - |
reduced flavodoxin | wild-type, pH 8.0, 25°C | Pisum sativum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.18.1.2 | 8 | - |
assay at | Pisum sativum |
1.18.1.2 | 8 | - |
assay at | Anabaena sp. |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.18.1.2 | FAD | binding and hydride/electron transfer mechanism | Pisum sativum | |
1.18.1.2 | FAD | binding and hydride/electron transfer mechanism | Anabaena sp. | |
1.18.1.2 | NADP+ | binding and hydride transfer mechanism | Pisum sativum | |
1.18.1.2 | NADP+ | binding and hydride transfer mechanism | Anabaena sp. | |
1.18.1.2 | NADPH | binding and hydride transfer mechanism | Pisum sativum | |
1.18.1.2 | NADPH | binding and hydride transfer mechanism | Anabaena sp. |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.19.1.1 | 170 | - |
reduced flavodoxin | mutant Y303F, pH 8.0, 25°C | Nostoc sp. | |
1.19.1.1 | 200 | - |
reduced flavodoxin | mutant Y308F, pH 8.0, 25°C | Pisum sativum | |
1.19.1.1 | 500 | - |
reduced flavodoxin | mutant Y308W, pH 8.0, 25°C | Pisum sativum | |
1.19.1.1 | 700 | - |
reduced flavodoxin | wild-type, pH 8.0, 25°C | Nostoc sp. | |
1.19.1.1 | 1800 | - |
reduced flavodoxin | wild-type, pH 8.0, 25°C | Pisum sativum |