Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Jensen, K.F.; Arent, S.; Larsen, S.; Schack, L.
    Allosteric properties of the GTP activated and CTP inhibited uracil phosphoribosyltransferase from the thermoacidophilic archaeon Sulfolobus solfataricus (2005), FEBS J., 272, 1440-1453.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.2.9 GTP strong stimulation, binds cooperatively with 5-phosphoribosyl 1-diphosphate Saccharolobus solfataricus
2.4.2.9 additional information the enzyme undergoes a transition from a weakly active or inactive T-state, favored by binding of UMP and CTP, to an active R-state, favored by binding of GTP and 5-phosphoribosyl 1-diphosphate Saccharolobus solfataricus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.9 expression in Escherichia coli Saccharolobus solfataricus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.9 CTP powerful inhibitor in combination with UMP. CTP and UMP can be bound simultaneously Saccharolobus solfataricus
2.4.2.9 additional information the enzyme undergoes a transition from a weakly active or inactive T-state, favored by binding of UMP and CTP, to an active R-state, favored by binding of GTP and 5-phosphoribosyl 1-diphosphate Saccharolobus solfataricus
2.4.2.9 UMP powerful inhibitor in combination with CTP. CTP and UMP can be bound simultaneously Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.9 additional information
-
 additional information
-
 Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.9 98000
-
 sucrose density gradient centrifugation Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.9 Saccharolobus solfataricus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.9 uracil + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Saccharolobus solfataricus UMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.9 tetramer
-
 Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
2.4.2.9 UPRTase
-
 Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.9 0.36
-
 5-phospho-alpha-D-ribose 1-diphosphate unactivated enzyme Saccharolobus solfataricus
2.4.2.9 0.36
-
 Uracil unactivated enzyme Saccharolobus solfataricus
2.4.2.9 6.1
-
 5-phospho-alpha-D-ribose 1-diphosphate activated by 1 mM GTP Saccharolobus solfataricus
2.4.2.9 6.1
-
 Uracil activated by 1 mM GTP Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.9 5.5
-
 at 60°C Saccharolobus solfataricus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.2.9 5.5 8 pH 3.8: no activity, pH 5.5: optimum, pH 8: about 20% of maximal activity Saccharolobus solfataricus