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Literature summary extracted from

  • Karasyova, E.I.; Naumchik, I.V.; Metelitza, D.I.
    Activation of peroxidase-catalyzed oxidation of aromatic amines with 2-aminothiazole and melamine (2003), Biochemistry, 68, 54-62.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.11.1.7 2-aminothiazole 2 mM, 4fold, more than 2fold and 1.4fold activation of 3,3',5,5'-tetramethylbenzidine, o-phenylenediamine and 5-aminosalycylic acid oxidation, respectively Armoracia rusticana
1.11.1.7 melamine 1 mM, approx. 2fold increase in kcat for oxidation of 3,3',5,5'-tetramethylbenzidine Armoracia rusticana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.7 2,4-dinitroresorcinol competitive inhibition of 3,3',5,5'-tetramethylbenzidine oxidation Armoracia rusticana
1.11.1.7 2-amino-4-nitrophenol competitive inhibition of 3,3',5,5'-tetramethylbenzidine oxidation Armoracia rusticana
1.11.1.7 gallic acid competetive inhibition of 3,3',5,5'-tetramethylbenzidine oxidation Armoracia rusticana
1.11.1.7 resorcinol competitive inhibition of 3,3',5,5'-tetramethylbenzidine oxidation Armoracia rusticana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.7 0.09
-
 3,3',5,5'-tetramethylbenzidine 20°C, pH 7.4 Armoracia rusticana
1.11.1.7 0.09
-
 3,3',5,5'-tetramethylbenzidine 20°C, pH 6.8 Armoracia rusticana
1.11.1.7 0.15
-
 3,3',5,5'-tetramethylbenzidine 20°C, pH 6.4 Armoracia rusticana
1.11.1.7 0.38
-
 o-phenylenediamine 20°, pH 6.2 Armoracia rusticana

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.7 Armoracia rusticana
-
 horseradish
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.7 3,3',5,5'-tetramethylbenzidine + H2O2
-
 Armoracia rusticana ? + H2O
-
 ?
1.11.1.7 5-aminosalicylic acid + H2O2
-
 Armoracia rusticana ? + H2O
-
 ?
1.11.1.7 o-phenylenediamine + H2O2
-
 Armoracia rusticana ? + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.11.1.7 HP acidic isoform, type A Armoracia rusticana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.7 172
-
 3,3',5,5'-tetramethylbenzidine 20°C, pH 7.4 Armoracia rusticana
1.11.1.7 435
-
 3,3',5,5'-tetramethylbenzidine 20°C, pH 6.8 Armoracia rusticana
1.11.1.7 528
-
 o-phenylenediamine 20°, pH 6.2 Armoracia rusticana
1.11.1.7 790
-
 3,3',5,5'-tetramethylbenzidine 20°C, pH 6.4 Armoracia rusticana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.7 6
-
 oxidation of 5-aminosalycylic acid Armoracia rusticana

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.1.7 3 7.5 oxidation of 5-aminosalicylic acid, approx. 30% of maximal activity at pH 4.5 and pH 7.0, respectively Armoracia rusticana