EC Number | Application | Comment | Organism |
---|---|---|---|
1.17.1.4 | synthesis | expression of genes xdhAB encoding the two subunits of enzyme, in Escherichia coli, produces active enzyme with moleybdenum content of 0.11-0.16 mol per alphabeta protomer and iron and FAD levels at stoichiometries similar to native enzyme. Coexpression of xdhAB genes with Pseudomonas aeruginosa xdhC gene increases level of molybdenum incorporated to a 1:1 stoichiometry and results in high levels of functional protein, up to 2284 units per mg and 8039 mg per l | Delftia acidovorans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.17.1.4 | 0.066 | - |
xanthine | coexpression of genes xdhAB, conditions of high aeration, pH 7.8, 25°C | Delftia acidovorans | |
1.17.1.4 | 0.067 | - |
xanthine | coexpression of genes xdhAB, conditions of low aeration, pH 7.8, 25°C | Delftia acidovorans | |
1.17.1.4 | 0.085 | - |
xanthine | coexpression of genes xdhABC, conditions of high aeration, pH 7.8, 25°C | Delftia acidovorans | |
1.17.1.4 | 0.1 | - |
NAD+ | coexpression of genes xdhABC, conditions of high aeration, pH 7.8, 25°C | Delftia acidovorans | |
1.17.1.4 | 0.113 | - |
NAD+ | coexpression of genes xdhABC, conditions of low aeration, pH 7.8, 25°C | Delftia acidovorans | |
1.17.1.4 | 0.148 | - |
NAD+ | coexpression of genes xdhAB, conditions of high aeration, pH 7.8, 25°C | Delftia acidovorans | |
1.17.1.4 | 0.156 | - |
NAD+ | coexpression of genes xdhAB, conditions of low aeration, pH 7.8, 25°C | Delftia acidovorans | |
1.17.1.4 | 80.09 | - |
xanthine | coexpression of genes xdhABC, conditions of low aeration, pH 7.8, 25°C | Delftia acidovorans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.17.1.4 | Delftia acidovorans | Q8RLC0 | large subunit; expression of genes xdhAB encoding the two subunits of enzyme, in Escherichia coli, produces active enzyme. Coexpression of xdhAB genes with Pseudomonas aeruginosa xdhC gene results in high levels of functional protein | - |
1.17.1.4 | Delftia acidovorans | Q8RLC1 | small subunit; expression of genes xdhAB encoding the two subunits of enzyme, in Escherichia coli, produces active enzyme. Coexpression of xdhAB genes with Pseudomonas aeruginosa xdhC gene results in high levels of functional protein | - |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.17.1.4 | xanthine + NAD+ + H2O | - |
Delftia acidovorans | urate + NADH | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.17.1.4 | 0.4 | - |
xanthine | coexpression of genes xdhAB, conditions of low aeration, pH 7.8, 25°C | Delftia acidovorans | |
1.17.1.4 | 27 | - |
xanthine | coexpression of genes xdhAB, conditions of high aeration, pH 7.8, 25°C | Delftia acidovorans | |
1.17.1.4 | 86 | - |
xanthine | coexpression of genes xdhABC, conditions of low aeration, pH 7.8, 25°C | Delftia acidovorans | |
1.17.1.4 | 118 | - |
xanthine | coexpression of genes xdhABC, conditions of high aeration, pH 7.8, 25°C | Delftia acidovorans |