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Literature summary extracted from

  • Takakuwa, N.; Kinoshita, M.; Oda, Y.; Ohnishi, M.
    Isolation and characterization of the genes encoding DELTA8-sphingolipid desaturase from Saccharomyces kluyveri and Kluyveromyces lactis (2002), Curr. Microbiol., 45, 459-461.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.19.4 expression in Saccharomyces cerevisiae Lachancea kluyveri
1.14.19.4 expression in Saccharomyces cerevisiae Kluyveromyces lactis
1.14.19.18 expressed in Saccharomyces cerevisiae Lachancea kluyveri
1.14.19.18 expressed in Saccharomyces cerevisiae Kluyveromyces lactis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.19.18 66500
-
 x * 66500, calculated from amino acid sequence Lachancea kluyveri
1.14.19.18 66500
-
 x * 66500, calculated from amino acid sequence Kluyveromyces lactis
1.14.19.18 67100
-
 x * 67100, calculated from amino acid sequence Lachancea kluyveri
1.14.19.18 67100
-
 x * 67100, calculated from amino acid sequence Kluyveromyces lactis

Organism

EC Number Organism UniProt Comment Textmining
1.14.19.4 Kluyveromyces lactis Q8NKG8
-
-
1.14.19.4 Lachancea kluyveri Q8NKG9 IFO 1685
-
1.14.19.18 Kluyveromyces lactis Q8NKG8
-
-
1.14.19.18 Kluyveromyces lactis IFO 1090 Q8NKG8
-
-
1.14.19.18 Lachancea kluyveri Q8NKG9
-
-
1.14.19.18 Lachancea kluyveri IFO 1685 Q8NKG9
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.19.4 4-hydroxysphinganine + reduced acceptor + H2O
-
 Kluyveromyces lactis 4-hydroxy-trans-8-sphingenine + acceptor + H2O
-
 ?
1.14.19.4 4-hydroxysphinganine + reduced acceptor + H2O
-
 Kluyveromyces lactis IFO 1090 4-hydroxy-trans-8-sphingenine + acceptor + H2O
-
 ?
1.14.19.18 4-hydroxysphinganine + ferrocytochrome b5 + O2 + H+
-
 Kluyveromyces lactis 4-hydroxy-trans-8-sphingenine + ferricytochrome b5 + H2O
-
 ?
1.14.19.18 4-hydroxysphinganine + ferrocytochrome b5 + O2 + H+
-
 Kluyveromyces lactis IFO 1090 4-hydroxy-trans-8-sphingenine + ferricytochrome b5 + H2O
-
 ?
1.14.19.18 additional information no activity with 4-hydroxysphinganine Lachancea kluyveri ?
-
 ?
1.14.19.18 additional information no activity with 4-hydroxysphinganine Lachancea kluyveri IFO 1685 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.19.18 ? x * 66500, calculated from amino acid sequence Lachancea kluyveri
1.14.19.18 ? x * 66500, calculated from amino acid sequence Kluyveromyces lactis
1.14.19.18 ? x * 67100, calculated from amino acid sequence Lachancea kluyveri
1.14.19.18 ? x * 67100, calculated from amino acid sequence Kluyveromyces lactis

Synonyms

EC Number Synonyms Comment Organism
1.14.19.4 DELTA8-sphingolipid desaturase
-
 Lachancea kluyveri
1.14.19.4 DELTA8-sphingolipid desaturase
-
 Kluyveromyces lactis
1.14.19.18 DELTA8-sphingolipid desaturase
-
 Lachancea kluyveri
1.14.19.18 DELTA8-sphingolipid desaturase
-
 Kluyveromyces lactis

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.19.18 cytochrome b5
-
 Lachancea kluyveri
1.14.19.18 cytochrome b5
-
 Kluyveromyces lactis