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Literature summary extracted from

  • Zamolodchikova, T.S.; Sokolova, E.A.; Alexandrov, S.L.; Mikhaleva, II; Prudchenko, I.A.; Morozov, I.A.; Kononenko, N.V.; Mirgorodskaya, O.A.; Da, U.; Larionova, N.I.; Pozdnev, V.F.; Ghosh, D.; Duax, W.L.; Vorotyntseva, T.I.
    Subcellular localization, substrate specificity and crystallization of duodenase, a potential activator of enteropeptidase (1997), Eur. J. Biochem., 249, 612-621.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.21.B3 hanging-drop vapour-diffusion method, protein solution: 10 mg/ml, 50 mM K/Na phosphate, pH 6.7, 1 mM NaN3, 10-14% polyethylene glycol 3350, 4°C, three-dimensional structure determination by X-ray diffraction and analysis Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.B3 0.087
-
 LVTQEVSPKIVGGS pH 8.0, 37°C Bos taurus
3.4.21.B3 0.2
-
 alpha-N-t-butoxycarbonyl-Gly-Phe-Arg-4-nitroanilide pH 8.0, 37°C Bos taurus
3.4.21.B3 0.39
-
 alpha-N-succinyl-Ser-Thr-Pro-Lys-4-nitroanilide pH 8.0, 37°C Bos taurus
3.4.21.B3 0.54
-
 alpha-N-benzoyl-Val-Gly-Arg-4-nitroanilide pH 8.0, 37°C Bos taurus
3.4.21.B3 1
-
 alpha-N-succinyl-Ser-Pro-Lys-4-nitroanilide pH 8.0, 37°C Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.21.B3 Golgi apparatus
-
 Bos taurus 5794
-
3.4.21.B3 rough endoplasmic reticulum
-
 Bos taurus 5791
-
3.4.21.B3 secretory granule
-
 Bos taurus 30141
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.B3 polypeptide + H2O Bos taurus
-
 peptides
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.B3 Bos taurus P80219
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.B3 Brunner's gland excretory duct Bos taurus
-
3.4.21.B3 duodenum
-
 Bos taurus
-
3.4.21.B3 epithelium secretory cells of Brunner's gland Bos taurus
-
3.4.21.B3 mucosa
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.B3 alpha-N-benzoyl-Val-Gly-Arg-4-nitroanilide + H2O
-
 Bos taurus alpha-N-benzoyl-Val-Gly-Arg + 4-nitroaniline
-
 ?
3.4.21.B3 alpha-N-succinyl-Ser-Pro-Lys-4-nitroanilide + H2O
-
 Bos taurus alpha-N-succinyl-Ser-Pro-Lys + 4-nitroaniline
-
 ?
3.4.21.B3 alpha-N-succinyl-Ser-Thr-Pro-Lys-4-nitroanilide + H2O
-
 Bos taurus alpha-N-succinyl-Ser-Thr-Pro-Lys + 4-nitroaniline
-
 ?
3.4.21.B3 alpha-N-succinyl-Thr-Pro-Lys-4-nitroanilide + H2O
-
 Bos taurus alpha-N-succinyl-Thr-Pro-Lys + 4-nitroaniline
-
 ?
3.4.21.B3 alpha-N-t-butoxycarbonyl-Gly-Phe-Arg-4-nitroanilide + H2O
-
 Bos taurus N-t-butoxycarbonyl-Gly-Phe-Arg + 4-nitroaniline
-
 ?
3.4.21.B3 alpha-N-tosyl-Gly-Pro-Lys-4-nitroanilide + H2O
-
 Bos taurus alpha-N-tosyl-Gly-Pro-Lys + 4-nitroaniline
-
 ?
3.4.21.B3 angiotensin II + H2O enzyme cleaves bond between residues 2-3 and 4-5 Bos taurus fragments of angiotensin II
-
 ?
3.4.21.B3 angiotensinogen-(1-14) + H2O enzyme cleaves bond between residues 8-9 Bos taurus fragments of angiotensinogen-(1-14)
-
 ?
3.4.21.B3 bovine duodenase + H2O enzyme cleaves bond between residues 14-15, 17-18, 63-64, 70-71, 110-111, 138-139, 157-158, 162-163, 174-175 and 197-198 Bos taurus fragments of bovine duodenase
-
 ?
3.4.21.B3 bovine serum albumin + H2O enzyme cleaves bond between residues 27-28, 41-42, 64-65, 210-211, 223-224, 231-232, 238-239, and 409-410 Bos taurus fragments of bovine serum albumin
-
 ?
3.4.21.B3 Escherichia coli La-protease + H2O enzyme cleaves bond between residues 219-220 and 239-240 Bos taurus fragments of Escherichia coli La-protease
-
 ?
3.4.21.B3 glucagon + H2O enzyme cleaves bond between residues 12-13 and 18-19 Bos taurus fragments of glucagon
-
 ?
3.4.21.B3 human gamma-interferon + H2O enzyme cleaves bond between residues 69-70 Bos taurus fragments of human gamma-interferon
-
 ?
3.4.21.B3 human interleukin II-(59-79) + H2O enzyme cleaves bond between residues 66-67 Bos taurus fragments of human interleukin II-(59-79)
-
 ?
3.4.21.B3 LVTQEVSPKIVGGS + H2O
-
 Bos taurus LVTQEVSPK + IVGGS
-
 ?
3.4.21.B3 melittin + H2O enzyme cleaves bond between residues 6-7, 7-8, 9-10, and 22-23 Bos taurus fragments of melittin
-
 ?
3.4.21.B3 Naja naja oxiana neurotoxin II + H2O enzyme cleaves bond between residues 15-16, 26-27, 44-45, and 32-33 Bos taurus fragments of Naja naja oxiana neurotoxin II
-
 ?
3.4.21.B3 polypeptide + H2O
-
 Bos taurus peptides
-
 ?
3.4.21.B3 porcine proinsulin + H2O enzyme cleaves bond between residues 29-30, 31-32, 32-33, 56-57, 64-65 and 66-67 Bos taurus fragments of porcine proinsulin
-
 ?
3.4.21.B3 surfagon + H2O enzyme cleaves bond between residues 3-4 Bos taurus fragments of surfagon
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.B3 monomer
-
 Bos taurus

Synonyms

EC Number Synonyms Comment Organism
3.4.21.B3 duodenase I
-
 Bos taurus
3.4.21.B3 duodenase serine protease
-
 Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.B3 37
-
-
 Bos taurus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.B3 6
-
 alpha-N-benzoyl-Val-Gly-Arg-4-nitroanilide pH 8.0, 37°C Bos taurus
3.4.21.B3 24
-
 alpha-N-t-butoxycarbonyl-Gly-Phe-Arg-4-nitroanilide pH 8.0, 37°C Bos taurus
3.4.21.B3 84
-
 LVTQEVSPKIVGGS pH 8.0, 37°C Bos taurus
3.4.21.B3 156
-
 alpha-N-succinyl-Ser-Pro-Lys-4-nitroanilide pH 8.0, 37°C Bos taurus
3.4.21.B3 438
-
 alpha-N-succinyl-Ser-Thr-Pro-Lys-4-nitroanilide pH 8.0, 37°C Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.B3 8
-
-
 Bos taurus